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Thromb Haemost 1976; 35(02): 274-288
DOI: 10.1055/s-0038-1647956
Original Article
Schattauer GmbH

The Estimation of the Number of Binding Sites for Antibody on Antigen Molecules with Reference to Factor VIII and its Antibody

Judith Pool
1   Oxford Haemophilia Centre, Churchill Hospital, Headington, Oxford, OX3 7LJ and the Department of Medicine, Stanford University Medical Center, Stanford, California 94305, U.S.A.
,
Rosemary Biggs
1   Oxford Haemophilia Centre, Churchill Hospital, Headington, Oxford, OX3 7LJ and the Department of Medicine, Stanford University Medical Center, Stanford, California 94305, U.S.A.
,
R. G Miller
1   Oxford Haemophilia Centre, Churchill Hospital, Headington, Oxford, OX3 7LJ and the Department of Medicine, Stanford University Medical Center, Stanford, California 94305, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 05 June 1975

accepted 20 July 1975

Publication Date:
02 July 2018 (online)

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Summary

The theoretical basis for determining the number of antibody sites on antigen molecules is examined. The theoretical considerations are applied to factor VIII molecules. Examples based on data available at the Oxford Haemophilia Centre are calculated to illustrate the approach. It is concluded that there are few sites on each factor VIII molecule for human antibody. The three antibodies for which reasonable data were available suggest 1–3 sites for human antibody. The data for rabbit antibody suggest 5–6 sites per factor VIII molecule.

 

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