Subscribe to RSS
Please copy the URL and add it into your RSS Feed Reader.
https://www.thieme-connect.de/rss/thieme/en/10.1055-s-00035024.xml
Download PDF
Thromb Haemost 2001; 86(06): 1555-1562
DOI: 10.1055/s-0037-1616762
DOI: 10.1055/s-0037-1616762
Review Article
Extracellular Signal-regulated Kinase (ERK) Activation Is Required for GP Ibα-dependent Endothelial Cell Migration[*]
This work was supported in part by the National Institutes of Health R29HL-51415 (DAB), the National American Heart Association 96011230 (DAB), PA/DE Affiliate of the American Heart Association 440515-431 (SN) and an Initial Investigator Award (CM).
Further Information
Publication History
Received
12 February 2001
Accepted after resubmission
07 August 2001
Publication Date:
12 December 2017 (online)
Summary
The GP Ib complex can participate in endothelial cell (EC) migration on von Willebrand factor (vWF) or the mixed matrix of vWF and type I collagen (vWF/collagen). In this study, viper venom proteins alboaggregin (albo) A or B blocked GP Ibα, and echistatin inhibited αvβ3 binding. Albo A, B and echistatin inhibited EC migration on vWF and vWF/collagen. Albo B or the anti-GP Ibα monoclonal antibody (mAb) 1b1 did not affect the migration of smooth muscle cells or fibroblasts, which lack GP Ib. EC also migrate on albo A- or albo B-coated dishes. PD98059, which blocks ERK activation, abolished EC migration on vWF, vWF/collagen, collagen or albo B. Soluble albo A or 1b1 dramatically inhibited ERK activation during EC migration on vWF or albo B. Echistatin inhibited ERK activation on vWF and vitronectin (VN), but not albo B. Thus, in addition to αvβ3, EC GP Ibα initiates ERK activation, and regulates ERK-induced EC migration on vWF.
* Dedicated to Stefan Niewiarowski, MD, PhD, 1926-2001.
-
References
- 1 Lauffenburger DA, Horwitz AF. Cell migration: A physically integrated molecular process. Cell 1996; 84: 359-69.
- 2 Byzova TV, Rabbani R, D’Souza SE, Plow EF. Role of integrin αvβ3 in vascular biology (Review). Thromb Haemost 1998; 80: 726-34.
- 3 Shuster CB, Herman IM. The mechanics of vascular cell motility. Microcirculation 1998; 5: 239-57.
- 4 Cheresh DA, Berliner SS, Vicente V, Ruggeri ZM. Recognition of distinct adhesive sites on fibrinogen by related integrins on platelets and endothelial cells. Cell 1989; 58: 945-53.
- 5 Leavesley DI, Ferguson GD, Wayner EA, Cheresh DA. Requirement of the integrin β3 subunit for carcinoma cell spreading or migration on vitronectin and fibrinogen. J Cell Biol 1992; 117: 1101-7.
- 6 Leavesley DI, Schwartz MA, Rosenfeld M, Cheresh DA. Integrin β1-and β3-mediated endothelial cell migration is triggered through distinct signaling mechanisms. J Cell Biol 1993; 121: 163-70.
- 7 Brooks PC, Clark RAF, Cheresh DA. Requirement of vascular integrin αvβ3 for angiogenesis. Science 1994; 264: 569-71.
- 8 Aplin AE, Howe AK, Alahari SK, Juliano RL. Signal transduction and signal modulation by cell adhesion receptors: the role of integrins, immunoglobulin-cell adhesion molecules, and selectins. Pharmacol Rev 1998; 50: 197-263.
- 9 Eliceiri BP, Klemke R, Stromblad S, Cheresh DA. Integrin αvβ3 requirement for sustained mitogen-activated protein kinase activity during angiogenesis. J Cell Biol 1998; 140: 1255-63.
- 10 Lian J, Guoping C, Tran L-P, Shapiro SS, Beacham DA. Glycoprotein Ib can mediate endothelial cell migration on vWF-containing substrata. Expt’l Cell Res 1999; 252: 114-22.
- 11 Xia Z, Dickens M, Raingeaud J, Davis R. Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science 1995; 270: 1326-31.
- 12 Klemke RL, Cai S, Giannini AL, Gallagher PJ, Lanerolle PD, Cheresh DA. Regulation of cell motility by mitogen-activated protein kinase. J. Cell Biol 1997; 137: 481-92.
- 13 Wu G, Essex DW, Meloni FJ, Takafuta T, Fujimura K, Konkle BA, Shapiro SS. Human endothelial cells in culture and in vivo express on their surface all four components of the glycoprotein Ib/IX/V complex. Blood 1997; 90: 2660-9.
- 14 Beacham DA, Cruz MA, Handin RI. Glycoprotein Ib can mediate endothelial cell attachment to a von Willebrand Factor substratum. Thromb Haemost 1995; 73: 309-17.
- 15 Beacham DA, Tran L-P, Shapiro SS. Cytokine treatment of endothelial cells increases glycoprotein Ibα-dependent adhesion to von Willebrand Factor. Blood 1997; 89: 4071-7.
- 16 Kroll MH, Harris TS, Moake JL, Handin RI, Schafer AI. Von Willebrand factor binding to platelet GP Ib initiates signals for platelet activation. J Clin Invest 1991; 88: 1568-73.
- 17 López JA. The platelet glycoprotein Ib-IX complex. Blood Coag Fibrinol 1994; 5: 97-119.
- 18 Tan L, Kowalski MA, Romo GM, Lopez JA, Darzynkiewicz Z, Niewiarowski S. Identification and characterization of endothelial glycoprotein Ib using viper venom proteins modulating cell adhesion. Blood 1999; 93: 2605-16.
- 19 Beacham DA, Wise RJ, Turci SM, Handin RI. Selective inactivation of the Arg-gly-asp-ser (RGDS) binding site in von Willebrand Factor by site-directed mutagenesis. J Biol Chem 1992; 267: 3409-15.
- 20 Peng M, Lu W, Kirby EP. Characterization of three alboaggregins purified from Trimeresurus albolabris venom. Thromb Haemost 1992; 67: 702.
- 21 Marcinkiewicz C, Lobb RR, Marciniewicz MM, Daniel JL, Smith JB, Dangelmaier C, Weinreb PH, Beacham DA, Niewiarowski S. Isolation and characterization of EMS16, a C-lectin type protein from Echis multi-squamatus venom, a potent and selective inhibitor of the α2β1 integrin. Biochemistry 2000; 39: 9859-67.
- 22 Marcinkiewicz C, Calvete JJ, Vijay-Kumar S, Marcinkiewicz MM, Raida M, Schick P, Lobb RR, Niewiarowski S. Structural and functional characterization of EMF10, a heterodimeric disintegrin from Eristocophis macmahoni venom that selectively inhibits alpha 5 beta 1 integrin. Biochemistry 1999; 38: 13302-9.
- 23 Cai G, Lian J, Shapiro SS, Beacham DA. Evaluation of endothelial cell migration with a novel in vitro assay system. Methods in Cell Science 2000; 22: 107-14.
- 24 DeMarco L, Mazzucato M, Masotti A, Fenton JW, Ruggeri ZM. Function of glycoprotein Ibα in platelet activation induced by α-thrombin. J Biol Chem 1991; 266: 23776-83.
- 25 Chen Q, Kinch MS, Lin TH, Burridge K, Juliano RL. Integrin-mediated cell adhesion activates mitogen-activated protein kinases. J Biol Chem 1994; 269: 26602-5.
- 26 McLane MA, Marcinkiewicz C, Vijay-Kumar S, Wierzbicka-Patynowski I, Niewiarowski S. Viper venom disintegrins and related molecules. Proc Soc Exptl Biol Med 1998; 219: 109-19.
- 27 Klemke RL, Yebra M, Bayne EM, Cheresh DA. Receptor tyrosine kinase signaling required for integrin αvβ5-directed cell motility but not adhesion on vitronectin. J Cell Biol 1994; 127: 859-66.
- 28 Andrews RK, Kroll MH, Ward CM, Rose JW, Scarborough RM, Smith AI, Lopez JA, Berndt MC. Binding of a novel 50-kilodalton alboaggregin from Trimeresurus albolabris and related viper venom proteins to the platelet membrane glycoprotein Ib-IX-V complex. Biochemistry 1996; 35: 12629-39.
- 29 Clemetson JM, Polgar J, Magnenat E, Wells TNC, Clemetson KJ. The platelet collagen receptor glycoprotein VI is a member of the immunoglobulin superfamily closely related to FcαR and the natural killer receptors. J Biol Chem 1999; 274: 29019-24.
- 30 Dormann D, Clemetson JM, Navdaev A, Kehrel BE, Clemetson KJ. Alboaggregin A activates platelets by a mechanism involving glycoprotein VI as well as glycoprotein Ib. Blood 2001; 97: 929-36.
- 31 Miura Y, Ohnuma M, Jung SM, Moroi M. Cloning and expression of the platelet-specific collagen receptor glycoprotein VI. Thromb Res 2000; 98: 301-9.
- 32 Gu M, Xi X, Englund GD, Berndt MC, Du X. Analysis of the roles of 14-3-3 in the platelet glycoprotein Ib-IX-mediated activation of integrin αIIbβ3 using a reconstituted mammalian cell expression model. J Cell Biol 1999; 147: 1085-96.
- 33 Yeh C-H, Wang W-C, Hsieh T-T, Huang T-F. Agkistin, a snake venom-derived glycoprotein Ib antagonist, disrupts von Willebrand factor-endothelial cell interaction and inhibits angiogenesis. J Biol Chem 2000; 275: 18615-8.