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Thromb Haemost 1959; 3(04): 572-577
DOI: 10.1055/s-0038-1654412
DOI: 10.1055/s-0038-1654412
Originalarbeiten — Original Articles — Travaux Originaux
The Separation of AHF from Fibrinogen[*)]
Weitere Informationen
Publikationsverlauf
Publikationsdatum:
12. Juni 2018 (online)
Summary
Anti-haemophilic globulin and fibrinogen of both bovine and human origin could be separated on columns of DEAE-cellulose. The yield of bovine AHF was high in contrast to that of human AHF, but further work to improve this yield is in progress.
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*) This work was aided by grants from the Organization for Pure Scientific Research in the Netherlands.
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References
- 1 Creveld S. van. Sporadische haemophilie. Maandschr. v. Kindergen 1934; 3: 351
- 2 Bendien W. M, Creveld S. van. Investigations on hemophilia. Amer. J. Diseases Childr 1937; 54: 713
- 3 Creveld S. van, Hoorweg P. G, Ottolander G. J. H. den, Veder H. A. Isolation of the anti-haemophilic factor from human plasma. Acta haematol 1956; 15: 1
- 4 Cohn E. J, StrOng Jr L. E, Hughes W. L, Mulford D. J, Aswordth J. N, Melin M, Taylor H. L. Preparation and properties of serum and plasma proteins. IV. A system for the separation into fractions of protein and lipoprotein components of biological tissues and fluids. J. Amer. Chem. Soc 1946; 68: 459
- 5 Seegers W. H, Landaburu R. H, Fenichel R. L. Isolation of platelet co-factor I from plasma and some properties of the preparation. Amer. J. Physiol 1957; 190: 1
- 6 Peters H. J. Papierelectrophorese van liquor cerebrospinalis. Chem. Weekbl 1953; 49: 248
- 7 Clauss A. Méthode rapide de détermination du fibrinogène basée sur le temps de coagulation. Acta haematol 1957; 17: 237
- 8 Peterson E. A, Sober H. A. Chromatography of proteins. I. Cellulose ion-exchange adsorbents. J. Amer. Chem. Soc 1956; 78: 751
- 9 Sober H. A, Gutter F. J, Wijckoff M. M, Peterson E. A. Chromatography of proteins. II. Fractionations of serum protein on anion-exchange cellulose. J. Amer. Chem. Soc 1956; 78: 756
- 10 Fahey J. L, McCoy P. F, Goulian M. Chromatography of serum proteins in normal and pathologic sera: the distribution of protein-bound carbohydrate and cholesterol, siderophilin, thyroxin-binding protein, B12 binding protein, alkaline and acid phosphatases, radioiodinated albumin and myeloma proteins. J. Clin. Invest 1958; 37: 272
- 11 LOzner E. L, Kark R, Taylor F. H. L. The coagulation defect in haemophilia: the clot promoting activity in hemophilia of berkefelded normal human plasma free from fibrinogen and prothrombin. J. Clin. Invest 1939; 18: 603
- 12 Shinowara G. Enzyme studies on human blood XI. The isolation and characterization of thromboplastic cell and plasma components. J. Lab. Clin. Med 1951; 38: 11
- 13 Lorand L, Laki K. A simple method for purifying an activator of prothrombin. Biochim. biophys. Acta 1954; 13: 448
- 14 Wagner R. H, Richardson B. A, Brinkhous K. M. A study of the separation of fibrinogen and antihemophilic factor (AHF) in canine, porcine and human plasmas. Thromb. diath. haem 1957; 1: 1
- 15 Surgenor D. M, Steele B. B. 6th Annual Symposium on blood. January 1957. Thromb. diath. haem 1957; 1: 563
- 16 Soulier J. P, Gobbi F, Larrieu M. J. Séparation du fibrinogène et du facteur antihémophilique A. Rev. d’hématol 1957; 12: 481
- 17 Blombäck M. Purification of antihemophilic globulin I. Some studies on the stability of the antihemophilic globulin activity in fraction I-o and a method for its partial separation from fibrinogen.
-
References
- 1 Creveld S. van. Sporadische haemophilie. Maandschr. v. Kindergen 1934; 3: 351
- 2 Bendien W. M, Creveld S. van. Investigations on hemophilia. Amer. J. Diseases Childr 1937; 54: 713
- 3 Creveld S. van, Hoorweg P. G, Ottolander G. J. H. den, Veder H. A. Isolation of the anti-haemophilic factor from human plasma. Acta haematol 1956; 15: 1
- 4 Cohn E. J, StrOng Jr L. E, Hughes W. L, Mulford D. J, Aswordth J. N, Melin M, Taylor H. L. Preparation and properties of serum and plasma proteins. IV. A system for the separation into fractions of protein and lipoprotein components of biological tissues and fluids. J. Amer. Chem. Soc 1946; 68: 459
- 5 Seegers W. H, Landaburu R. H, Fenichel R. L. Isolation of platelet co-factor I from plasma and some properties of the preparation. Amer. J. Physiol 1957; 190: 1
- 6 Peters H. J. Papierelectrophorese van liquor cerebrospinalis. Chem. Weekbl 1953; 49: 248
- 7 Clauss A. Méthode rapide de détermination du fibrinogène basée sur le temps de coagulation. Acta haematol 1957; 17: 237
- 8 Peterson E. A, Sober H. A. Chromatography of proteins. I. Cellulose ion-exchange adsorbents. J. Amer. Chem. Soc 1956; 78: 751
- 9 Sober H. A, Gutter F. J, Wijckoff M. M, Peterson E. A. Chromatography of proteins. II. Fractionations of serum protein on anion-exchange cellulose. J. Amer. Chem. Soc 1956; 78: 756
- 10 Fahey J. L, McCoy P. F, Goulian M. Chromatography of serum proteins in normal and pathologic sera: the distribution of protein-bound carbohydrate and cholesterol, siderophilin, thyroxin-binding protein, B12 binding protein, alkaline and acid phosphatases, radioiodinated albumin and myeloma proteins. J. Clin. Invest 1958; 37: 272
- 11 LOzner E. L, Kark R, Taylor F. H. L. The coagulation defect in haemophilia: the clot promoting activity in hemophilia of berkefelded normal human plasma free from fibrinogen and prothrombin. J. Clin. Invest 1939; 18: 603
- 12 Shinowara G. Enzyme studies on human blood XI. The isolation and characterization of thromboplastic cell and plasma components. J. Lab. Clin. Med 1951; 38: 11
- 13 Lorand L, Laki K. A simple method for purifying an activator of prothrombin. Biochim. biophys. Acta 1954; 13: 448
- 14 Wagner R. H, Richardson B. A, Brinkhous K. M. A study of the separation of fibrinogen and antihemophilic factor (AHF) in canine, porcine and human plasmas. Thromb. diath. haem 1957; 1: 1
- 15 Surgenor D. M, Steele B. B. 6th Annual Symposium on blood. January 1957. Thromb. diath. haem 1957; 1: 563
- 16 Soulier J. P, Gobbi F, Larrieu M. J. Séparation du fibrinogène et du facteur antihémophilique A. Rev. d’hématol 1957; 12: 481
- 17 Blombäck M. Purification of antihemophilic globulin I. Some studies on the stability of the antihemophilic globulin activity in fraction I-o and a method for its partial separation from fibrinogen.