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Thromb Haemost 1974; 32(01): 232-238
DOI: 10.1055/s-0038-1647688
Original Article
Schattauer GmbH

Electronic Platelet Counting after Isopycnic Differential Centrifugation A Simple Rapid and Accurate Method

M De Bruyere
1   Department of Haematology of the University of Louvain ( Belgium)
,
CL Bellenot
1   Department of Haematology of the University of Louvain ( Belgium)
,
G Sokal
1   Department of Haematology of the University of Louvain ( Belgium)
› Author Affiliations
Further Information

Publication History

Received 15 February 1974

Accepted after resubmission 09 May 1974

Publication Date:
30 June 2018 (online)

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Summary

A semi-automatized platelet counting procedure with the use of a Coulter Counter is described. Its originality consists in providing an electronic method of counting of the platelets after a differential centrifugation of the blood sample previously diluted with a hypertonic NaCl solution (density 1.053) which is isopycnic to platelet density. This procedure allows elimination of red and white cells without loss of platelets in the supernatant. The final platelet number can be calculated without further corrections.

The method is quick and does not require specially skilled technicians. A single technician can perform 40 counts in one hour. The reproductibility of the method is excellent and the coefficient of variation 2,4 %.

The accuracy and feasibility of the method is also sustained by two years’ experience in an haematological routine laboratory.

 

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