Platelet Associated u-PA Up-regulates u-PA Synthesis by Endothelial Cells

L. Camoin-Jau; R. Pannell; F Anfosso; N. Bardin; F. Sabatier; J. Sampol; V. Gurewich; F. Dignat-George

doi:10.1055/s-0037-1613246

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2002; 88(03): 517-523
DOI: 10.1055/s-0037-1613246

Review Article

Schattauer GmbH

Platelet Associated u-PA Up-regulates u-PA Synthesis by Endothelial Cells

L. Camoin-Jau

¹INSERM EMI 00-19, Laboratoire d’Hématologie, Univ Méditerra née, UFR de Pharmacie and Hôpital de la Conception, Marseille, France

,

R. Pannell

²Vascular Research Laboratory, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA

,

F Anfosso

¹INSERM EMI 00-19, Laboratoire d’Hématologie, Univ Méditerra née, UFR de Pharmacie and Hôpital de la Conception, Marseille, France

,

N. Bardin

¹INSERM EMI 00-19, Laboratoire d’Hématologie, Univ Méditerra née, UFR de Pharmacie and Hôpital de la Conception, Marseille, France

,

F. Sabatier

¹INSERM EMI 00-19, Laboratoire d’Hématologie, Univ Méditerra née, UFR de Pharmacie and Hôpital de la Conception, Marseille, France

,

J. Sampol

¹INSERM EMI 00-19, Laboratoire d’Hématologie, Univ Méditerra née, UFR de Pharmacie and Hôpital de la Conception, Marseille, France

,

V. Gurewich

²Vascular Research Laboratory, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA

,

F. Dignat-George

¹INSERM EMI 00-19, Laboratoire d’Hématologie, Univ Méditerra née, UFR de Pharmacie and Hôpital de la Conception, Marseille, France

› Institutsangaben

Abstract

Summary

Adhesion of platelets to endothelium has been shown to induce important changes in endothelial properties. In this study, we examined the effect of platelet-endothelial cell interactions on the expression of urokinase-type plasminogen activator (u-PA) by human microvascular endothelial cells. After incubation of endothelial cells with platelets, a dose-dependent increase in the expression of u-PA Ag was observed and reached a plateau for a ratio of 300 platelets per endothelial cells. The u-PA Ag upregulation resulted from an increase in u-PA mRNA that originated from a synthesis by endothelial cells since no u-PA mRNA was detected in platelets. The platelet-induced u-PA synthesis was inhibited when the endothelial cells were pre-treated with phospholipase C to remove the u-PA receptor, or when the platelets were incubated with an antibody that blocks the binding of u-PA to u-PAR. Taken together, these data indicate that u-PA present on the platelet surface interacts with u-PAR on the endothelial cells and induces the u-PA synthesis. This mechanism may represent a physiological control of platelet-mediated intravascular fibrin deposition.

Keywords

Urokinase - platelets - endothelial cells - urokinase-plasminogen receptor

Volltext

Referenzen

References
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