Physico-Chemical Explanation of Blood Cell Adhesion in Thrombus Formation

Summary

A model is suggested which assumes that the rate of deposition of cells is determined both by hydrodynamic factors and by Brownian motion over the potential barrier caused by London and double-layer forces in the immediate vicinity of the deposition surface. The height of the barrier in the potential energy of interaction between blood cells and various surfaces is analyzed in relation to the physical properties of the cells, surfaces, and solutions. Based on this analysis, the adhesion of platelets to injured blood vessel walls and to non-biologic materials, the lack of adhesion of red blood cells under the same conditions, the mechanism of ADP induced aggregation and the interaction with blood flow are explained. The qualitative predictions of the model are substantiated by available experimental information. Quantitative results are presented in terms of a time constant, which typifies a period of contact with a surface, during which appreciable deposition occurs.

^* Present address: Department of Chemical Engineering, Technion, Haifa, Israel.

• References

• 1 Chandler A. B. 1971. The platelet in thrombus formation. In: Brinkhous K. M, Shermer R. W, Mostofi F. K. (Ed.) The Platelet. Ch. 12, The Williams & Wilkins Comp.; Baltimore.:
• 2 Dean P. M, Matthews E. K. 1975; The London-Van der Waals attraction constant of secretory granules and its significance. Journal of Theoretical Biology 54: 309.
• 3 Dutton R. C, Webber A. J, Johnson S. A, Baier R. F. 1969; Microstructure of initial thrombus formation on foreign materials. Journal of Biomedical Material Research 3: 13.
• 4 Goldsmith H. L. 1972; The flow of model particles and blood cells and its relation to thrombogenesis. In: Spaet T. H. (Ed.) Progress in Hemostasis and Thrombosis 1: 97.
• 5 Hamaker H. C. 1937; The London-Van der Waals attraction between spherical particles. Physica 4: 1058.
• 6 Hampton J. R, Mitchell J. R. A. 1966; Effect of aggregating agents on the electrophoretic mobility of human platelets. British Medical Journal 1: 1074.
• 7 Hogg R, Healy T. W, Fuerstenau D. W. 1966; Mutual coagulation of colloidal dispersions. Transactions of the Faraday Society 62: 1638.
• 8 Mason R. G, Gilkey J. M. 1971; A simple test for quantitation of platelet adhesion to glass. Studies in bleeder and non-bleeder subjects. Thrombosis et Diathesis Haemorrhagica 25: 21.
• 9 Mohandas N, Hochmuth R. M, Spaeth E. E. 1974; Adhesion of red cells to foreign surfaces in the presence of flow. Journal of Biomedical Research 8: 119.
• 10 Nir S. 1976 Van der Waals interaction between surfaces of biological interest. Progress in Surface Science. in press.
• 11 Ruckenstein E, Prieve D. C. 1975; Dynamics of cell deposition on surfaces. Journal of Theoretical Biology 51: 429.
• 12 Ruckenstein E, Marmur A, Rakower S. R. 1976; Sedimentation and adhesion of platelets onto a horizontal glass surface. Thrombosis and Haemostasis 36: 334.
• 13 Sawyer P. N, Srinivasan S. 1967; Study on the biophysics of intravascular thrombosis. The American Journal of Surgery 113: 42.
• 14 Seaman G. V. F. 1967; Surface potential and platelet aggregation. Thrombosis et Diathesis Haemor-rhagica Suppl 26: 53.
• 15 Seaman G. V. F, Vassar P. S, Kendall M. J. 1969; Calcium ion binding to blood cell surfaces. Experientia 25: 1259.
• 16 Thomas D. P. 1972. The role of platelets in arterial and venous thrombosis. In Mannucci P. M, Gorini S. (Ed.) Platelet function and thrombosis - A review of methods. Plenum Press; New York - London.:
• 17 Turitto V. T, Baumgartner H. R. 1974; Effect of temperature on platelet interaction with subendothelium exposed to flowing blood. Haemostasis 3: 224.
• 18 Weiss H. J. 1975; Platelet physiology and abnormalities of platelet function. The New England Journal of Medicine 293 (531) 580.
• 19 Weiss L. 1968; Studies on cellular adhesion in tissue culture. IX. Electrophoretic mobility and contact phenomena. Experimental Cellular Research 51: 609.
• 20 Weiss L, Harlos J. P. 1972; Some speculations on the rate of adhesion of cells to coverslips. Journal of Theoretical Biology 37: 169.
• 21 Wilkins D. J, Ottewill R. H, Bangham A. D. 1962; On the flocculation of sheep leucocytes: II. Stability studies. Journal of Theoretical Biology 2: 176.
• 22 Zucker M. B. 1972. Platelet function. In: Williams W. J. et al., (Ed.), Hematology. Ch. 122 McGraw-Hill.;
• 23 Zucker M. B, Vroman L. 1969; Platelet adhesion induced by fibrinogen adsorbed onto glass. Proceedings of the Society for Experimental Biology and Medicine 131: 318.