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Thromb Haemost 1972; 28(03): 329-341
DOI: 10.1055/s-0038-1649016
Original Article
Schattauer GmbH

A New Method Based on One Dimensional Diffusion Using Small Glass Tubes for the Measurement of Fibrinolytic Activity

I. Fibrinolytic Activity of Plasmin and “Euglobulin + Streptokinase”
Taro Yasukouchi M. D.
1   Second Department of Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
,
Takeo Watanabe M. D.
1   Second Department of Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
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Further Information

Publication History

Publication Date:
29 June 2018 (online)

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Summary

A new method of estimating fibrinolytic activity is introduced. This method based on one dimensional diffusion (simple diffusion) is characterized by adopting small glass tubes, internal diameter about 3.5 mm calibrated from the bottom by 50 mm, as containers of substratecl fibrin.

The results and advantages over previous methods are as follows :

1. Clear line of demarcation between lyzed zone and remaining fibrin makes it easier to measure the extract of lysis than in the fibrin plate method.

2. The enzyme activity of materials tested is kept stable for many hours.

3. The quantity of test material in the fibrin tube is optional.

4. Only 1/10 fibrinogen required for fibrin plate method is sufficient.

5. Adopting a standard euglobulin unit gives a free choice of experimental conditions. The pooled euglobulin prepared from 48 healthy persons represented 1 standard euglobulin unit.

Standard deviations of “Euglobulin + Streptokinase” level in plasmas from 24 healthy men (aged 18-54 yrs) and women (aged 17-50 yrs) were ±0.27 and ±0.20 standard euglobulin units, respectively.

 

  • References

  • 1 Ambrus C. M, and Markus G. 1960; Plasmin-Antiplasmin Complex as a Reservoir of Fibrinolytic Enzyme. American Journal of Physiology 199: 491.
    PubMedGoogle Scholar
  • 2 Astrup T, Crookston J, and Macintyre A. 1950; Proteolytic Enzymes in Blood. Acta Physiologica Scandinavia 2: 238.
    PubMedGoogle Scholar
  • 3 Astrup T, and Mullertz S. 1952; The Fibrin Plate Method for Estimating Fibrinolytic Activity. Archives of Biochemistry and Biophysics 40: 346.
    CrossrefPubMedGoogle Scholar
  • 4 Christensen L. R. 1954; The Action of Proteolytic Enzymes on Casein Proteins. Archives of Biochemistry and Biophysics 53: 128.
    CrossrefPubMedGoogle Scholar
  • 5 Downie G. R, and Cliffton E. E. 1949; A Method for Assay of Serum Proteolytic Activity. Proceedings of the Society for Experimental Biology and Medicine 85: 142.
    PubMedGoogle Scholar
  • 6 Kaplan E. H. 1954; Action of Fibrinolysin (Plasmin) on Proteins. Proceedings of the Society for Experimental Biology and Medicine 85: 142.
    PubMedGoogle Scholar
  • 7 Kowalski M, Kopec M, and Niewarowski S. 1959; An Evaluation of Fibrinolysis. Journal of Clinical Pathology 12: 215.
    CrossrefPubMedGoogle Scholar
  • 8 Lassen M. 1953; Heat Denaturation of Plasminogen in the Fibrin Plate Method. Acta Physiologica Scandinavica 27: 371.
    PubMedGoogle Scholar
  • 9 Maki M, Saito H, and Yoneya T. 1964; Method of Antiplasmin Assay in Human Plasma. Tohoku Journal of Experimental Medicine 83: 168.
    CrossrefPubMedGoogle Scholar
  • 10 Markus G, and Ambeus C. M. 1960; On the Formation of Different Types of Plasmin by Streptokinase Activation. The Journal of Biological Chemistry 235: 1673.
    PubMedGoogle Scholar
  • 11 Milston H. 1941; A Factor in Normal Human Blood which participates in Streptococcal Fibrinolysis. Journal of Immunology 42: 109.
    PubMedGoogle Scholar
  • 12 Mullertz S, and Lassen M. 1953; An Activator System in Blood Indispensable for Formation of Plasmin by Streptokinase. Proceedings of the Society for Experimental Biology and Medicine 82: 264.
    PubMedGoogle Scholar
  • 13 Ouchteelony O. 1958. Diffusion-in-Gel Method for Immunological Analysis. In: Kallós P. (ed.) Progress in Allergy. Vol. 5. S. Karger AG; Basel & New York: 16.
    Google Scholar
  • 14 Permin P. M. 1947; Properties of the Fibrinokinase-Fibrinolysin System. Nature 169: 571.
    PubMedGoogle Scholar
  • 15 Permin P. M. 1950; Two Simple Methods of Determining Fibrinolytic Enzymes. Acta Physiologica Scandinavica 20: 388.
    CrossrefPubMedGoogle Scholar
  • 16 Ratnoff O. D. 1948; Some Factor Influencing the Enzymes Activated by Chloroform and by Streptococcal Fibrinolysin. The Journal of Experimental Medicine 87: 211.
    CrossrefPubMedGoogle Scholar
  • 17 Roberts P. S. 1958; Measurement of Rate of Plasmin Action on Synthetic Substrate. The Journal of Biological Chemistry 232: 285.
    PubMedGoogle Scholar
  • 18 Sherry S, Lindemeyer R. I, Fletcher A. P, and Alkjaersig N. 1959; Study on Enhanced Fibrinolytic Activity in Man. The Journal of Clinical Investigation 38: 810.
    CrossrefPubMedGoogle Scholar
  • 19 Torii T, Kawakami Y, and Kozima H. 1947; On an One Dimensional Diffusion Method of Assaying Penicillin and Other Antibiotic Substances. Journal of Penicillin 1: 281.
    PubMedGoogle Scholar
  • 20 Troll W. S, Sherry S, and Wachmann J. 1954; The Action of Plasmin on Synthetic Substrates. The Journal of Biological Chemistry 208: 85.
    PubMedGoogle Scholar
  • 21 Wolf P. 1968; Modification of the Fibrin Agar Plate for Measurements of the Components of the Fibrinolytic System. I. The Measurement of Plasminogen (on Type I fibrin agar plates). Thrombosis et Diathesis Haemorrhagica 20: 50.
    PubMedGoogle Scholar