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Thromb Haemost 1976; 35(03): 576-585
DOI: 10.1055/s-0038-1648000
DOI: 10.1055/s-0038-1648000
Original Article
Purification and Properties of Human Factor IXa
Further Information
Publication History
Received:
23 March 1975
Accepted:
06 September 1975
Publication Date:
02 July 2018 (online)
Summary
Human factor IXa was purified 5,000-fold from serum by ion exchange chromatography. The preparation was free from other clotting factors. Both pH sensitivity and heat stability of purified factor IXa appeared to be different from those of factor IX in the plasma. The molecular weight of human factor IXa is 80,000 as estimated from gel-filtration experiments. Modification of seryl or histidyl side chains abolished the activity of factor IXa.
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