Thromb Haemost 1963; 10(02): 379-389
DOI: 10.1055/s-0038-1654791
Originalarbeiten — Original Article — Travaux Originaux
Schattauer GmbH

Fibrinolytic Properties of Proteases Derived from Human, Dog and Rabbit Leukocytes[*]

Henry Gans
1   Department of Surgery, University of Minnesota Hospitals, Minneapolis 14, Minnesota
› Author Affiliations
Further Information

Publication History

Publication Date:
22 June 2018 (online)

Summary

Intact and disintegrated human, dog and rabbit leukocytes were studied for their ability to lyse fibrin. It was noted that broken-up rabbit leukocytes fail to lyse bovine or rabbit fibrin. Human and dog leukocytes, on the other hand, readily lyse bovine fibrin.

Prior incubation at pH 1 and pH 8 resulted in maximally active human cell preparations. Similar results were obtained with dog cells upon prior incubation at pH 4 and pH 8. Loss of activity was noted upon prior incubation for 15 minutes at 56° C. Adsorption of human leukocytes with celite and charcoal resulted in a 2 and 21/2 fold increase in activity.

The noted lysis was found to result from proteolytic activity as well as from plasminogen activator activity. The results to these studies suggest that autolysis of relatively few leukocytes will produce a rapid breakdown of considerable amounts of fibrin.

* Supported by U.S.P.H.S. Grant No. H-5341 and a Grant in Aid of the Graduate School of the University of Minnesota.


 
  • 1 Astrup T, Mullertz P. The Fibrin Plate Method for Estimating Fibrinolytic Activity. Arch. Biochem. 11: 309 1952;
  • 2 Dible J. H. Organization and Recanalization of Arterial Thrombosis. J. Path. Bact. 75: 1 1958;
  • 3 Fritze E. Das Abwehrsystem der neutrophilen Granulozyten. Dtsch. med. Wschr. 81: 601 1956;
  • 4 Gans H. Changing Concepts in Thrombosis and Thrombolysis. Surgery. 53: 631 1963;
  • 5 Henry R. L. Personal communication.
  • 6 Jacobsson K. Studies on the Determination of Fibrinogen in Human Plasma. Scand. J. clin. Lab. Invest. 07: 11 (Suppl. 4) 1955;
  • 7 Lassen M. Heat Denaturation of Plasminogen in the Fibrin Plate Method. Acta physiol, scand. 27: 37 1952;
  • 8 Metchnikoff E. Leçons sur pathologie comparée de l’inflammation. Paris. 1892
  • 9 Mounter L. A, Atryeth W. Proteases in Human Leukocytes. Blood. 15: 52 1960;
  • 10 Opie E. L. Enzymes and Anti-Enzymes of Inflammatory Exudate. J. exp. Med. 7: 316 1905; The Enzymes in Phagocytic Cells of Inflammatory Exudates. J. exp. Med. 08
  • 11 Pantlitschko M, Stattmann K. Die proteolytische Enzyme der weißen Blutkörperchen. Biochem. Z. 326: 252 1955;
  • 12 Tabachnick J, Weiss C. h. Mechanism of Softening of Tubercles. Arch. Path. 61: 77 1956;
  • 13 Valentine M. N, Beck W. S. Biochemical Studies on Leukocytes. J. Lab. clin. Med. 38: 39 1957;