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Thromb Haemost 1968; 19(01/02): 169-177
DOI: 10.1055/s-0038-1651193
Originalarbeiten – Original Articles – Travaux Originaux
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Theoretical Basis and Standardization of the One-Stage Lysis Time Method for Determination of Urokinase

W Berg
1   Medical Department II, Sahlgrenska Hospital, University of Göteborg, Sweden
,
K Korsan-Bengtsen
1   Medical Department II, Sahlgrenska Hospital, University of Göteborg, Sweden
,
J Ygge
1   Medical Department II, Sahlgrenska Hospital, University of Göteborg, Sweden
› Author Affiliations
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Publication History

Publication Date:
27 June 2018 (online)

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Summary

The theroretical basis for the use of a one-stage lysis time method for determination of urokinase is outlined. The calculations are based on experiments with purified plasminogen, fibrinogen, and thrombin preparations.

The following correlation between the lysis time and the urokinase, plasminogen, and fibrinogen concentrations was found :

This correlation is valid onyl for a certain range of plasminogen and urokinase concentrations. This validity has been verified experimentally.

A method has been proposed for determination of urokinase in which the curve of the standard graph always is rectilinear and has the slope –0.5. The method is very sensitive and its accuracy is great.

 

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