Download PDF
Thromb Haemost 1976; 35(02): 342-349
DOI: 10.1055/s-0038-1647927
Original Article
Schattauer GmbH

The Young Platelet Population in Children with Cyanotic Congenital Heart Disease

Béla Goldschmidt
1   Second Department of Paediatrics, Semmelweis Medical University, Budapest, Hungary, Institute for Thrombosis Research and Department of Paediatrics, Rikshospitalet, Oslo, Norway
,
Svein Jan Sørland
1   Second Department of Paediatrics, Semmelweis Medical University, Budapest, Hungary, Institute for Thrombosis Research and Department of Paediatrics, Rikshospitalet, Oslo, Norway
› Author Affiliations
Further Information

Publication History

Received 04 August 1975

Accepted 20 October 1975

Publication Date:
02 July 2018 (online)

    Permissions and Reprints

Summary

Some morphological, biochemical and functional parameters of platelet population in children with cyanotic congenital heart disease (CCHD) were studied by making comparisons of the normal platelet population in both CCHD patients and controls. The mean volume of the platelets from cyanotic patients was greater than from normals. The platelet size distribution curves demonstrated a shift towards larger than normal size in the case of CCHD. The mean protein content, as well as the mean PF3 content of platelets was increased in CCHD. Following addition of kaolin, PF3 release was more rapid and of shorter duration with platelets from CCHD patients as compared to normal platelets. They also released more PF3 than did normal platelets. After addition of ADP, collagen, or adrenalin, platelets of CCHD patients were more responsive than similarly treated platelets from normals. Platelets from CCHD showed an increased initial rate of aggregation and greater maximum aggregation. These data suggested that the platelet population of CCHD patients consists of larger, younger and functionally more active platelets than does the platelet population of normals.

 

  • References

  • 1 Born G. V. R. 1962; Quantitative investigations into the aggregation of blood platelets. Journal of Physiology 762: 67.
    PubMedGoogle Scholar
  • 2 Bøyum A. 1962; Separation of leukocytes from blood and bone marrow. Scandinavian Journal of Clinical and Laboratory Investigation, Supplement 21: 97.
    PubMedGoogle Scholar
  • 3 Detwiler T. C, Odell Jr. T. T, McDonald T. P. 1962; Platelet size, ATP content and clot retraction in relation to platelet age. American Journal of Physiology 203: 107.
    PubMedGoogle Scholar
  • 4 Feissly R, Lüdin H. 1949; Microscopie par contrastes de phases; applications a l’hématologie. Revue d’hématologie 4: 481.
    PubMedGoogle Scholar
  • 5 Ginsburg A. D, Aster R. H. 1970; Changes associated with platelet aging. Thrombosis et Diathesis Haemorrhagica 23: 407.
    PubMedGoogle Scholar
  • 6 Goldschmidt B. 1970; Blutkoagulationsstörungen bei Kindern mit angeborenen Herzfehlern. Monatsschrift fur Kinderheilkunde 118: 450.
    PubMedGoogle Scholar
  • 7 Goldschmidt B, Fonó R. 1972; Cyclic fluctuations in platelet count, megakaryocyte maturation and thrombopoietin activity in cyanotic congenital heart disease. Acta Paediatrica Scandinavica 61: 310.
    CrossrefPubMedGoogle Scholar
  • 8 Goldschmidt B, Sarkadi B, Gárdos G, Matlary A. 1974; Platelet production and survival in cyanotic congenital heart disease. Scandinavian Journal of Haematology 13: 110.
    PubMedGoogle Scholar
  • 9 Grossman C. M, Kohn R. 1965; Enzymatic characteristics of in vitro incorporation of P32 orthophosphate into human platelet phosphatides. Thrombosis et Diathesis Haemorrhagica 13: 126.
    PubMedGoogle Scholar
  • 10 Holmsen H, Ostvold A. C, Day H. J. 1973; Behaviour of endogenous and newly absorbed serotonin in the platelet release reaction. Biochemical Pharmacology 22: 2599.
    CrossrefPubMedGoogle Scholar
  • 11 Ihenacho H. N. C, Breeze G. P, Fletcher G. J, Stuart J. 1973; Consumption coagulopathy in congenital heart disease. Lancet 1: 231.
    PubMedGoogle Scholar
  • 12 Inceman S, Tangün Y. 1969; A simple test for the evaluation of platelet coagulant activity. Journal of Laboratory and Clinical Medicine 74: 174.
    PubMedGoogle Scholar
  • 13 Karpatkin S. 1969; Heterogenity of human platelets II. Functional evidence suggestive of young and old platelets. Journal of Clinical Investigations 48: 1083.
    PubMedGoogle Scholar
  • 14 Karpatkin S. 1972; Human platelet senescence. Annual Review of Medicine 23: 101.
    CrossrefPubMedGoogle Scholar
  • 15 Lövry O. H, Rosenbrough N. J, Farr A. L, Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193: 265.
    PubMedGoogle Scholar
  • 16 Maurer H. M, McCue C. M, Caul J, Still W. J. S. 1972; Impairement in platelet aggregation in cyanotic congenital heart disease. Blood 40: 207.
    PubMedGoogle Scholar
  • 17 McDonald T. P, Odell Jr. T. T, Gosslee D. G. 1964; Platelet size in relation to platelet age. Proceedings of the Society for experimental Biology and Medicine 115: 684.
    PubMedGoogle Scholar
  • 18 Miller G. L. 1959; Protein determinations for large numbers of samples. Analytical Chemistry 31: 964.
    PubMedGoogle Scholar
  • 19 O’Brien J. R. 1973. Drug induced thrombocytopathy. In: Girdwood R. H. (ed.) Blood disorders due to drugs and other agents . Excerpta Medica Foundation; Amsterdam:
    Google Scholar
  • 20 Spaet T. H, Cintron J. 1965; Studies on platelet factor 3 availability. British Journal of Haematology 11: 269.
    CrossrefPubMedGoogle Scholar
  • 21 Stormorken H, Lund-Ruse A, Røvik T. O. 1965; Platelet adhesiveness to glass beads: methodological investigations using automatic platelet counting. Scandinavian Journal of Clinical and Laboratory Investigation. Supplement 85: 184.
    PubMedGoogle Scholar