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Thromb Haemost 1974; 32(02/03): 432-440
DOI: 10.1055/s-0038-1647711
Original Article
Schattauer GmbH

A Method for Blood Platelet Homogenization Using the Aminco-French Pressure Cell

Paul C. French
1   Institute for Thrombosis Research, Rikshospitalet, and Electron Microscopic Laboratory, Rikshospitalet, Oslo 1, Norway
,
Randi Holme
1   Institute for Thrombosis Research, Rikshospitalet, and Electron Microscopic Laboratory, Rikshospitalet, Oslo 1, Norway
› Author Affiliations
Further Information

Publication History

Received 12 February 1974

Accepted after resubmission 20 June 1974

Publication Date:
24 July 2018 (online)

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Summary

A rapid effective method for platelet homogenization using a pressure cell is described. With this method, almost complete homogenization of human platelets occurred without excessive solubilization of enzymes as compared to a pestle homogenization procedure where there was incomplete homogenization. Electron microscopy of pressure homogenates and fractions revealed well preserved organelles.

 

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