CC BY-NC-ND 4.0 · Laryngorhinootologie 2018; 97(S 02): S114-S115
DOI: 10.1055/s-0038-1640108
Poster
Onkologie: Oncology

Isolation of adult stem cells from the nasopharynx

A Münscher
1   HNO Universitätsklinik Hamburg-Eppendorf, Hamburg
,
W Ching
2   Heinrich Pette Institut, Leibniz Institut für experimentelle Virologie, Hamburg
,
E Pfrommer
3   Heinrich Pette Institut, Leibniz Institut für Exerimentelle Virologie, Hamburg
,
R Reimer
2   Heinrich Pette Institut, Leibniz Institut für experimentelle Virologie, Hamburg
,
C Dreier
2   Heinrich Pette Institut, Leibniz Institut für experimentelle Virologie, Hamburg
,
B Wilkens
2   Heinrich Pette Institut, Leibniz Institut für experimentelle Virologie, Hamburg
,
G Pilnitz-Stolze
2   Heinrich Pette Institut, Leibniz Institut für experimentelle Virologie, Hamburg
,
N Möckelmann
4   HNO Universitätsklinik Hamburg Eppendorf, Hamburg
,
G Gabriel
5   Heinrich Pette Institu, Leibniz Institut für experimentelle Virologie, Hamburg
,
T Dobner
6   Heinrich Pette Institut, Leiniz Institut für experimentelle Virologie, Hamburg
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    Introduction:To date, modelling disease mechanisms in the human nasopharynx is limited to the usage of difficult-to-obtain ex vivo organ cultures, quantity-limited primary nasopharynx cell culture models or immortalized nasopharyngeal tumour cell lines. Each system harbours specific advantages but also disadvantages and only the human ex vivo organ system can sufficiently recapitulate tissue architecture and also infection mechanisms. However, limitations occur in the availability and reproducibility of the material and the long-term usage of the tissue.

    During the last decade, adult stem cells have become important tools in biomedical research to mimic human (and mouse) organ architecture in vitro. These cells can be grown and expanded in 3D cultures as organoids or on fibroblasts in the ground-state manifold. Adult stem cells have been isolated from many different organs or tissues like liver, pancreas, stomach, intestine, skin, kidney and lung. These in vitro grown mini organs are used to understand different infectious diseases or cancer.

    Methods:

    The tissue stem cells can be expanded manifold, cryopreserved and reused after thawing. By using the air-liquid interface differentiation method we could achieve organotypic differentiation into nasopharynx-like tissue.

    Results:

    Here, we describe the isolation, long-term cultivation and characterization of epithelial stem cells (adult stem cells) from human nasopharynx tissue.

    Conclusion:

    This achievment will be opening up possibilities for direct infection analyses with pathogenic agents having nasopharynx tissue tropism.


    #

    No conflict of interest has been declared by the author(s).

    PD Dr. med. Adrian Münscher
    HNO Universitätsklinik Hamburg-Eppendorf,
    Martinistraße 52, 20246,
    Hamburg

    Publication History

    Publication Date:
    18 April 2018 (online)

    © 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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