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Thromb Haemost 1976; 36(02): 392-400
DOI: 10.1055/s-0038-1648054
Original Article
Schattauer GmbH

Effect of Platelet Age on Adhesiveness to Collagen and Platelet Surface Charge

Robert M. Castellan
1   Division of Hematologic Research, The Memorial Hospital, Pawtucket and Brown University, Providence, R.I., U.S.A.
,
Manfred Steiner
1   Division of Hematologic Research, The Memorial Hospital, Pawtucket and Brown University, Providence, R.I., U.S.A.
› Author Affiliations
Further Information

Publication History

Received 20 February 1976

Accepted 02 June 1976

Publication Date:
03 July 2018 (online)

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Summary

Adhesion to collagen was investigated as a function of platelet age in rat platelets. Platelet adherence was measured using EDTA-containing platelet- rich plasma which was added to preparations of collagen fibers clamped between magnetic stirrers by recording changes in light transmission. The plot of light transmission versus logarithm of time was linear and allowed calculation of a slope factor which related to the rate of adherence. Neither the amount of collagen nor the platelet count were limiting in the test. Young platelet populations (≤ 1 day old) were obtained during the recovery phase from immune induced thrombocytopenia. Old platelet populations were prepared by blocking thrombopoiesis with cyclophosphamide. Young platelets showed a moderate but statistically significant increase in adhesivity to collagen but old platelets did not differ significantly from randomly aged platelets in this function. The electrophoretic mobility of platelets was not affected by their age.

 

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