Thromb Haemost 2001; 85(03): 464-469
DOI: 10.1055/s-0037-1615606
Review Article
Schattauer GmbH

Depletion of Plasma Factor XIII Prevents Disseminated Intravascular Coagulation-induced Organ Damage

Soo Young Lee
1   The Catholic University of Korea, Department of Natural Sciences, College of Medicine, Seoul, Korea, Mogam Biotechnology Research Institute, Yongin, Korea
,
Sung Keun Chang
2   Soonchunhyang University, Department of Chemistry, Asan, Korea
,
Il Ha Lee
1   The Catholic University of Korea, Department of Natural Sciences, College of Medicine, Seoul, Korea, Mogam Biotechnology Research Institute, Yongin, Korea
,
Yu-Mie Kim
1   The Catholic University of Korea, Department of Natural Sciences, College of Medicine, Seoul, Korea, Mogam Biotechnology Research Institute, Yongin, Korea
,
Soo Il Chung
1   The Catholic University of Korea, Department of Natural Sciences, College of Medicine, Seoul, Korea, Mogam Biotechnology Research Institute, Yongin, Korea
3   Mogam Biotechnology Research Institute, Yongin, Korea
› Author Affiliations
Further Information

Publication History

Received 30 May 2000

Accepted after resubmission 23 October 2000

Publication Date:
08 December 2017 (online)

Summary

The impact of clot stability affecting the vasculopathy and tissue necrosis in Shwartzman reaction was investigated using plasma Factor XIII A2-depleted rabbit (FXIII-DR). Plasma Factor XIIIA2 (FXIIIA2) was depleted by infusion of the mono-specific goat anti-rabbit FXIIIA2 IgG. Generalized Shwartzman reaction (GSR) was induced by priming and challenged by i.v. injection of LPS and local Shwartzman reaction (LSR) was primed by intradermal injection of LPS and challenged by i.v. injection of LPS. Histological examination of the GSR animals showed, extensive thrombi accumulation in renal tubules and bilateral cortical necrosis of kidney in 8 out of 10 rabbits but none in the FXIIIDR. Fibrinogen levels were elevated to 3~4 fold at 24 h and lowered at 48 h whereas a steady rise was seen in the FXIII-DR. FDP levels in GSR animals were significantly elevated at 24 h and further increased at 48 h but only slightly elevated in the FXIII-DR. Examination of the LSR tissues after 48 h showed an acute onset of progressive cutaneous vascular thrombosis, purpura, and secondary hemorrhagic necrosis whereas neither fibrin deposit nor necrosis of tissue were detected in FXIII-DR despite of an early edema formation. Fibrinogen levels were also increased two fold at 24 h but returned to basal levels at 48 h in control LSR animals but not affected at all in FXIII-DR. These results suggest that during the severe inflammatory conditions such as sepsis, the fibrinolytic system is functionally sufficient to dissipate the pathogenic accumulation of disseminated intravascular clots and exudated fibrin clots if those clots were prevented from getting cross-linked in plasma.

 
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