Teaching Old Clicks New Tricks
Triple, Mutually Orthogonal Bioorthogonal Pairs through the Design of Electronically Activated Sulfamate-Containing Cycloalkynes.
J. Am. Chem. Soc. 2020;
The introduction of bioorthogonal reactions has enabled tremendous advances in chemical biology. The authors designed two new, mutually orthogonal pairs by electronic tuning of S-, N-, and O-containing cyclooctynes (SNO-OCTs). The reported system should prove to be a useful tool for future in cellulo investigations.
Using computational and experimental studies, the authors were able to design cyclooctynes with orthogonal reactivities. By including a previously reported boronic acid/hydrazine pair, the authors were able to obtain a triple ligation system and demonstrated its utility through protein and cellular labeling assays.
16. Dezember 2020 (online)
© 2020. Thieme. All rights reserved
Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany