A 28-kDa Glycoprotein Functions as a Platelet Ligand for P-selectin (CD62P)^[*]

Summary

P-selectin (CD62P) is expressed on activated platelets and on stimulated endothelial cells. It interacts with P-selectin glycoprotein ligand-1 (PSGL-1; CD162) for adhesion of activated platelets on leukocytes and for rolling of leukocytes on stimulated endothelial cells. Recently, resting and activated platelets have been shown to roll on endothelial P-selectin, indicating that platelets express (a) ligand(s) for P-selectin. Here we show that P-selectin specifically precipitated one 28-kDa glycoprotein from the whole cell lysates and the membrane lysates of human platelets in a Ca²⁺-dependent manner. Further, the purified 28-kDa molecule could inhibit the binding of P-selectin to human resting and activated platelets. In contrast, KPL1 (a leukocyte adhesion blocking MoAb to PSGL-1) did not neutralize the binding of P-selectin to human platelets, even though it abolished the binding of P-selectin to human promyeloid HL-60 cells. Our results thus indicate that the 28-kDa glycoprotein may function as an important platelet ligand for P-selectin.

Keywords

Platelets - P-selectin (CD62P) - thrombosis - glycoprotein - ligand

*Supported by grants from Chinese Academy of Sciences (KSCX2-2-02), National Natural Science Foundation of China (39925015, 30130090), Special Funds forMajor State Basic Research of China (Grant G1999053907)

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