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Thromb Haemost 2000; 83(03): 427-432
DOI: 10.1055/s-0037-1613832
Review Article
Schattauer GmbH

Haemostatic Factors in Human Peripheral Afferent Lymph

George J. Miller
1   From the Medical Research Council Epidemiology and Medical Care Unit, London, UK
,
David J. Howarth
1   From the Medical Research Council Epidemiology and Medical Care Unit, London, UK
,
Janet C. Attfield
1   From the Medical Research Council Epidemiology and Medical Care Unit, London, UK
,
C. Justin Cooke
2   Department of Cardiovascular Biochemistry, St Bartholomew ‘s and the Royal London School of Medicine and Dentistry, London, UK
,
M. Nazeem Nanjee
2   Department of Cardiovascular Biochemistry, St Bartholomew ‘s and the Royal London School of Medicine and Dentistry, London, UK
,
Waldemar L. Olszewski
3   Department of Surgical Research and Transplantology, Medical Research Council, Polish Academy of Sciences, Warsaw , Poland
,
James H. Morrissey
4   Oklahom a Medical Research Foundation, Oklahoma City, Oklahoma, USA
,
Norman E. Miller
2   Department of Cardiovascular Biochemistry, St Bartholomew ‘s and the Royal London School of Medicine and Dentistry, London, UK
› Author Affiliations
Further Information

Publication History

Received 21 July 1999

Accepted after rebsubmission 22 November 1999

Publication Date:
14 December 2017 (online)

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Summary

Peripheral afferent lymph was obtained by cannulation of a collecting vessel in 17 healthy men (mean age 26 years). Lymph/plasma ratios of all vitamin K-dependent factors were lower than expected from molecular weight. Factor VII, factor IX and tissue factor pathway inhibitor (TFPI) lymph/plasma activity ratios were higher than antigen ratios. Activated factor VII (FVIIa) and TFPI-Xa complex concentrations were higher in lymph than plasma, and the raised FVIIa did not appear to be due to cannulation. The fibrinogen lymph/plasma activity (Clauss) ratio averaged about 20% of the antigen ratio. The result of an ELISA for D-dimer was higher in lymph than plasma, often more than five-fold. This high level in lymph was not explored but may indicate proteolysis of fibrinogen and fibrin with release of D-like and D-dimerlike fragments in interstitial fluid.

Keywords

Peripheral lymph - haemostatic factors - D-dimer
 

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