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J Pediatr Intensive Care 2020; 09(04): 265-270
DOI: 10.1055/s-0040-1710587
Original Article

The Ameliorative Effects of Ascorbic Acid on Critical Illness Polyneuropathy in Rodent Sepsis Model

Eda Sunnetci Silistre
1   Department of Pediatric, Istanbul Training and Research Hospital, Istanbul, Turkey
,
Oytun Erbas
2   Department of Physiology, Bilim University Medical Faculty, Istanbul, Turkey
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Funding None.
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Abstract

Although the exact pathophysiology of critical illness polyneuropathy (CIP) is still unknown, there are several hypotheses, some of which are increased inflammation and oxidative stress. We used rodent sepsis model in which we induced sepsis through cecal ligation followed by cecal puncture. We then administered ascorbic acid (AA) and evaluated outcomes. The levels of malondialdehyde (MDA), tumor necrosis factor α (TNF-α), interleukins (IL)-6 in the plasma, and heat shock protein-70 (HSP-70) levels in the sciatic nerve were measured, and also electromyography analyses were performed. While plasma MDA, TNF-α, and IL-6 levels were decreased significantly with AA treatment, sciatic nerve levels of HSP-70 were significantly elevated in the AA group. A significant increase in compound muscle action potential (CMAP) amplitude and a significant decrease in CMAP latency were detected in the AA group. We observed healing effects of AA on a rat model of CIP and these effects seem to be related to its anti-inflammatory and antioxidant properties.

Keywords

critical illness polyneuropathy - sepsis - ascorbic acid - malondialdehyde - compound muscle action potential


Publication History

Received: 11 March 2020

Accepted: 15 April 2020

Article published online:
22 May 2020

© 2020. Thieme. All rights reserved.

Georg Thieme Verlag KG
Stuttgart · New York

 

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