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Thromb Haemost 1977; 38(01): 38
DOI: 10.1055/s-0039-1680427
Mixed Posters II
Coagulation
Schattauer GmbH

Some Characteristics of Fibrin Monomer Preparations Made from Dissolved Fibrin Clots or from Fibrinogen and Immobilized Thrombin in Urea

Authors

  • F. Brosstad

    1   Haematological Research Laboratory, Department IX, Ullevål Hospital University Clinic, Oslo, Norway

  • H. C. Godal

    1   Haematological Research Laboratory, Department IX, Ullevål Hospital University Clinic, Oslo, Norway

  • P. Kierulf

    1   Haematological Research Laboratory, Department IX, Ullevål Hospital University Clinic, Oslo, Norway
Further Information

Publication History

Publication Date:
16 April 2019 (online)

 
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    Fibrin monomer was prepared from purified human fibrinogen by the action of insolubilized thrombin in the presence of 3.3 M urea. As judged by N-terminal amino acid analyses, complete conversion of fibrinogen to fibrin was achieved. The preparation proved homogeneous by gel chromatography and SDS electrophoresis and had retained clotting abilities identical to those of fibrin monomers arising from fibrinogen exposed to large amounts of thrombin. No changes were observed after freezing in liquid nitrogen or after storage at +4°C for 4 weeks. As to homogeneity and ability to polymerize the preparation proved superior to those fibrin monomer preparations obtained by dissolution of polymerized fibrin in various solvents.


     

     


     
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