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Nuklearmedizin 1981; 20(06): 299-304
DOI: 10.1055/s-0037-1620663
Originalarbeiten – Original Articles
Schattauer GmbH

99mTc-Labelled Cu(I)-EHDP, a Potential Skeletal Imaging Agent

99mTc-markiertes Cu(I)-EHDP, ein potentielles Agens für die Skelettszintigraphie
N.H. Agha
1   From the Nuclear Research Centre, Radioisotope Production Department, Tuwaitha, Baghdad, Iraq
,
A.M. Al-Hilli
1   From the Nuclear Research Centre, Radioisotope Production Department, Tuwaitha, Baghdad, Iraq
,
H.A. Hassen
1   From the Nuclear Research Centre, Radioisotope Production Department, Tuwaitha, Baghdad, Iraq
› Author Affiliations
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Publication History

Received:25 February 1981

Publication Date:
09 January 2018 (online)

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99mTc-Cu-EHDP has been prepared with high labelling yield applying for the first time the method of instantly formed cuprous ions in the mixture. A gelchromatography column scanning technique has been used to study the 99mTc fractions in the preparation. The study of the influence of pH-value on the amount of 99mTc-Cu-EHDP fraction shows that pH 1.6 - 1.7 gave the best labelling results. The formation rate of 99mTc-Cu-EHDP complex with a high labelling yield was fast and achieved within a few mins. This suggests the reduction of 99mTc-pertechnetate to Tc (IV). The final preparation was found stable for at least 4 hrs after mixing the reactants with the 99mTc-eluate. Comparative biokinetic studies of 99mTc-Cu-EHDP and 99mTc-Sn-EHDP in rabbits and mice showed a high bone uptake and fast elimination of 99mTc-Cu-EHDP from the skeleton. No significant difference was found in the plasma protein binding of 99mTc-Cu-EHDP and 99mTc-Sn-EHDP in rats as assessed by the GCS-technique. Radionuclide imaging in rabbits, using a gamma camera, showed 99mTc-Cu-EHDP to be a good bone-imaging agent.

Mit hoher Ausbeute gelang es erstmals, 99mTc Cu(I)-EHDP unter Anwendung einer Methode zur schnellen Bildung von Kupferionen in Mischlösungen herzustellen. Es wurde eine Gel-Chromatographiesäule verwendet, um die Technetium-fraktionen in der Präparation zu untersuchen. Bei einem pH zwischen 1,6 und 1,7 ergab sich die höchste Menge der 99mTc Cu(I)-EHDP-Fraktion. Eine hohe Ausbeute des Komplexes konnte in wenigen Minuten erzielt werden. Dies läßt die Vermutung zu, daß 99mTc-Pertechnetat zu Tc(IV) reduziert wird. Das Endprodukt war nach Mischen der Reagenzlösungen mit dem 99mTc-Eluat für wenigstens 4 Std. stabil. Vergleichende kinetische Untersuchungen mit 99mTc-Cu-EHDP und 99mTc-Sn-EHDP bei Kaninchen und Mäusen zeigte eine hohe Knochenaufnahme und eine schnelle Elimination des 99mTc-Cu-EHDP-Komplexes aus dem Skelett. Keine signifikanten Unterschiede wurden für die Plasmaproteinbindung zwischen dem Cu- und dem Sn-Komplex bei Ratten unter Verwendung der GCS-Technik gefunden. Die Abbildung von 99mTc-Cu-EHDP bei Kaninchen mit einer Gamma-Kamera zeigte, daß dieser Komplex ein gutes Radiopharmakon für die Abbildung von Knochen ist.

 

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