A Modified Two-Stage Assay Method for Tissue-Thromboplastin (TPLN)

N. R Kale

doi:10.1055/s-0038-1649441

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1966; 15(03/04): 403-412
DOI: 10.1055/s-0038-1649441

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

A Modified Two-Stage Assay Method for Tissue-Thromboplastin (TPLN)

N. R Kale^*

¹Chemistry Department II, Karolinska Institutet, Stockholm, Sweden

› Author Affiliations

Abstract

Summary

A two-stage method employing purified clotting factors for the assay of tissue-TPLN is described in detail. The rate of prothrombin activation is determined in the presence of optimum amounts of factors V, VII, X and Ca⁺⁺. The activation of prothrombin is arrested by addition of EDTA or STI, and the thrombin formed is assayed by the clotting method. The method is rapid, sensitive, reproducible and can differentiate between the tissue-TPLN activity of intact lipo-protein and the procoagulant activity of the phospholipids.

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References

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