Human Prothrombin: A New Method of Preparation from a Single Individual

Richard Benarous; Dominique Labie; François Josso

doi:10.1055/s-0038-1649121

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1973; 30(03): 425-436
DOI: 10.1055/s-0038-1649121

Original Article

Schattauer GmbH

Human Prothrombin: A New Method of Preparation from a Single Individual

Richard Benarous

¹Institut de Pathologie Moléculaire* 24, rue du Faubourg Saint-Jacques, 75014 Paris, France

²UER Necker, Dept of Hematology, 149, rue de Sèvres, 75007 Paris, France

,

Dominique Labie

¹Institut de Pathologie Moléculaire* 24, rue du Faubourg Saint-Jacques, 75014 Paris, France

²UER Necker, Dept of Hematology, 149, rue de Sèvres, 75007 Paris, France

,

François Josso

¹Institut de Pathologie Moléculaire* 24, rue du Faubourg Saint-Jacques, 75014 Paris, France

²UER Necker, Dept of Hematology, 149, rue de Sèvres, 75007 Paris, France

› Author Affiliations

Abstract

Summary

A simple method for purification of human prothrombin from ACT) plasma of one single individual is described. This method is based on three steps :

1. isolation of crude prothrombin complex by barium citrate adsorption;

2. first purification by hydroxyapatite chromatography;

3. further purification by DEAE Sephadex chromatography.

The final product was obtained with a good yield and exhibited less than 5 p. cent contamination. The human purified prothrombin obtained in this way displays :

– one single chain by SDS electrophoresis,

– a molecular weight of 75,000-80,000 daltons,

– a pi value pH 4.4 by electrofocusing,

– Alanine as थ terminal residue.

Amino-acid analysis is reported.

Full Text

References

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