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Thromb Haemost 1974; 32(02/03): 678-684
DOI: 10.1055/s-0038-1647737
Original Article
Schattauer GmbH

Evidence for Ca2+ Regulated Magnesium ATPase of Thrombosthenin (Platelet Actomyosin)

C Mahendran   *
1   Department of Biochemistry, Downstate Medical Center, State University of New York, Brooklyn, New York, U.S.A
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Further Information

Publication History

Received 04 March 1974

Accepted 06 August 1974

Publication Date:
30 June 2018 (online)

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Summary

The isolation of an actomyosin-like protein from platelets with a Ca2+ sensitive component is described. The addition of 1 mM EGTA results in approximately 40% and 75% inhibition of the Mg-ATPase activity depending on the mode of isolation. The inhibition can be released by a free Ca2+ concentration of 3×l0-6 M. Superprecipitation is also inhibited by 1 mM EGTA and restored in the presence of 10-5 M free Ca2+.

* Present Address: Department of Neurology Mount Sinai School of Medicine of the City Univ. of New York Fifth Avenue and 100th street New York, New York 10029, U.S.A.


 

  • References

  • 1 Bettex-Galland M, Lüscher E. F. 1961; Thrombosthenin: A contractile protein from thrombocyte. Its extraction from human blood platelets and some of its properties. Biochimica Biophysica Acta 49: 536.
    PubMedGoogle Scholar
  • 2 Bettex-Galland M, Lüscher E. F, Weibel E. R. 1969; Thrombosthenin- electron microscopical studies on its localization in human blood platelets and some properties of its subunits. Thrombosis et Diathesis Haemorrhagica 22: 431.
    PubMedGoogle Scholar
  • 3 Carsten M. 1971; Uterine smooth muscle: Troponin. Archives of Biochemistry and Biophysics 147: 353.
    CrossrefPubMedGoogle Scholar
  • 4 Cohen I. 1971 A tropomyosin-like protein from human blood platelets. Federation Proceedings. 1309.
    PubMedGoogle Scholar
  • 5 Cohen I, Cohen C. 1972; A tropomyosin-like protein from human platelets. Journal of Molecular Biology 68: 383.
    CrossrefPubMedGoogle Scholar
  • 6 Dow J, Stracher A. 1971; Changes in the properties of myosin associated with muscle development. Biochemistry 10: 1316.
    CrossrefPubMedGoogle Scholar
  • 7 Ganguly P. 1969; Studies on platelet proteins. IV. Some physical and chemical properties of thrombosthenin. Blood 34: 511.
    PubMedGoogle Scholar
  • 8 Guette K. 1969; Relaxing factor in extracts of blood platelets and its function in the cells. Nature 198: 488.
    PubMedGoogle Scholar
  • 9 Hanson J. P, Repke D. I, Katz A. M, Aledort L. M. 1972 A troponintropomyosin-like Ca2+ sensitizing system in human platelets. IIIrd Congress of International Society on Thrombosis and Hemostasis. Abstract 200.
    PubMedGoogle Scholar
  • 10 Haslam R. J, Mustard J. F. 1966 Factors affecting the superprecipitation of pig thrombostenin. Fourth Annual Conference on Blood Platelets. Oak Ridge, Tenn. 13.
    PubMedGoogle Scholar
  • 11 Lowry O. H, Rosebrough N. J, Farr A. L, Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193: 265.
    PubMedGoogle Scholar
  • 12 Mahendran C, Nickxas W. J, Berl S. 1974 In press.
    PubMed
  • 13 Nachman R. L, Marcus A. J, Safier L. B. 1967; Platelet thrombosthenin: Subcellular localization and function. Journal of Clinical Investigation 46: 1380.
    CrossrefPubMedGoogle Scholar