Thromb Haemost 1969; 22(01): 151-163
DOI: 10.1055/s-0038-1651336
Originalarbeiten-Original Articles-Travaux Originaux
Schattauer GmbH

Some Properties of the Anticoagulant Factor of Aged Pooled Plasma[*]

G. R Honig**
1   Department of Pediatrics, University of Illinois College of Medicine Chicago, Illinois
,
Ch. F Abildgaard
1   Department of Pediatrics, University of Illinois College of Medicine Chicago, Illinois
,
E. N Forman
1   Department of Pediatrics, University of Illinois College of Medicine Chicago, Illinois
,
S. P Gotoff
1   Department of Pediatrics, University of Illinois College of Medicine Chicago, Illinois
,
Annabelle Lindley
1   Department of Pediatrics, University of Illinois College of Medicine Chicago, Illinois
,
I Schulman
1   Department of Pediatrics, University of Illinois College of Medicine Chicago, Illinois
› Author Affiliations
Supported by a graduate training grant (TI-AM-5344) from the National Institute of Arthritis and Metabolic Diseases, a grant (RG HE-07418) from the National Heart Institute, a grant (AM 10318) from the National Institute of Arthritis and Metabolic Diseases, and a grant (RG HD-00568) from the National Institute of Child Health and Human Development, National Institutes of Health, United States Public Health Service.
Further Information

Publication History

Publication Date:
28 August 2018 (online)

Summary

Hematologic studies of an infant who was admitted for shock and was treated with a large volume of pooled aged plasma disclosed striking prolongation of a variety of coagulation tests, including the prothrombin time, partial thromboplastin time, and thrombin time. The aged plasma produced a marked anticoagulant effect on normal plasma in vitro with the results suggesting inhibition of the thrombin-fibrinogen reaction. Similar results were obtained with 24 different lots of aged plasma from 5 different sources. The inhibitory factor of aged plasma behaved as a competitive inhibitor of the conversion of fibrinogen to fibrin, as compared with heparin which is a noncompetitive inhibitor. Infusion of aged plasma into rabbits produced a defect similar to that found in the patient. Biochemical and immunologic studies revealed that the plasma was devoid of fibrinogen but contained large amounts of a fibrinogen degradation product. Treatment of the plasmas with protamine or with rabbit IgG anti-human fibrinogen removed the anticoagulant activity. Development of anticoagulant activity during plasma aging did not appear to be related to fibrinolysis, as it was unaffected by addition of a fibrinolytic inhibitor. Because of the anticoagulant activity of aged plasma, it must be used with caution in patients having underlying hemostatic disorders. Coagulation screening tests in patients who have received recent infusions of aged plasma may lead to misinterpretation unless specific coagulation factor assays are performed.

* A preliminary report of these studies was presented at the thirtyeight annual meeting of the Society for Pediatric Research, May 4, 1968 at Atlantic City, New Jersey.


** Address: Department of Pediatrics, 840 South Wood Street, Chicago, Illinois, 60612.


 
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