Planta Med 2019; 85(18): 1545
DOI: 10.1055/s-0039-3400078
Main Congress Poster
Poster Session 2
© Georg Thieme Verlag KG Stuttgart · New York

Recovery effect of a monogalactosy imonoacylglycerol from the brown alga Agarum clathratum subsp. yakishiriense on neomycin-induced hair cell damage in zebrafish

Y Jeon
1   School of Pharmacy, Sungkyunkwan University,, 16419, Suwon,Republic of Korea
,
YH Nam
2   Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University,, 17104, Gyeonggi-do Republic of Korea
,
TH Kang
2   Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University,, 17104, Gyeonggi-do Republic of Korea
,
JH Kwak
1   School of Pharmacy, Sungkyunkwan University,, 16419, Suwon,Republic of Korea
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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Marine plants are popular and abundant food ingredients mainly in Asian countries, and also known to have many beneficial biological activities. Therefore, the development of useful materials from marine plants is one of important contributions to human health. For this purpose, we previously investigated numerous marine plants on various biological activities. As a result, Agarum clathratum subsp. yakishiriense showed amelorative effect in the zebrafish model for hearing loss. A. clathratum subsp. yakishiriense Yamada ex G.H. Boo & P. C. Silva is a perennial brown alga in the family Costariaceae and is widely distributed in the coastal area of the East Sea in Korea [1]. The EtOH extract of A. clathratum subsp. yakishiriense was suspended in water and then partitioned consecutively with CH2Cl2, EtOAc, and n-BuOH. Among these fractions, the EtOAc fraction was subjected to column chromatographic separation. Two new and a known monogalactosylmonoacylglycerols were isolated from the EtOAc fraction. The structures of two new monogalactosylacyl glycerols were determined as (2S)-1-O-(6Z,9Z,12Z,15Z-hexadeca- tetraenoyl)-3-O-β-D-galactopyranosyl glycerol (1) and (2S)-1-O-(6Z,9Z,12Z-hexadecatrienoyl)-3-O-β-D-galactopyranosyl glycerol (2) from spectral data and chemical evidence. A known compound, (2S)-1-O-(6Z,9Z,12Z-hexadecatetraenoyl)-3-O-β-D-galactopyranosylglycerol (3), was identified by comparing its spectral data with literature values. The isolated compounds were investigated the amelorative effect on neomycin-induced hair cell damage in zebrafish. Compound 3 showed significant recovery on otic hair cell damage at concentration of 0.1 μM. In conclusion, (2S)-1-O-(6Z,9Z,12Z-hexadecatetraenoyl)-3-O-β-D-galactopyranosylglycerol (3) revealed potent recovery effect for the hearing loss.