Influence of Plasma “Age” and Anticoagulant on Factor VIII Content of Plasma and on the Yield of an Intermediate-Purity Concentrate.

Claims made for the higher factor VIII content of plasma taken into particular anticoagulants or separated mithin a certain time of donation are not necessarily relevant to concentrates carried through several stages of processing. Four types of plasma were collected; from blood taken Into ACD or CPD anticoagulant and separated within 6-8h (FF) or after overnight (ON) storage of blood donations. Approximately 24 donations were pooled into 5 1 polyethylene packs and froien for storage below -30°. Before fractionation, packs were sampled by “coring” and the factor VIII content of the cores was measured. CPD/FF plasma had a significantly higher content of factor VIII than either ACD/FF or ACD/ON plasma, but for either anticoagulant the difference between FF and ON plasma was not significant.

Intermediate purity concentrate was prepared in ≃ 100 kg batches by large-scale cryopreeipitation, adsorption and precipitation of unwanted proteins at reduced pH and temperature. Batches of concentrate made from CPD plasma gave a significantly higher yield of factor VIII and higher specific activity than those made from ACD plasma, but there were fewer and smaller differences between batches from FF and ON plasma in either anticoagulant.

 

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