Thromb Haemost 1976; 35(01): 082-086
DOI: 10.1055/s-0038-1647913
Original Article
Schattauer GmbH

Isolation and Preliminary Characterization of a Vitamin K Dependent Peptide from Human Prothrombin[*]

J Elion
1   Institut de Pathologie Moléculaire**, 24, rue du Faubourg Saint-Jacques, Paris 14e, France.
,
R Benarous
1   Institut de Pathologie Moléculaire**, 24, rue du Faubourg Saint-Jacques, Paris 14e, France.
,
D Labie
1   Institut de Pathologie Moléculaire**, 24, rue du Faubourg Saint-Jacques, Paris 14e, France.
› Author Affiliations
Further Information

Publication History

Received: 29 September 1975

accepted: 29 September 1975

Publication Date:
02 July 2018 (online)

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Summary

A peptide containing the vitamin K dependent Ca2+ binding region of human prothrombin has been isolated by adsorption of the tryptic digest of the native protein on barium citrate. Its aminoacid composition has been determined. The N- and C-terminal residues have been characterized. The obtained results are showing some slight differences with the corresponding bovine peptide. Since they were not strictly identical for two different preparations, the eventuality of a molecular heterogeneity is discussed.

* Presented at the Vth Congress of the International Society on Thrombosis and Haemostasis, Paris, July, 1975.

* ** Groupe U 15 de l’Institut National de la Santé et de la Recherche Médicale, Laboratoire Associé au Centre National de la Recherche Scientifique.