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Thromb Haemost 1957; 01(01): 001-008
DOI: 10.1055/s-0038-1656157
DOI: 10.1055/s-0038-1656157
Originalarbeiten – Original Article – Travaux Originaux
A Study of the Separation of Fibrinogen and Antihemophilic Factor (AHF) in Canine, Porcine, and Human Plasmas
This investigation was supported in part by a grant, H-1648, from the National Heart Institute of the National Institutes of Health, Public Health Service.
Further Information
Publication History
Publication Date:
05 June 2018 (online)
Summary
1. Studies are reported on attempts to separate antihemophilic factor (AHF) from fibrinogen in plasma by selective adsorption with fuller’s earth, aluminum hydroxide gel, and Seitz filtration.
2. Fuller’s earth adsorption of canine plasma resulted in almost complete removal of fibrinogen without loss of AHF. By the use of this procedure as part of the fractionation procedure, potent AHF fractions free of fibrinogen have been obtained.
3. Al(OH)3 adsorption of fibrinogen from bovine, canine, and human plasmas is greater than the adsorption of AHF. Al(OH)3 adsorption offers promise for the preparation of fibrinogen-poor AHF fractions of human or bovine plasma.
4. Seitz filtration of plasma causes loss of AHF with little or no loss of fibrinogen.
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References
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- 2 Sahíi H. Über das Wesen der Hämophilie. Z. klin. Med. 56: 264 1905;
- 3 Addis T. The Pathogenesis of Hereditary Haemophilia. J. Pathol. Bact. 15: 427 1911;
- 4 Brinkhous K. M. A Study of the Clotting Defect in Hemophilia: The Delayed Formation of Thrombin. Amer. J. Med. Sci. 198: 509 1939;
- 5 Lewis J. H, Soulier J. P, and Taylor F. H. L. Chemical, Clinical and Immunological Studies on the Products of Human Plasma Fractionation. XXXIII. The Coagulation Defect in Hemophilia: The Effect in Vitro and in Vivo on the Coagulation Time in Hemophilia of a Prothrombin and Fibrinogen-free Normal Plasma and its Derived Protein Fractions. J. clin. Invest. 25: 876 1946;
- 6 Alexander B, and Landwehr G. Studies of Hemophilia. II. The Assay of the Antihemophilic Clot-Promoting Principle in Normal Human Plasma with Some Observations on the Relative Potency of Certain Plasma Fractions. J. clin. Invest. 27: 98 1948;
- 7 Spaet T. H, and Kinsell B. G. Properties of Bovine Anti-Hemophilic Factor Proc. Soc. exp. Biol. 84: 314 1953;
- 8 Bidwell E. The Purification of Bovine Antihaemophilic Globulin. Brit. J. Haematol. 1: 35 1955;
- 9 Van Creveld S, Hoorweg P. G, Den Ottolander G. J. H, and Veder H. A. Isolation of the Anti-Haemophilic Factor from Human Plasma. Acta haemat. (Basel) 15: 1 1956;
- 10 Biggs R, and Macfarlane R. G. Human Blood Coagulation. Blackwell Sci. Pub.; Oxford: 340 1953
- 11 Langdell R. D, Wagner R. H, and Brinkhous K. M. Effect of Antihemophilic Factor on One-Stage Clotting Tests. J. Lab. clin. Med. 41: 637 1953;
- 12 Graham J. B, Collins Jr. D. L, Godwin I. D, and Brinkhous K. M. Assay of Plasma Antihemophilic Activity in Normal, Heterozygous (Hemophilia) and Prothrombinopenic Dogs. Proc. Soc. exp. Biol. 77: 294 1951;
- 13 Shinowara G. Y, and Rosenfeld L. Enzyme Studies on Human Blood. VIII. The Effect of Fibrinogen Concentration on the Thrombin Clotting Time. J. Lab. clin. Med. 37: 303 1951;
- 14 Shinowara G. Y. Enzyme Studies on Human Blood. III. Effect of Plasma Proteins on Coagulation. J. Lab. clin. Med. 34: 477 1949;
- 15 Graham J. B, Buckwalter J. A, Hartley L. J, and Brinkhous K. M. Canine Hemophilia, Observations on the Course, the Clotting Anomaly, and the Effect of Blood Transfusions. J. exp. Med. 90: 97 1949;
- 16 Langdell R. D, Wagner R. H, and Brinkhous K. M. Antihemophilic Factor (AHF) Levels Following Transfusions of Blood, Plasma Fractions. Proc. Soc. exp. Biol. 88: 212 1955;
- 17 Graham J. B, Langdell R. D, Morrison Jr. F. C, and Brinkhous K. M. Serum Accelerator Factors and Antihemophilic Factor (AHF) in Early Phases of Clotting. Proc. Soc. exp. Biol. 87: 45 1954;
- 18 Wagner R. H, Pate D, and Brinkhous K. M. Further Purification of Antihemophilic Factor (AHF) from Dog Plasma. Fed. Proc. 13: 445 1954;
- 19 Brinkhous K. M. Plasma Antihemophilic Factor: Biological and Clinical Aspects. Sang 25: 738 1954;
- 20 Wagner R. H, Brannan Jr. W. M, and Brinkhous K. M. Antiaccelerator (Anticonvertin) Activity of Canine Plasma and Serum. Proc. Soc. exp. Biol. 89: 266 1955;
-
References
- 1 Alexander B, Goldstein R, Rich L, Le Bolloc’h A. G, Diamond L. K, and Borges W. Congenital Afibrinogenemia, A Study of Some Basic Aspects of Coagulation. Blood 9: 843 1954;
- 2 Sahíi H. Über das Wesen der Hämophilie. Z. klin. Med. 56: 264 1905;
- 3 Addis T. The Pathogenesis of Hereditary Haemophilia. J. Pathol. Bact. 15: 427 1911;
- 4 Brinkhous K. M. A Study of the Clotting Defect in Hemophilia: The Delayed Formation of Thrombin. Amer. J. Med. Sci. 198: 509 1939;
- 5 Lewis J. H, Soulier J. P, and Taylor F. H. L. Chemical, Clinical and Immunological Studies on the Products of Human Plasma Fractionation. XXXIII. The Coagulation Defect in Hemophilia: The Effect in Vitro and in Vivo on the Coagulation Time in Hemophilia of a Prothrombin and Fibrinogen-free Normal Plasma and its Derived Protein Fractions. J. clin. Invest. 25: 876 1946;
- 6 Alexander B, and Landwehr G. Studies of Hemophilia. II. The Assay of the Antihemophilic Clot-Promoting Principle in Normal Human Plasma with Some Observations on the Relative Potency of Certain Plasma Fractions. J. clin. Invest. 27: 98 1948;
- 7 Spaet T. H, and Kinsell B. G. Properties of Bovine Anti-Hemophilic Factor Proc. Soc. exp. Biol. 84: 314 1953;
- 8 Bidwell E. The Purification of Bovine Antihaemophilic Globulin. Brit. J. Haematol. 1: 35 1955;
- 9 Van Creveld S, Hoorweg P. G, Den Ottolander G. J. H, and Veder H. A. Isolation of the Anti-Haemophilic Factor from Human Plasma. Acta haemat. (Basel) 15: 1 1956;
- 10 Biggs R, and Macfarlane R. G. Human Blood Coagulation. Blackwell Sci. Pub.; Oxford: 340 1953
- 11 Langdell R. D, Wagner R. H, and Brinkhous K. M. Effect of Antihemophilic Factor on One-Stage Clotting Tests. J. Lab. clin. Med. 41: 637 1953;
- 12 Graham J. B, Collins Jr. D. L, Godwin I. D, and Brinkhous K. M. Assay of Plasma Antihemophilic Activity in Normal, Heterozygous (Hemophilia) and Prothrombinopenic Dogs. Proc. Soc. exp. Biol. 77: 294 1951;
- 13 Shinowara G. Y, and Rosenfeld L. Enzyme Studies on Human Blood. VIII. The Effect of Fibrinogen Concentration on the Thrombin Clotting Time. J. Lab. clin. Med. 37: 303 1951;
- 14 Shinowara G. Y. Enzyme Studies on Human Blood. III. Effect of Plasma Proteins on Coagulation. J. Lab. clin. Med. 34: 477 1949;
- 15 Graham J. B, Buckwalter J. A, Hartley L. J, and Brinkhous K. M. Canine Hemophilia, Observations on the Course, the Clotting Anomaly, and the Effect of Blood Transfusions. J. exp. Med. 90: 97 1949;
- 16 Langdell R. D, Wagner R. H, and Brinkhous K. M. Antihemophilic Factor (AHF) Levels Following Transfusions of Blood, Plasma Fractions. Proc. Soc. exp. Biol. 88: 212 1955;
- 17 Graham J. B, Langdell R. D, Morrison Jr. F. C, and Brinkhous K. M. Serum Accelerator Factors and Antihemophilic Factor (AHF) in Early Phases of Clotting. Proc. Soc. exp. Biol. 87: 45 1954;
- 18 Wagner R. H, Pate D, and Brinkhous K. M. Further Purification of Antihemophilic Factor (AHF) from Dog Plasma. Fed. Proc. 13: 445 1954;
- 19 Brinkhous K. M. Plasma Antihemophilic Factor: Biological and Clinical Aspects. Sang 25: 738 1954;
- 20 Wagner R. H, Brannan Jr. W. M, and Brinkhous K. M. Antiaccelerator (Anticonvertin) Activity of Canine Plasma and Serum. Proc. Soc. exp. Biol. 89: 266 1955;