Melissa officinalis essential oil loaded glycerosomes: preparation and in vitro activity evaluation against herpes labialis (HSV-1)

G Vanti; V Dourdouni; D Lazari; C Panagiotidis; SG Ntallis; S Litsiou; C Patsoura; V Piazzini; L Risaliti; MC Bergonzi; AR Bilia

doi:10.1055/s-0039-3399654

Planta Medica, Table of Contents

Planta Med 2019; 85(18): 1396-1397
DOI: 10.1055/s-0039-3399654

Pre-Congress Symposia

Young Researchers’ Workshop

Melissa officinalis essential oil loaded glycerosomes: preparation and in vitro activity evaluation against herpes labialis (HSV-1)

Authors

G Vanti

¹Department of Chemistry, University of Florence,, Via Ugo Schiff 6, 50019 Sesto Fiorentino (Florence), Italy
V Dourdouni

²Laboratory of Pharmacology, Department of Pharmacognosy/Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki,, Thessaloniki 54124, Greece
D Lazari

²Laboratory of Pharmacology, Department of Pharmacognosy/Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki,, Thessaloniki 54124, Greece
C Panagiotidis

²Laboratory of Pharmacology, Department of Pharmacognosy/Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki,, Thessaloniki 54124, Greece
SG Ntallis

²Laboratory of Pharmacology, Department of Pharmacognosy/Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki,, Thessaloniki 54124, Greece
S Litsiou

²Laboratory of Pharmacology, Department of Pharmacognosy/Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki,, Thessaloniki 54124, Greece
C Patsoura

²Laboratory of Pharmacology, Department of Pharmacognosy/Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki,, Thessaloniki 54124, Greece
V Piazzini

¹Department of Chemistry, University of Florence,, Via Ugo Schiff 6, 50019 Sesto Fiorentino (Florence), Italy
L Risaliti

¹Department of Chemistry, University of Florence,, Via Ugo Schiff 6, 50019 Sesto Fiorentino (Florence), Italy
MC Bergonzi

¹Department of Chemistry, University of Florence,, Via Ugo Schiff 6, 50019 Sesto Fiorentino (Florence), Italy
AR Bilia

¹Department of Chemistry, University of Florence,, Via Ugo Schiff 6, 50019 Sesto Fiorentino (Florence), Italy

Abstract

Full Text

Essential oils (EOs) are complex mixtures of strongly active compounds, but very volatile and sensitive to light, oxygen, moisture and temperature. Loading inside nanostructures can be a strategy to stabilize them and use them in therapy [1]. In the present study, Melissa officinalis L. (Lamiaceae) essential oil was loaded inside glycerosomes (GS) at a concentration of 10 µL/mL and was evaluated for its anti-herpetic activity [2]. GS were prepared by the thin layer evaporation method and characterized by Light Scattering techniques, determining average diameter (about 60 nm), polydispersity index (⋍ 0.2) and ζ-potential (⋍ -30 mV). From the morphological observation by transmission electron microscope, GS appeared as small vesicles with several lamellae and a spherical shape. Moreover, the EO encapsulation efficiency inside the GS, in terms of citral and β-caryophyllene, obtained by HPLC-DAD, was ⋍63 % and ⋍76 % respectively and the EO release, by the dialysis bag method, was very low (⋍15% of citral) within 24h. The same instruments and analytical techniques were adopted to monitor the GS long-term stability until 4 months and no relevant changes were observed in the chemical-physical parameters. Successively, GS were tested with an in vitro antiviral assay against the HSV-1/strain vCLIDA61. From these studies, the antiviral activity of loaded-EO was found to be comparable to free-EO, for high concentrations of EO. Hence, at present, GS seems to be a promising tool in order to administer the EO and replace the conventional anti-herpetic drugs, when drug-resistance forms take place.

References

References
1 Bilia AR, Guccione C, Isacchi B, Righeschi C, Firenzuoli F, Bergonzi MC. Evidence-Based Complementary and Alternative Medicine. 2014
2 Schnitzler P, Schuhmacher A, Astani A, Reichling J. Phytomedicine 2008; 15 (09) : 734-740.