Inhibition of Human and Animal Platelet Adhesiveness to Glass Bead Columns by Adenosine, Dipyridamole, Chlorpromazine and Acetylsalicylic Acid

Summary

The differences among human, rabbit and guinea-pig platelet adhesiveness as for inhibitions by adenosine, dipyridamole, chlorpromazine and acetylsalicylic acid are described, and the influence of measurement conditions on platelet adhesiveness is also reported. Platelet adhesiveness of human and animal species decreased with an increase of heparin concentrations and an increase of flow rate of blood passing through a glass bead column. Human and rabbit platelet adhesiveness was inhibited in vitro by adenosine, dipyridamole and chlorpromazine, but not by acetylsalicylic acid. On the other hand, guinea-pig platelet adhesiveness was inhibited by the four drugs including acetylsalicylic acid. In in vivo study, adenosine, dipyridamole and chlorpromazine inhibited platelet adhesiveness in rabbits and guinea-pigs. Acetylsalicylic acid showed the inhibitory effect in guinea-pigs, but not in rabbits.

• References

• 1 Baumgartner H. J, Haudenschild C. 1972; Adhesion of platelets to subendothelium. Annals-of the New York Academy of Sciences 201: 22.
• 2 Born G. V. R, Cross M. J. 1964; Effects of inorganic ions and of plasma proteins on the aggregation of platelets by adenosine diphosphate. Journal of Physiology 170: 397.
• 3 Bowie E. J. W, Owen C. A, Thompson J. H, Didisheim P. 1969; Platelet adhesiveness in von Willebrand’s disease. American Journal of Clinical Pathology 52: 69.
• 4 Hattori A, Ito S, Tsukada T, Koike K, Matsuoka M. 1974; Platelet adhesion and aggregation in the glass bead column method (Hellem II): A scanning electron microscopic study. Archivum Histologicum Japonicum 36: 323.
• 5 Hellem A. J. 1960; The adhesiveness of human blood platelets in vitro. . Scandinavian Journal of Clinical and Laboratory Investigations 12 supplement 51.
• 6 Koike K. 1973; Experimental and clinical studies on platelet function. III. Effect of pyrimidopyrimidine derivatives (RA 233 and VK 744) on human platelet function in vitro and animal studies in the rabbit. The Japanese Journal of Clinical Hematology (In Japanese) 14: 1154.
• 7 O’Brien J. R. 1964; The adhesiveness of native platelets and its prevention. Journal of Clinical Pathology 14: 140.
• 8 Rossi E. C, Green D. 1972; A study of platelet retention by glass bead columns (‘platelet adhesiveness’) in normal subjects. British Journal of Haematology 23: 47.
• 9 Salzman E. W. 1963; Measurement of platelet adhesiveness. Journal of Laboratory and Clinical Medicine 62: 724.
• 10 Tsukada T, Ogawa T. 1973; On the mechanism of platelet retention by glass bead column. The platelet adhesiveness on glass bead column (Hellem II) was studied by scanning electron microscopy. The Japanese Journal of Clinical Hematology (In Japanese) 14: 777.
• 11 Weiss H. J, Aledort L. M. 1967; Impaired platelet/connective tissue reaction in man after aspirin ingestion. Lancet 2: 495.