Development of an Animal Model Using a Closed System to Study the Sensitivity of a Radiopharmaceutical for the Detection of Gastrointestinal Bleeding

A. Owunwanne; K. K. Mahajan; H. M. Abdel-Dayem; S. B. S. Ericksson; S. Sadek; T. Yacoub; M. Awdeh

doi:10.1055/s-0038-1628875

Nuklearmedizin - NuclearMedicine, Table of Contents

Nuklearmedizin 1987; 26(03): 126-130
DOI: 10.1055/s-0038-1628875

Review Articles

Schattauer GmbH

Development of an Animal Model Using a Closed System to Study the Sensitivity of a Radiopharmaceutical for the Detection of Gastrointestinal Bleeding

A. Owunwanne

^*From the Departments of Nuclear Medicine and of Surgery

,

K. K. Mahajan

^*From the Departments of Nuclear Medicine and of Surgery

,

H. M. Abdel-Dayem

^*From the Departments of Nuclear Medicine and of Surgery

,

S. B. S. Ericksson

^*From the Departments of Nuclear Medicine and of Surgery

,

S. Sadek

^*From the Departments of Nuclear Medicine and of Surgery

,

T. Yacoub

^*From the Departments of Nuclear Medicine and of Surgery

,

M. Awdeh

^*From the Departments of Nuclear Medicine and of Surgery

› Author Affiliations

Abstract

An experimental animal model using a closed system to study the sensitivity of radiopharmaceuticals for the detection and localization of gastrointestinal (Gl) bleeding site in a sheep was developed. This model was validated with ^99mTc-DTPA. Radioactivity as low as 85.47 ± 13.32 kBq in a volume of 2.1 ±0.14 ml at a bleeding rate of 0.07 ml/min was detected. Simulated intermittent bleeding experiments indicated that at 1 h after injection of ^99mTc-DTPA there was still enough circulating radioactivity to bleed into the gut and that it was possible to perform repeat injection of ^99mTc-DTPA as early as 2 h after the first injection.

Zusammenfassung

Ein experimentelles Tiermodell (geschlossenes System) wurde entwik-kelt, das die Sensitivität einzelner Radiopharmazeutika in der Entdeckung und Lokalisation gastrointestinaler Blutungen ermöglicht. Dieses Modell wurde am Schaf mit ^99mTc-DTPA erprobt. Hiermit konnten Blutungsmengen von 0,07 ml/min (85,47 ± 13,32 kBq/2,1 ± 0,14 ml) entdeckt werden. Die Simulation einer intermittierenden Blutung ergab, daß eine Stunde nach der Injektion von ^99mTc-DTPA ausreichende Radioaktivitätsmengen im Blut zirkulieren, um auch dann Blutungen nachzuweisen. Auch die Reinjektion des Tracers nach zwei Stunden erwies sich als brauchbarer Weg.

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References

REFERENCES
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