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Thromb Haemost 1977; 38(01): 339
DOI: 10.1055/s-0039-1682480
Symposia Session
III Molecular problems of factor IX
Schattauer GmbH

Electroimmunoassay of Factor IX in the Detection of Haemophilia B Carriers

Authors

  • F. Panicucci

    1   Haemophilia Centre, St. Chiara Hospital, University of Pisa, Pisa, Italy

  • U. Baicchi

    1   Haemophilia Centre, St. Chiara Hospital, University of Pisa, Pisa, Italy

  • A. Sagripanti

    1   Haemophilia Centre, St. Chiara Hospital, University of Pisa, Pisa, Italy

  • E. Pinori

    1   Haemophilia Centre, St. Chiara Hospital, University of Pisa, Pisa, Italy
Further Information

Publication History

Publication Date:
16 April 2019 (online)

 
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    Parallel determinations of factor IX activity and factor IX antigen were carried out on 28 haemophilia B carriers and on 20 normal women. Factor IX activity was measured by a one stage method. Factor IX antigen was quantified by electroimmuno assay in agarose gel containing heterologous monospecific antiserum against human factor IX.

    The activity was observed to be at the same level as the antigen in normal women. Discrepancy was not found between the antigen and the activity in almost all of carriers: only in the mothers of haemophiliacs Bor B+ or BM the factor IX antigen resulted greater than that activity. Our results show that electroimmuno-assay may be used to study carriers of haemophilia B genetic variants and confirm that in the majority of cases they can probably be diagnosed on the basis of factor IX activity alone.


     

     


     
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