Dedicated to the memory of the late Professor Sho Ito
Published as part of the 50 Years SYNTHESIS – Golden Anniversary Issue
Key words
isoflavonoid - rotenoid - semi-pinacol rearrangement - S
NAr cyclization - total synthesis
1
Introduction
We wish to report a general synthetic route to rotenoids, a class of plant-derived
natural products of traditional importance as well as of recent interest by newly-found
biological activities. Our full account is structured as follows, (1) introduction,
including historic interest, biosynthesis, and previous syntheses, and (2) syntheses
of several rotenoids via our present strategy.
1.1
Historic Interest in Rotenoids
In tropical regions in East Asia and South America, various leguminous plant species,
including Derris and Lonchocarpus, have traditionally been used as insecticides and fish poison. The latter is associated
with ‘lazy fishing’, that is, dusting the powdered root on the water surface and collecting
the floating fish, which can be eaten.
Research on the toxic ingredients led to the isolation of a series of compounds, termed
as the rotenoids (Figure [1]).[1] The major component, rotenone (1), was isolated as early as 1896,[2] and its structure was elucidated in 1932 by three independent groups led by Takei,[3a] Butenandt,[3b] and LaForge.[3c] The absolute stereochemistry of 1 was determined by Büchi in 1961.[4] Other minor congeners, deguelin (2) and tephrosin (3), were isolated in 1931 by Clark.[5a]
[b] In 1932, the structures of 2 and 3 were assigned by Clark[5c] and by Butenandt,[5d] respectively. It is interesting to note that the name, rotenone, originated from the Taiwanese name of the plant (Figure [2]) in combination with the ketone functionality, that is, ‘roten’ (Fish wisteria)
+ ‘one’.
Figure 1 Natural rotenoids
Their toxicity originates from the interference of the ubiquinone oxidoreductase of
the respiratory electron transport chain.[1] Upon ingestion, the compounds are relatively innocuous to mammals, being rapidly
metabolized, while fish and insects lack such a detoxification mechanism. Frightening
enough, however, a recent report stated that 1 and 2 are causative agents of Parkinson disease.[6] On the other hand, significant reports have appeared on the antitumor effects of
2
[7a] and 3,[7b] which evoked considerable attention of biological research and also chemical synthesis.
Figure 2 Prof. C.-C. Liao, Taiwan, shows roten plant, recalling his childhood
1.2
Biosynthesis
As a subclass of the isoflavonoid natural products, the biosynthesis of rotenoids
starts with the assembly of the polyketide–shikimate scaffold A (Scheme [1]).[1]
[8] Claisen condensation and oxy-Michael reaction give flavanone B, which undergoes P-450-initiated generation of a radical species, inducing 1,2-shift of an aryl group to form isoflavanone C, and dehydration to give isoflavone D. After installation of additional oxygen functions on the A ring and methylations
to give E, oxidative transformation of the O-methyl group triggers a cyclization to form the B-ring as in H. Installation of an isoprenyl group forms rotenoic acid (I), which is a branching point to rotenone (1) via a 5-exo-cylization and deguelin (2) via a 6-endo-cyclization.
Scheme 1 Rotenoid biosynthesis
1.3
Synthetic Studies
Synthetic studies of rotenoids started in the mid-20th century, and early successes
include the total syntheses of (±)-1 (Matsui,[9] 1960), (±)-2 [(Fukami,[10a]
[b] 1960) and (Yamashita,[10c] 1974)], and (–)-1 (Yamashita,[11] 1979). After a hiatus, synthetic interest has recently resurged by the discovery
of novel bioactivities in minor rotenoids, including 2 and 3. Since rotenone (1) is readily available from natural sources, several semi-syntheses of 2 from 1 have been devised.[12] However, total synthesis reports have appeared as well, including (±)-2 [(Sames,[10d] 2003) and (Xu,[10e] 2018)], and (–)-2 [(Winssinger,[13a] 2010), (Scheidt,[13b] 2013), and (Suh,[13c] 2015)]. Approaches to tephrosin (–)-3 (Winssinger,[13a] 2010) and of (±)-3 (Xu,[10e] 2018) have appeared as well.
In connection with our synthetic studies on the flavonoid- and isoflavonoid-class
of polyphenols, we became interested in the synthesis of the rotenoids. In due course,
we reported the total syntheses of (–)-1 and (–)-dalpanol (4) in 2016 as a rapid communication.[14] The purpose of this paper is to outline our general synthetic approach, featuring
the use of the group-selective, stereospecific 1,2-rearrangements of epoxy alcohol
J followed by folding the product K into the tetracyclic scaffold L by dual SNAr oxy-cyclizations of an aryl fluoride by an internal alkoxide (Scheme [2]).
Scheme 2 Our synthetic approach to rotenoids
Before going into the detail, it would be appropriate to give a small overview of
the semi-pinacol-type 1,2-shifts, centering attention to the group selectivity and
the stereochemical integrity.
1.4
General Issues of 1,2-Rearrangements
Concerning the semi-pinacol rearrangement of compounds with the general formula M, let us focus on the following two aspects (Figure [3]).
Figure 3 Two aspects of semi-pinacol rearrangement
The group selectivity refers to the selectivity, among the two potential migrating groups, A and B, which
undergoes the 1,2-shift. Two factors are relevant, namely a) migratory aptitudes of
A and B, and b) effect of the stereochemistry of the reactant M.
In addition, the reaction may proceed either with inversion of the pre-existing stereogenic
center (stereospecific) or with racemization, depending on the nature of the reaction, reflecting the concerted
or stepwise nature of the bond reorganization events, namely departure of the leaving
group, and the 1,2-shift.
1.4.1
Curtin–Collins Experiments
Around 1950, Curtin published the pioneering work[15] on the effect of the stereochemistry (conformation/configuration) of reactants on
the reactivity (Scheme [3]). Deaminative semi-pinacol rearrangement was the subject that led him to a concept,
later called as the Curtin–Hammett principle.[16] Diastereomeric amino alcohols Ia and Ib, upon diazotization, gave markedly different product distributions. While Ia mostly gave II by the anisyl shift, and a minor amount of III was formed by the the phenyl shift. The tendency was opposite for the diastereomer
Ib, giving the phenyl-shifted product III as the major product. The latter example is striking in view of the high migratory
aptitude of an anisyl group, 103 times higher than that of a phenyl group in pinacol rearrangement,[17] which clearly shows the importance of the stereochemistry of the reactants.
Scheme 3 Curtin’s experiments
In 1957, Collins provided insight into the conformational factor using ingeneously
designed tracer experiments (Scheme [4]).[18] The semi-pinacol rearrangement of chiral, non-racemic (S)-IV gave mostly V (inversion), but with partial racemization (76% ee). The same experiment, but using
stereospecifically labeled IV′ (Ph* designates a 14C-labeled phenyl group), showed that the inversion product, (S)-V′, is produced by the Ph* shift, while the retention product, (R)-V′, is derived from the Ph shift. The implication is that the reaction proceeds via
an open carbenium ion, which does not last long enough for the free bond roation around
the C–C bond. Reflecting the most stable conformer of the parent diazonium ion VI, the initially-formed carbenium ion is VIIa, which undergoes the 1,2-shift of Ph* (inversion), while a competing C–C bond rotation of VIIa allows a partial leakage to the second carbenium ion conformer VIIb, which undergoes 1,2-shift of the Ph group (formally retention).
Scheme 4 Collins’ experiments
Overall, due to the super-leaving ability of N2 from aliphatic diazonium salts, the reaction takes on a typical SN1 character, losing the stereochemical integrity. It was thus pointed out that the
stereospecific 1,2-shift would become possible, if suitable conditions were set to
achieve an internal SN2 process, which was indeed achieved as follows.
1.4.2
Pinacol-Type Rearrangements of α-Mesyloxy Alcohols Promoted by Organoaluminum Reagents
In 1983, we reported that chiral, non-racemic methanesulfonyloxy alcohol VIII, upon treatment with Et3Al as a Lewis acid, undergoes stereospecific 1,2-rearrangement with inversion of the
pre-existing stereogenic center (Equation 1).[19] Importantly, even when the the starting material is a diastereomeric mixture at
the tert-alcohol center, the 1,2-shift takes place in a group-selective manner, if the potential
migrating groups differ significantly in their migratory aptitudes. The Lewis acid
activation through a seven-membered chelate A allows a smooth reaction to proceed. Flexibility of the seven-membered chelate explains
the selective migration of the group of higher migratory aptitude by placing itself
at the antiperiplanar position to the leaving group.
Equation 1 Et3Al-promoted pinacol-type rearrangement
On the other hand, Equation 2 exemplifies a reaction where the tert-alcohol stereogenic center is decisive for the group selectivity.[20] Note that the potential migrating groups, ethyl and octyl, are of essentially the
same migratory aptitudes. Under carefully defined conditions to generate an aluminum
alkoxide, a high group selectivity is observed as accounted by the chelation model.
The 1,2-shift of alkyl groups is also stereospecific.
Equation 2 Competition of two alkyl groups
1.4.3
Epoxy Alcohol →Aldol Rearrangements
In 1986, we published a joint paper[21a] with the Yamamoto–Maruoka group at Nagoya, reporting Lewis acid promoted rearrangements
of epoxy alcohols and the corresponding silyl ethers into aldol products.[21a] Later, we exploited the synthetic utility of the 1,2-shift-based aldol synthesis
in various natural product syntheses.[21b]
[c]
[22] Scheme [5] illustrates a divergent syntheses of antifungal natural products, avenaciolide and
isoavenaciolide, featuring several important aspects of synthetic utilities.[21b]
[c] First, the 1,2-shift proceeds stereospecifically, allowing clean conversions of
trans-epoxy alcohols, XII and XV, into anti-aldols, XIII and XVI. Although not shown, vice versa is true in converting cis-epoxy alcohols into syn-aldol compounds. Second, although the starting materials XII and XV are diastereomeric mixtures, exclusive migration of the vinyl groups occur, which
could be ascribed to the relative migratory aptitudes (vinyl >> alkyl). Conformational
flexibility allows both diastereomers to adopt the respective ‘reactive conformers’
placing the vinyl group antiperiplanar to the epoxide C–O bond to be cleaved upon
Lewis acid activation, manifesting a typical Curtin–Hammett system.[16] Third, note that an α-silylvinyl group has an excellent migratory aptitude, which
was previously discovered in the pinacol-type rearrangement.[23] Also interestingly, depending on the presence or the absence of TMS group, the stereochemical
course of the reduction of aldol products is different, as explained by the hydrogen-bonded
models A and B, respectively.[21c] These features were exploited in the present project as will be discussed later.
Scheme 5 Epoxy alcohol → aldol rearrangements and stereoselsective reduction: divergent syntheses
of avenaciolide and isoavenaciolide
2
Results and Discussion
2.1
Synthetic Planning – A Thought Process
In the following, the thought process how our synthetic plan evolved will be described.
The starting point was our recent study on the flavonoid- and isoflavonoid-class natural
products, through which two powerful tactics relevant to the rotenoid synthesis have
been developed.
Tactic #1 was the SNAr oxy-cyclization of aryl fluorides,[24] working even without resorting to electron-withdrawing group(s), such as a nitro
group (Equation 3).[24b]
Equation 3 Tactic #1: SNAr oxy-cyclization of aryl fluorides
Tactic #2 was an approach to isoflavonoids based on the 1,2-shift of flavonoids inspired
by the biosynthesis (Equation 4).[25] Activation of catechin-derived mesylate XX with an organoaluminum reagent effects 1,2-shift of an aryl group, and the intermediary
oxonium species is captured by an aluminum ligand, giving XXI. The process is characterized by a thorough stereospecificity (perfect enantiomeric
excess) and a perfect trans-selectivity.
Equation 4 Tactic #2: 1,2-Shift approach to isoflavonoids
For the rotenoid synthesis, however, application of tactic #2 was unrealistic for
two reasons, (1) the 2,3-cis stereochemistry was required, and (2) finding a ‘–CH2OH’ equivalent was not straightforward (Scheme [6]A).
Scheme 6 Thought process
Aa an alternative, we came up with an idea of a similar 1,2-shift, but placing the
migrating aryl group at the C-4 position rather than at the C-2 position (Scheme [6]B). Still there was a problem, in that stereoselective preparation of the starting
material C seemed uneasy.
As a potential countermeasure, we centered our attention to the epoxy alcohol → aldol
rearrangement.[21] If one started with cis-epoxy alcohol E, stereospecific 1,2-shift of an aryl group would give syn-aldol F (Scheme [6]C). We selected two aryl groups possessing an o-fluoro group, expecting their utilities for the construction of the B and C rings
by means of SNAr reactions. A key question was the group selectivity in the 1,2-rearrangement. While the competing shift of the D-ring (red) gives the
isomeric product (not shown), the desired product F is obtained by the 1,2-shift of the A-ring (blue). Given the latter case, the aldol
F has a functional pattern ideally suited for constructing two tetrahydropyran rings
by dual SNAr oxy-cyclizations.
As discussed above, such group selectivity could be influenced by the relative migratory
aptitude and/or the conformational effect. In the present case, both aryl groups share
a similar substitution pattern possessing an o-fluoro group, and thus, their intrinsic migratory aptitudes appeared to be similar.
Therefore, our initial study was centered at examining the group selectivity of the
1,2-rearrangement by using a substrate within the context of the rotenone synthesis.[14]
2.2
Preliminary Study on 1,2-Rearrangement
Scheme [7] shows the preparation of the substrate 9 for the 1,2-shift, in which we arbitrarily installed the DE-ring first followed by
the A-ring.[14] Fluorobenzene 6 was lithiated (s-BuLi, Et2O, TMEDA, –78 °C, 1 h) and combined with chiral, non-racemic epoxy amide 5, giving epoxyketone 7 in 72% yield. Bromide 8
[26] was subjected to bromine–lithium exchange (n-BuLi, Et2O, –78 °C, 1 h) and combined with ketone 7, where stereoselective reaction occurred to give epoxy alcohol 9 as a single product. The stereochemical course of the addition could be explained
by chelation model A. Notably, an excellent stereoselectivity was observed, which could be due to the
presence of the cis-substituent that effectively blocks the nucleophilic attack from the right side.[27]
Scheme 7 Preliminary experiment 1 (substrate preparation)
Scheme 8 Preliminary experiment 2 (1,2-shift/reduction)
Scheme [8] shows the key 1,2-rearrangement of epoxy alcohol 9.[14] Upon treatment with BF3·OEt2 (CH2Cl2, 0 °C), epoxy alcohol 9 smoothly reacted within 20 minutes. Assuming the potential lability of the aldol
products (e.g., undergoing dehydration, retro-aldol reaction and/or epimerization), the crude products were treated with NaBH4 in methanol. Diol 11 was obtained as the single product, derived from the 1,2-shift of the DE-ring unit
(red). Unfortunately, the wrong group underwent migration regarding the anticipated
total synthesis of 1. Importantly, however, we were able to understand the stereochemical course of the
reactions by careful 1H NMR analysis, after conversion of diol 11 into anisylidene acetal 12. Two conclusions were: (1) the 1,2-shift occurred stereospecifically with an inversion,
and (2) the reduction of the aldol product 10 was stereoselective, as rationalized by model B.[21c]
Even though the undesired isomer was obtained, the perfect group selectivity gave us valuable insight. Scheme [9] shows two hydrogen-bonded conformers of epoxy alcohol 9, where conformer 9b is disfavored by steric hindrance caused by the cis-substituent R (CH2OTBS). Conformer 9a would be highly populated, a hypothesis, which was supported by calculations on a
simple model substrate (R = Me, and aryl = Ph), showing an energy difference as large
as 5.4 kcal/mol.
Scheme 9 Rationale for the group-selectivity
Assuming 9a to be essentially the sole conformer present, the D-ring (red) undergoes 1,2-migration,
since it is antiperiplanar to the C–O bond (green) that is cleaved upon Lewis acid
activation. Note that this interpretation does not contradict the Curtin–Hammett principle,[16] and just corresponds to one of the prototypical categories, where both conformers
react at a similar rate (i.e., similar migratory aptitudes), and the conformer ratio
(virtually exclusively 9a) is reflected in the product distribution.
This result gave us a clear and simple guideline to achieve the group-selective 1,2-rearrangement
(Scheme [10]): An ‘empirical rule’ is ‘Install the migrating group first!’. The hope was simply
that, by reversing the order of installing two aryl groups (i.e., first the A-ring
to give II followed by the D-ring), the diastereomeric substrate III would be produced, which in turn would undergo 1,2-shift of the A-ring in a group-selective
manner.
Scheme 10 Guideline for the group-selective 1,2-shift
To our delight, this scenario has been successfully realized, allowing a unified synthetic
route to the total syntheses of (–)-rotenone (1) and (–)-deguelin (2). Although the synthesis of 1 has been reported as a communication,[14] sizable improvements have been made thereafter, which will be described in the following.
2.3
Synthesis of (–)-Rotenone (1)
Total synthesis of (–)-rotenone (1) was executed as follows: In comparison with our previous report,[14] one of the improvements is the use of epoxy lactone 16 as a chiral, non-racemic starting material, easily prepared in three steps from d-araboascorbic acid (13), an abundant feedstock (Scheme [11]). Oxidation of 13 with H2O2 following an Organic Synthesis procedure[28] (Na2CO3, H2O, 40 °C, 30 min) with a modified workup gave diol 14 in 94% yield. Regioselective tosylation of 14 (TsCl, pyridine, 0 °C, 14 h) gave tosylate 15 in 76% yield.[29] Treatment of tosylate 15 with K2CO3 (MeCN, rt, 22 h) gave epoxy lactone 16 in 75% yield via the epimerization at C-2 followed by oxirane formation.[30]
Scheme 11 Improved preparation of key intermediate 17
Bromobenzene 8 was treated with n-BuLi (Et2O, –78 °C, 1 h) to effect a halogen–metal exchange, and the resulting lithio species
was combined with lactone 16 to give adduct 17, which was in an equilibrium with hemiacetal 18. The 17/18 mixture was treated with tert-butyldimethylsilyl chloride (TBSCl) and imidazole, giving siloxy ketone 19 in 94% yield (2 steps).
Ketone 19 is a common synthetic intermediate of our previous synthesis of 1
[14] as well as the synthesis of deguelin (2) as will be described later. The availability of ketone 19 was significantly improved in a total yield of 50% over five steps, starting from
13. The previous approach used amide 5 as the chiral, non-racemic building block, which was only available in 15% yield
in nine steps from diethyl l-tartrate.[14]
Next, the DE-ring unit 6 was lithiated (s-BuLi, Et2O, TMEDA, –78 °C, 1 h), and allowed to react with ketone 19 to give epoxy alcohol 20 in 89% yield (Scheme [12]). As expected, epoxy alcohol 20 was obtained as a single diastereomer, which proved to be epimeric to 9.[14] Pleasingly, the reaction of epoxy alcohol 20 with BF3·OEt2 (20 mol%, CH2Cl2, 0 °C, 15 min), followed by the reaction with NaBH4 cleanly gave diol 21 as a single isomer in 71% yield. It should be noted that 21 was the product that is derived from the migration of the A-ring (blue) (cf. diol
11) as ascertained by extensive NMR study.[14] The stereochemical course of the two-step reaction 20 → 21 (1,2-shift followed by reduction) proved perfect by the careful analysis after conversion
into anisylidene acetal 22, which also served as an advance intermediate en route to (–)-rotenone (1).
Scheme 12 Key 1,2-rearrangement
Acetal 22 was converted into the pentacyclic rotenoid skeleton via two SNAr oxy-cyclizations (Scheme [13]). Upon treatment of 22 with n-Bu4NF (THF, rt, 1 h), the TBS group was removed, giving alcohol 23 in 98% yield, ready for the SNAr oxy-cyclization. After screening of the conditions, the projected reaction was
achieved by using t-BuOK in the presence of catalytic amounts of Ni(cod)2 (10 mol%) and PCy3 (30 mol%) (toluene, reflux, 2 h), giving tetrahydropyran 24 in 86% yield. Upon treatment with AlH3,[31] anisylidene acetal 24 was regioselectively cleaved, giving alcohol 25 as a single isomer (79% yield). The SNAr oxy-cyclization of 25 proceeded smoothly (NaH, 15-crown-5, toluene, DMPU, 80 °C), giving pentacycle 26 in 91% yield. The MPM protecting group in 26 was detached by hydrogenation [H2, Pd(OH)2/C, THF, t-BuOH, H2O, rt, 3 h] to give alcohol 27 in 80% yield, which was oxidized with 2-iodobenzoic acid (DMSO, rt, 5 h) to give
ketone 28 in 85% yield. Removal of the MOM group in 28 (aq HCl, MeOH, 50 °C, 1.5 h) gave (–)-dalpanol (4) in 89% yield. Recrystallization from benzene gave 4 as colorless needles {mp 199–200 °C, [α]D
22 –1.1 × 102 (c 0.52, CHCl3) [Lit.[32a] mp 196 °C, [α]D
22 –136.3 (c 0.62, CHCl3)]}. All the physical data of the synthetic sample of 4 (1H and 13C NMR, IR, HRMS) coincided with the reported data.[32]
Scheme 13 Endgame to rotenone (1)
Finally, treatment of 4 with Burgess reagent[33] A gave 1 in 50% yield. A side product, benzofuran 29, was obtained in 13% yield, arising most likely from the tertiary cation generation
followed by a 1,2-hydride shift. Recrystallization from benzene gave 1 as colorless crystals {mp 153–154 °C, [α]D
23 –1.5 × 102 (c 0.070, CHCl3) [Lit.[11] mp 165–166 °C, [α]D
23 –177 (c 2, CHCl3)]}. All the physical data of the synthetic sample of 1 coincided with the reported data.[11]
[34] Direct comparison was done with an authentic sample (1H and 13C NMR, IR, HRMS).[35]
2.4
Total Synthesis of (–)-Deguelin (2)
Since deguelin (2) is one of the rotenoids that is attracting recent interest by its anticancer activity,[7a] we decided to apply the above-stated synthetic route to the synthesis of 2, as described in this section.
Scheme [14] shows the preparation of the DE-ring unit 34 for the synthesis. 3-Fluorophenol (30) was protected as a THP ether to give fluorobenzene 31 (88% yield).[36] Regioselective lithiation of 31 (n-BuLi, HMPA, THF, –78 °C, 1 h) and treatment with prenyl bromide (THF, –78 °C, 1 h)
gave the prenylated product 32, which was hydrolyzed (cat. PPTS, EtOH, 60 °C, 6 h) to give phenol 33 in 92% yield (2 steps). Oxidative cyclization of phenol 33 using PdCl2 and CuCl2 under air[37] gave the DE-ring unit 34 in 74% yield.
Scheme 14 DE-Ring building block for the synthesis of deguelin
Scheme [15] illustrates the synthesis of epoxy alcohol 35 for the projected 1,2-shift. ortho-Lithiation of 34 with s-BuLi (Et2O, TMEDA, –78 °C, 1 h) followed by reaction with ketone 19 gave epoxy alcohol 35 in 85% yield as a single diastereomer. The stereostructure of 35 was assigned as shown based on 1H NMR and NOE analyses. The projected 1,2-rearrangement of epoxy alcohol 35 was achieved by treatment with BF3·OEt2 (20 mol%, CH2Cl2, –15 °C, 40 min). The crude material containing aldol 36 was immediately reduced with i-Bu2AlH, giving diol 37 as a single diastereomer in 88% yield (2 steps). HMBC-analysis shown below verified
that diol 37 was derived from the 1,2-shift of the A-ring. The stereochemical relations of C-2,
C-3, and C-4 stereogenic centers were concluded at the stage of anisylidene acetal
38, which was obtained by acetalization of 1,3-diol 37 followed by removal of the TBS group in 89% yield (2 steps). The stereochemistry
was identified as such by NOE analyses, verifying that (1) the stereospecificity of
the 1,2-shift (inversion), and (2) the facial selectivity of the i-Bu2AlH reduction.
Scheme 15 Synthesis of diol 37 via 1,2-shift and reduction
Having acetal 38 as an advanced intermediate, the next stages were the formations of the B- and C-pyran
rings by dual SNAr oxy-cyclizations (Scheme [16]). The SNAr reaction of 38 proceeded smoothly by the action of t-BuOK (3.0 equiv, toluene, reflux, 1.5 h), giving ether 39 in 74% yield. It is notable that use of the Ni catalyst was necessary in the corresponding
rotenone synthesis (see 23 → 24, Scheme [13]). By contrast, the permit case, 38 → 39, did not need the Ni catalyst. Treatment of ether 39 with i-Bu2AlH allowed regioselective C–O bond cleavage to give alcohol 40 in 93% yield. The regioselectivity can be explained by Al-coordination to the C-2
oxygen with less steric hindrance.[38] Note that AlH3 was used for this purpose in the synthesis of 1 (see 24 → 25, Scheme [13]). i-Bu2AlH turned out to be superior for this transformation. The second SNAr oxy-cyclization of alcohol 40 proceeded smoothly using NaH [2.0 equiv, 15-crown-5 (1.0 equiv), toluene, DMPU (9:1),
80 °C, 2 h], giving ether 41 in 95% yield.
Scheme 16 Endgame in the synthesis of 2
Finally, ether 41 was converted into the natural product, (–)-deguelin (2). Removal of the MPM group in 41 with DDQ[39] [2,6-di-tert-butylpyridine, 1,4-dioxane, H2O (8:1), 50 °C, 1 h] gave alcohol 42 in 72% yield. We noted that small amounts of diol 43 was formed (11% yield) due to the oxidation at the benzylic position, which was convertible
into (–)-tephrosin (3) – an oxidized rotenoid congener (vide infra). Oxidation of alcohol 42 with IBX (DMSO, rt, 6.5 h) gave (–)-deguelin (2) as a yellow amorphous solid in 82% yield. All the physical data (1H, 13C NMR, IR, high-resolution MS) of the synthetic material 2 coincided with those of the reported data:[40] [α]D
20 –46 (c 0.20, CHCl3) {Lit.[40c] [α]D
20 –45 (c 0.2, CHCl3)}.
2.5
Total Synthesis of (–)-Tephrosin (3)
As noted in the introduction, novel biological activities[7b] in rotenoids have evoked considerable attention to this class of compounds, including
(–)-tephrosin (3).
As described earlier (Scheme [16]), we noted that diol 43, a side product of the oxidative deprotection of 41, could be regarded as an immediate precursor of 3. Indeed, oxidation of 43 (IBX, DMSO, rt, 5.5 h) gave 3 as a white amorphous solid in 82% yield (Scheme [17]). All the physical data (1H, 13C NMR, IR, high-resolution MS) of the synthetic material 3 matched with the reported data:[40b]
[c] [α]D
20 –98 (c 0.20, CHCl3) {Lit.[40c] [α]D
20 –86 (c 0.2, CHCl3)}.
Scheme 17 Conversion of 43 into (–)-3
Furthermore, seeking for a more practical route to 3, we examined the oxidation of the synthetic (–)-deguelin (2). Recently, two reports appeared on this conversion: Russell[41] used K2Cr2O7 for converting (–)-2, obtained from natural rotenone (–)-1, while Xu[10e] reported a protocol, which was applied to the racemate of 2. We tested these protocols and other potential oxidants on our synthetic material
(–)-2, finding interesting difference in the stereochemistry and the product composition,
as described below.
First, the Russell method was applied to (–)-2 [K2Cr2O7, AcOH, H2O (3:1), 60 °C, 0.5 h), which cleanly gave (–)-3 in 94% yield {[α]D
20 –84 (c 0.23, CHCl3)} (Table [1], entry 1). The HPLC analysis using chiral stationary phase proved the enantiomeric
purity of the product within the limit of the analysis [(a); Figure [4]].
Figure 4 Assessment of ee for 3 and 44. Conditions for 3: CHIRALPAK® IB (ϕ 4.6 mm × 250 mm), hexane/EtOAc (4:1); for 44: CHIRALPAK® IF (ϕ 4.6 mm × 250 mm), hexane/EtOAc (3:2); flow rate: 1.0 mL/min, 25 °C, 254 nm.
Table 1 Conversion of (–)-Deguelin (2) into (–)-Tephrosin (3)
 
|
|
Entry
|
Conditions
|
Results
|
|
1
|
K2Cr2O7, AcOH, 60 °C
|
3: 94% (>99% ee)
|
|
2
|
O2, Cu2O, TBD, DMSO, rt
|
3: 53% (~0% ee), 44: 27% (~0% ee)
|
|
3
|
IBX, DMSO, 60 → 80 °C
|
3: 44% (>99% ee), 44: 44% (>99% ee)
|
In contrast, the result was markedly different with the Xu protocol (Table [1], entry 2). Upon exposure of (–)-2 to O2 (1 atm) in the presence of Cu2O and TBD (DMSO, rt, 4.5 h), a separable mixture of two products formed. After separation
by preparative TLC (hexane/EtOAc 1:1), the less polar product (Rf
= 0.65) was the desired product 3 (53% yield), while the more polar one (Rf
= 0.51) was the epimer 44 (27% yield),[42] which is also a natural product, 12a-epi-tephrosin, derived from the same plant that produces 3. To our surprise, the [α]D values of these compounds were almost zero,[43] suggesting almost complete racemization, which proved indeed the case as verified
by the HPLC analyses on chiral stationary phase [(b) and (c) in Figure [4]]. Equation 5 shows a rationale of the racemization at the C-6a center by base-induced
retro-Michael/Michael reaction, proceeding more rapidly than the rate of the C12a hydroxylation.
Equation 5 Racemization at the C-12a center
In addition, IBX worked as an oxidant (DMSO, 60 → 80 °C, 19 h) giving 3 (44%) and 44 (44%) (Table [1], entry 3), respectively. In contrast to the result of the air oxidation stated above,
both products 3 and 44 were respectively enantiopure, [(d) and (e) in Figure [4]]. This result could be explained by an intramolecular oxygen transfer (Equation
6), albeit with no diastereofacial selectivity.
Equation 6 Intramolecular oxygen transfer
3
Conclusions
In conclusion, a general synthetic route for the rotenoid class of natural products
has been developed by exploiting 1,2-rearrangement and SNAr oxy-cyclizations. The present method realized a facile construction of the benzopyran
structure. The viability has been demonstrated by the synthesis of (–)-rotenone and
(–)-deguelin and also its conversion into (–)-tephrosin and (+)-12a-epi-tephrosin. The present approach provides a means of comprehensive synthesis of rotenoid-related
compounds of biological interest.
All reactions dealing with air- and/or moisture-sensitive reagents were performed
in dried glassware under an atmosphere of dry argon. Ethereal solvents, CH2Cl2 and toluene were used as received (anhydrous; Kanto Chemical Co., Inc.). DMF, HMPA,
TMEDA, and DMPU were distilled prior to use according to standard protocols. For TLC
analysis, Merck pre-coated plates (TLC silica gel 60 F254, Art 5715, 0.25 mm) was used. Silica gel preparative TLC (PTLC) was performed using
plates prepared from Merck silica gel 60 PF254 (Art 7747). For flash column chromatography, silica gel 60N (Spherical, neutral,
63–210 μm) from Kanto Chemical was used. Melting point determinations were performed
using a Yanaco MP-500 instrument or Mettler Toledo MP70 melting point system, and
are uncorrected. 1H, 13C, and 19F NMR were measured on a Bruker Avance III (600 MHz) spectrometer. Chemical shifts
(δ) are expressed in parts per million (ppm) downfield from internal standard (TMS:
δ = 0.00 and hexafluorobenzene: δ = –164.9), and coupling constants are reported in
hertz (Hz). Standard abbreviations were used for splitting patterns. IR spectra were
recorded on Thermo Scientific Nicolet iS5 FTIR spectrometer. Attenuated total reflectance
Fourier transform infrared (ATR-FTIR) spectra were recorded by using Thermo Scientific
Nicolet iS5 FTIR spectrophotometer equipped iD5 ATR accessory. Optical rotations ([α]D) were measured on a Jasco P-3000 polarimeter. High-resolution mass spectra (HRMS)
were obtained with Bruker Daltonics micrOTOF-Q II.
Syntheses and characterization data of compounds 1, 4–7, 9, 11, 12, 20–26, and 29 were reported in our previous paper.[14]
bromobenzene 8
To a solution of 4-fluoro-1,2-dimethoxybenzene (1.00 mL, 7.62 mmol) in CH2Cl2 (15 mL) was added Br2 (0.45 mL, 8.7 mmol) at rt. After stirring for 5 h, the reaction was stopped by adding
sat. aq NaHCO3 and aq 10% Na2S2O3. The crude products were extracted with CH2Cl2 (3 ×), and the combined organic extracts were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by bulb-to-bulb distillation
(140 °C (oven temp)/7.4 mmHg) to afford bromobenzene 8 (1.84 g, quant) as a colorless oil; Rf
= 0.72 (hexane/EtOAc 2:1).
IR (ATR): 3004, 2937, 2360, 1601, 1506, 1439, 1389, 1263, 1215, 1162 cm–1.
1H NMR (600 MHz, CDCl3): δ = 3.848 (s, 3 H), 3.853 (s, 3 H), 6.70 (d, J
C,F = 9.8 Hz, 1 H), 6.96 (d, J
C,F = 6.5 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 56.3, 56.6, 97.5 (d, J
C,F = 22.5 Hz), 100.8 (d, J
C,F = 27.7 Hz), 115.1, 145.9, (d, J
C,F = 2.8 Hz), 149.2 (d, J
C,F = 8.3 Hz), 153.5 (d, J
C,F = 239.4 Hz).
19F NMR (565 MHz, CDCl3): δ = –118.6.
Anal. Calcd for C8H8BrFO4: C, 40.88; H, 3.43. Found: C, 40.73; H, 3.41.
diol 14
To a solution of d-araboascrobic acid (35.2 g, 0.200 mol) in deionized H2O (500 mL) was added Na2CO3 (42.4 g, 0,400 mol) and H2O2 (34.5%, 40 mL, 0.41 mol) at 0 °C over 10 min. After stirring for 30 min at 40 °C,
activated charcoal (Norit A®, 8.0 g) was added. The mixture was stirred for 30 min at the same temperature, and
for 5 min at 80 °C. The reaction mixture was filtered through a Celite pad, washed
with deionized H2O (100 mL), and aq 6 M HCl (150 mL) was added to the filtrate. After concentration
in vacuo, the residue was dissolved in EtOAc, and heated to reflux. The hot mixture
was decanted (5 ×) and the supernatant liquid was cooled to rt. The resulting precipitate
was collected by filtration to afford diol 14 (9.00 g, 38%) as colorless needles. The mother liquor was concentrated in vacuo,
and the residue was purified by recrystallization from EtOAc (3 ×) to afford diol
14 (13.2 g, 56%; total yield: 94%) as colorless needles; Rf
= 0.29 (CHCl3/MeOH 5:1); mp 94–96 °C (EtOAc); [α]D
20 –73 (c 1.00, H2O).
IR (ATR): 3544, 3278, 1744, 1464, 1432, 1229, 1184, 1150, 1027, 953, 771 cm–1.
1H NMR (600 MHz, DMSO-d
6): δ = 4.04 (d, J = 9.9 Hz, 1 H), 4.23 (dd, J = 4.7, 3.0 Hz, 1 H), 4.28 (dd, J = 9.9, 3.0 Hz, 1 H), 4.37 (d, J = 4.7 Hz, 1 H), 5.35 (br s, 1 H, OH), 5.76 (br s, 1 H, OH).
13C NMR (150 MHz, DMSO-d
6): δ = 68.4, 69.5, 71.8, 176.4.
Anal. Calcd for C4H6O4: C, 40.68; H, 5.12. Found: C, 40.81; H, 5.00.
Tosylate 15
To a solution of diol 14 (4.00 g, 33.9 mmol) in pyridine (16 mL) was added TsCl (7.10 g, 37.2 mmol) at 0 °C.
After stirring for 14 h, the pH of the reaction was adjusted to 1 by adding aq 6 M
HCl. The crude product was extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by trituration with CH2Cl2 (3 ×) to afford tosylate 15 (6.95 g, 76%) as a white solid; Rf
= 0.70 (CHCl3/MeOH 5:1); mp 183–184 °C (EtOAc); [α]D
20 –45 (c 1.01, acetone).
IR (ATR): 3470, 1772, 1375, 1192, 1174, 1070, 1024, 816, 776 cm–1.
1H NMR (600 MHz, CDCl3): δ = 2.48 (s, 3 H), 2.78 (br s, 1 H, OH), 4.38 (dd, J = 10.8, 3.6 Hz, 1 H), 4.44 (d, J = 10.8 Hz, 1 H), 4.76 (dd, J = 4.8, 3.6 Hz, 1 H), 5.06 (d, J = 4.8 Hz, 1 H), 7.40 (d, J = 7.8 Hz, 2 H), 7.90 (d, J = 7.8 Hz, 2 H).
13C NMR (150 MHz, CDCl3): δ = 21.8, 68.1, 71.2, 74.3, 128.4, 130.2, 131.6, 146.3, 168.5.
HRMS (ESI): m/z calcd for C11H12O6SNa [M + Na]+: 295.02468; found: 295.0249.
lactone 16
To a solution of tosylate 15 (4.03 g, 14.8 mmol) in MeCN (70 mL) was added K2CO3 (8.06 g, 58.3 mmol) at rt. After stirring for 22 h, the mixture was passed through
a short column of SiO2 and washed with MeCN. The solvent was removed in vacuo, and the residue was purified
by bulb-to-bulb distillation (155 °C (oven temp)/24 mmHg) to afford epoxy lactone
16 (1.11 g, 75%) as a colorless oil; Rf
= 0.50 (CHCl3/MeOH 19:1); [α]D
20 +28 (c 1.04, CHCl3).
IR (neat): 3094, 2971, 1782, 1386, 1365, 1046, 953, 852, 783 cm–1.
1H NMR (600 MHz, CDCl3): δ = 3.81 (d, J = 2.6 Hz, 1 H), 4.22 (dd, J = 2.6, 1.5 Hz, 1 H), 4.32 (dd, J = 11.4, 1.5 Hz, 1 H), 4.47 (d, J = 11.4 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 49.6, 55.0, 68.3, 170.7.
Anal. Calcd for C4H6O4: C, 48.01; H, 4.03. Found: C, 48.20; H, 4.03.
epoxyketone 19
To a solution of bromobenzene 8 (3.38 g, 14.4 mmol) in Et2O (60 mL) was added n-BuLi (1.55 M in hexane, 8.5 mL, 13 mmol) at –78 °C. After stirring for 1 h at –78 °C,
a solution of epoxy lactone 16 (1.21 g, 12.1 mmol) in Et2O (60 mL) was added to the mixture, which was stirred for 1 h at –78 °C. The reaction
was stopped by adding sat. aq NH4Cl. The crude products were extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was dissolved in DMF (60 mL), to which tert-butyldimethylsilyl chloride (2.07 g, 13.7 mmol) and imidazole (1.67 g, 24.5 mmol)
were added. After stirring for 1 h, the reaction was stopped by adding phosphate buffer
(pH 7). The crude products were extracted with Et2O (3 ×), and the combined organic extracts were washed with H2O (2 ×) and brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by trituration with Et2O (4 ×) to afford epoxy ketone 19 (3.72 g, 83%) as a white solid. The mother liquor was concentrated in vacuo, and
the residue was purified by flash column chromatography (SiO2, hexane/EtOAc 4:1) to afford 19 (475 mg, 11%; total yield: 94%) as a white solid; mp 98–99 °C (Et2O); Rf
= 0.56 (hexane/EtOAc 3:1); [α]D
20 +70 (c 1.15, CHCl3).
IR (ATR): 2954, 2930, 2856, 1681, 1611, 1514, 1464, 1450, 1272, 1223, 1142, 1094,
837 cm–1.
1H NMR (600 MHz, CDCl3): δ = –0.09 (s, 3 H), –0.02 (s, 3 H), 0.78 (s, 9 H), 3.56–3.59 (m, 2 H), 3.77–3.80
(m, 1 H), 3.91 (s, 3 H), 3.95 (s, 3 H), 4.26 (dd, J = 5.7 Hz, J
H,F = 4.5 Hz, 1 H), 6.64 (d, J
H,F = 11.9 Hz, 1 H), 7.40 (d, J
H,F = 6.5 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = –5.6, –5.3, 18.3, 25.8, 56.6, 56.7, 58.5 (d, J
C,F = 1.8 Hz, 1 C), 59.5 (d, J
C,F = 12.0 Hz, 1 C), 60.7, 99.9 (d, J
C,F = 30.0 Hz, 1 C), 110.4 (d, J
C,F = 4.8 Hz, 1 C), 115.5 (d, J
C,F = 14.9 Hz, 1 C), 146.0, 155.3 (d, J
C,F = 10.8 Hz, 1 C), 158.9 (d, J
C,F = 251.1 Hz, 1 C), 189.8 (d, J
C,F = 5.1 Hz, 1 C).
19F NMR (565 MHz, CDCl3): δ = –115.1.
HRMS (ESI): m/z calcd for C18H27FO5SiNa [M + Na]+: 393.1504; found: 393.1562.
Alcohol 23
To a solution of acetal 22 (235 mg, 0.322 mmol) in THF (1.8 mL) was added n-Bu4NF (1.0 M in THF, 0.95 mL, 0.96 mmol) at rt. After stirring for 1 h, the reaction
was quenched by adding phosphate buffer (pH 7). The crude products were extracted
with EtOAc (3 ×), and the combined organic extracts were washed with brine, dried
(Na2SO4), and concentrated in vacuo. The residue was purified by flash column chromatography
(hexane/EtOAc 2:1 to 1:1) to afford alcohol 23 (194 mg, 98%) as a white amorphous solid.
Alcohol 27
To a solution of ether 26 (20.1 mg, 0.0346 mmol) in t-BuOH (0.85 mL), THF (0.85 mL), and H2O (0.17 mL) was added ASCA-2 type Pd/C [10% Pd(OH)2/C, 17.5 mg] and stirred under H2 atmosphere at rt for 3 h. After changing the atmosphere from H2 to argon, the reaction mixture was filtered through a Celite pad (washed with CH2Cl2) and concentrated in vacuo. The residue was purified by preparative TLC (hexane/EtOAc
1:1) to afford alcohol 27 (21.9 mg, 80%) as a white amorphous solid.
Ketone 28
To a solution of alcohol 27 (7.6 mg, 0.017 mmol) in DMSO was added IBX (5.3 mg, 0.019 mmol) at rt. After stirring
for 4 h, the reaction mixture was reacted with IBX in two portions (first: 7.8 mg,
0.028 mmol; second: 9.8 mg, 1 h). After stirring for 1 h, the reaction was quenched
by the addition of sat. aq NaHCO3. The crude products were extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by preparative TLC (hexane/EtOAc
1:1) to afford ketone 28 (6.4 mg, 85%) as a white amorphous solid; Rf
= 0.50 (hexane/EtOAc 1:1); [α]D
22 –122 (c 0.980, CHCl3).
IR (neat): 2917, 1673, 1609, 1513, 1456, 1348, 1309, 1197, 1091, 1040 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.29 (s, 3 H), 1.30 (s, 3 H), 3.12 (dd, J = 9.5, 3.7 Hz, 1 H), 3.14 (dd, J = 9.5, 3.7 Hz, 1 H), 3.35 (s, 3 H), 3.80 (s, 3 H), 3.83 (d, J = 3.1 Hz, 1 H), 4.18 (d, J = 12.0 Hz, 1 H), 4.61 (dd, J = 12.0, 3.1 Hz, 1 H), 4.72 (dd, J = 3.7, 3.7 Hz, 1 H), 4.74 (d, J = 7.4 Hz, 1 H), 4.79 (d, J = 7.4 Hz, 1 H), 4.93 (dd, J = 3.1, 3.1 Hz, 1 H), 6.44 (s, 1 H), 6.48 (d, J = 8.6 Hz, 1 H) 6.76 (s, 1 H), 7.81 (d, J = 8.6 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 21.5, 22.7, 27.5, 44.6, 55.3, 55.9, 56.3, 62.3, 72.2, 76.9, 90.9, 91.4, 100.9,
104.8, 104.9, 110.4, 113.2, 113.4, 129.8, 143.9, 147.4, 149.5, 157.9, 167.5, 189.0.
HRMS (ESI): m/z calcd for C25H28O8 [M + H]+: 457.18569; found: 457.18602.
(–)-Dalpanol (4)
To a solution of ketone 28 (17.8 mg 0.0390 mmol) in MeOH (1.5 mL) was added aq 1 M HCl (0.39 mL) at rt. After
stirring for 1.5 h at 50 °C, the reaction was quenched by adding sat. aq NaHCO3. The products were extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by preparative TLC (hexane/EtOAc
1:1) to afford (–)-dalpanol (4; 14.3 mg, 89%) as a pale yellow solid. All physical data are fully identical with
those of the reported data.[14]
fluorobenzene 31
To a mixture of 3-fluorophenol (30; 6.2 mL, 68 mmol) and 3,4-dihydro-2H-pyran (9.2 mL, 0.10 mol) was added concd HCl (0.1 mL) at –35 °C. After warming to
rt over 1 h, the reaction was stopped by adding sat. aq NaHCO3. The products were extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was recrystallized from hexane to afford
fluorobenzene 31 (11.7 g, 88%) as colorless crystals; mp 49–50 °C (hexane); Rf
= 0.55 (hexane/EtOAc 9:1).
IR (neat): 2945, 2875, 1611, 1593, 1488, 1261, 1138, 1038, 975 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.57–1.64 (m, 1 H), 1.64–1.75 (m, 2 H), 1.80–1.94 (m, 2 H), 1.95–2.08 (m, 2
H), 3.58–3.68 (m, 1 H), 3.81–3.94 (m, 1 H), 5.40 (dd, J = 3.3, 3.3 Hz, 1 H), 6.68 (ddd, J = 8.3, 8.3 Hz, J
H,F = 2.2 Hz, 1 H), 6.79 (d, J
H,F = 10.9 Hz, 1 H), 6.83 (d, J = 8.3 Hz, 1 H), 7.21 (dd, J = 8.3 Hz, J
H,F =11.7 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 18.7, 25.1, 30.2, 62.0, 96.5, 104.2 (d, J
C,F = 24.0 Hz, 1 C), 108.3 (d, J
C,F = 21.0 Hz, 1 C), 112.1 (d, J
C,F = 3.0 Hz, 1 C), 130.0 (d, J
C,F = 9.0 Hz, 1 C), 158.4 (d, J
C,F = 12.0 Hz, 1 C), 163.5 (d, J
C,F = 243 Hz, 1 C).
19F NMR (565 MHz, CDCl3): δ = –115.0.
Anal. Calcd for C11H13FO4: C, 67.33; H, 6.68. Found: C, 67.47; H, 6.62.
phenol 33
To a solution of fluorobenzene 31 (105 mg, 0.535 mmol) in THF (1 mL) was added n-BuLi (1.64 M in hexane, 0.40 mL, 0.66 mmol) at –78 °C. After stirring for 1 h at
–78 °C, hexamethylphosphoric triamide (0.36 mL) in THF (1 mL) and prenyl bromide (111
mg, 0.745 mmol) in THF (1 mL) were added. After stirring for 1 h at –78 °C, the reaction
was stopped by adding H2O. The crude products were extracted with Et2O (3 ×), and the combined organic extracts were washed with H2O (2 ×) and brine, dried (Na2SO4), and concentrated in vacuo. The residue was dissolved in EtOH (1 mL), to which PPTS
(27.0 mg, 0.107 mmol) was added at rt. The reaction mixture was warmed to 60 °C and
stirred for 3 h. The reaction was stopped by adding sat. aq NaHCO3. The crude product was extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified flash column chromatography
(SiO2, hexane/EtOAc 10:1) to afford phenol 33 (88.9 mg, 92%) as a colorless oil; Rf
= 0.34 (hexane/EtOAc 6:1).
IR (neat): 3434, 2969, 2916, 1619, 1599, 1467, 1285, 1165, 1032, 782 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.74 (s, 3 H), 1.80 (s, 3 H), 3.39 (d, J = 7.2 Hz, 2 H), 5.25 (t, J = 7.2 Hz, 1 H), 5.37 (s, 1 H, OH), 6.59 (d, J = 8.1 Hz, 1 H), 6.63 (dd, J = 8.8 Hz, J
H,F = 8.9 Hz, 1 H), 7.03 (ddd, J = 8.8, 8.1 Hz, J
H,F = 7.4 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 17.8 22.0 (d, J
C,F = 4.5 Hz, 1 C), 25.8, 107.6 (d, J
C,F = 23.2 Hz, 1 C), 111.4 (d, J
C,F = 3.0 Hz, 1 C), 114.8 (d, J
C,F = 18.8 Hz, 1 C), 120.9, 127.3 (d, J
C,F = 10.5 Hz, 1 C), 135.2, 155.7 (d, J
C,F = 7.4 Hz, 1 C), 161.3 (d, J
C,F = 243.7 Hz, 1 C).
19F NMR (565 MHz, CDCl3): δ = –120.4.
HRMS (ESI): m/z calcd for C11H12FO [M – H]–: 179.08777; found: 179.08734.
ether 34
To a solution of phenol 33 (302mg, 1.68 mmol) in EtOH (16.6 mL) was added CuCl2 (113 mg, 0.850 mmol) and PdCl2 (45.0 mg, 0.254 mmol) at rt. After stirring for 6 h at 60 °C, the reaction mixture
was filtered through a Celite pad (washed with Et2O) and the filtrate was concentrated to half its volume in vacuo. The crude products
were added to aq 1 M NaOH. After extraction with hexane (3 ×), the combined organic
extracts were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by bulb-to-bulb distillation
(140 °C (oven temp)/20 mmHg) to afford ether 34 (220 mg, 74%) as a colorless oil; Rf
= 0.80 (hexane/EtOAc 6:1).
IR (neat): 2977, 2928, 1619, 1463, 1284, 1238, 1210, 1117, 1041, 754 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.44 (s, 6 H), 5.64 (d, J = 9.8 Hz, 1 H), 6.52–6.59 (m, 3 H), 7.02 (ddd, J = 11.5, 7.2 Hz, J
H,F = 7.2 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 27.9, 76.4, 107.3 (d, J
C,F = 21.0 Hz, 1 C), 110.0 (d, J
C,F = 18.2 Hz, 1 C), 112.1 (d, J
C,F = 3.1 Hz, 1 C), 115.1 (d, J
C,F = 4.7 Hz, 1 C), 128.9 (d, J
C,F = 10.3 Hz, 1 C), 130.7 (d, J
C,F = 2.5 Hz, 1 C), 154.0 (d, J
C,F = 7.0 Hz, 1 C), 158.6 (d, J
C,F = 248.1 Hz, 1 C).
19F NMR (565 MHz, CDCl3): δ = –126.5.
HRMS (APCI): m/z calcd for C11H10FO [M – H]–: 179.07212; found: 179.07276.
Epoxy Alcohol 35
To a solution of ether 34 (83.1 mg, 0.466 mmol) in Et2O (4.0 mL) and N,N,N′,N′-tetramethylethylenediamine (0.4 mL) was added s-BuLi (1.07 M in cyclohexane and hexane, 0.38 mL, 0.41 mmol) at –78 °C. After stirring
for 1 h at –78 °C, a solution of azeotropically dried (toluene, 1 mL 3 ×) epoxy ketone
19 (103 mg, 0.278 mmol) in Et2O (1.5 mL) was added. The reaction mixture was warmed to –50 °C over 1 h, and the
reaction was stopped by adding sat. aq NH4Cl. The crude products were extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by flash column chromatography
(SiO2, hexane/EtOAc 9:1) to afford epoxy alcohol 35 (152 mg, 85%) as a white amorphous solid; Rf
= 0.70 (hexane/EtOAc 2:1); [α]D
20 +24 (c 0.990, CHCl3).
IR (neat): 3497, 2954, 2931, 1619, 1513, 1405, 1259, 1222, 1117, 837 cm–1.
1H NMR (600 MHz, CDCl3): δ = –0.01 (s, 3 H), 0.02 (s, 3 H), 0.85 (s, 9 H), 1.40 (s, 3 H), 1.42 (s, 3 H),
3.24 (ddd, J = 6.9, 4.9, 2.7 Hz, 1 H), 3.48 (dd, J = 12.6, 2.7 Hz, 1 H), 3.66 (d, J
H,F = 1.5 Hz, 1 H, OH), 3.80 (dd, J = 12.6, 6.9 Hz, 1 H), 3.85 (s, 3 H), 3.87 (s, 3 H), 4.01 (dd, J = 4.9 Hz, J
H,F = 4.9 Hz, 1 H), 5.64 (d, J = 10.0 Hz, 1 H), 6.46 (d, J = 8.6 Hz, 1 H), 6.54 (d, J = 10.0 Hz, 1 H), 6.60 (d, J
H,F = 12.0 Hz, 1 H) 6.78 (dd, J = 8.6 Hz, J
H,F = 8.6 Hz, 1 H), 7.12 (d, J
H,F = 7.0 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = –5.3, –5.1, 18.4, 26.0, 28.0, 28.2, 56.3, 56.5, 59.6, 61.1, 61.8 (d, J
C,F = 7.5 Hz, 1 C), 71.9 (d, J
C,F = 3.9 Hz, 1 C), 76.8, 100.2 (d, J
C,F = 28.7 Hz, 1 C), 109.8 (d, J
C,F = 6.0 Hz, 1 C), 110.7 (d, J
C,F = 19.0 Hz, 1 C), 111.4 (d, J
C,F = 2.9 Hz, 1 C), 115.1 (d, J
C,F = 6.1 Hz, 1 C), 120.4 (d, J
C,F = 15.0 Hz, 1 C), 124.8 (d, J
C,F = 12.0 Hz, 1 C), 127.3 (d, J
C,F = 4.5 Hz, 1 C),131.1, 145.3 (d, J
C,F = 2.2 Hz, 1 C), 149.4 (d, J
C,F = 10.5 Hz, 1 C), 153.2 (d, J
C,F = 232.5 Hz, 1 C), 154.0, 156.4 (d, J
C,F = 250.5 Hz, 1 C).
19F NMR (565 MHz, CDCl3): δ = –125.7, –121.2.
HRMS (ESI): m/z calcd for C29H39F2O6Si [M + H]+: 549.24785; found: 549.24903.
Diol 37
To a solution of epoxy alcohol 35 (2.09 g, 3.81 mmol) in CH2Cl2 (70 mL) was added BF3·OEt2 (126 mg, 0.888 mmol) in CH2Cl2 (6.2 mL) at –15 °C. After stirring for 40 min, the reaction was stopped by adding
sat. aq NaHCO3. The crude product was extracted with CH2Cl2 (3 ×), and the combined organic extracts were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was dissolved in THF (76 mL), to which i-Bu2AlH (1.0 M in hexane, 11.4 mL, 11.4 mmol) was added at –78 °C. After stirring for
20 min at –78 °C, the reaction was stopped by adding sat. aq Rochelle’s salt. After
stirring for 40 min at rt, the crude products were extracted with EtOAc (3 ×). The
combined organic extracts were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by flash chromatography (SiO2, hexane/EtOAc 1:2) to afford diol 37 (1.85 g, 88%) as a white amorphous solid; Rf
= 0.42 (hexane/EtOAc 2:1); [α]D
20 +111 (c 1.60, CHCl3).
IR (neat): 3476, 2930, 2857, 1624, 1577, 1513, 1465, 1448, 1257, 1116 cm–1.
1H NMR (600 MHz, CDCl3): δ = –0.03 (s, 3 H), –0.01 (s, 3 H), 0.84 (s, 9 H), 1.39 (s, 3 H), 1.42 (s, 3 H),
2.72 (br s, 1 H, OH), 3.05 (br s, 1 H, OH), 3.28 (dd, J = 10.8, 8.2 Hz, 1 H), 3.42 (dd, J = 5.7, 3.5 Hz, 1 H), 3.51 (dd, J = 10.8, 4.1 Hz, 1 H), 3.81 (s, 3 H), 3.88 (s, 3 H), 4.02–4.17 (m, 1 H), 5.49 (dd,
J = 5.7 Hz, J
H,F = 2.5 Hz, 1 H), 5.64 (d, J = 10.0 Hz, 1 H), 6.44 (d, J = 8.4 Hz, 1 H), 6.47 (d, J
H,F = 11.1 Hz, 1 H), 6.57 (d, J = 10.0 Hz, 1 H), 6.89 (dd, J = 8.4 Hz, J
H,F =8.4 Hz, 1 H), 7.33 (d, J
H,F = 6.7 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = –5.4, –5.3, 18.3, 25.9, 27.7, 28.0, 43.9 (br s, 1 C), 56.0, 56.5, 65.7, 70.5,
74.2, 76.5, 99.3 (d, J
C,F = 30.0 Hz, 1 C), 109.6 (d, J
C,F = 18.8 Hz, 1 C), 112.0 (d, J
C,F = 2.8 Hz, 1 C), 113.7 (d, J
C,F = 4.9 Hz, 1 C), 114.3 (d, J
C,F = 15.0 Hz, 1 C), 115.4 (d, J
C,F = 5.7 Hz, 1 C), 121.0 (d, J
C,F = 13.5 Hz, 1 C), 127.6 (d, J
C,F = 6.1 Hz, 1 C), 130.8 (d, J
C,F = 2.1 Hz, 1 C), 144.9 (d, J
C,F = 2.5 Hz, 1 C), 148.6 (d, J
C,F = 10.0 Hz, 1 C), 153.2 (d, J
C,F = 6.9 Hz, 1 C), 155.5 (d, J
C,F = 248.4 Hz, 1 C), 156.1 (d, J
C,F = 237.5 Hz, 1 C).
19F NMR (565 MHz, CDCl3): δ = –132.1, –131.4.
HRMS (ESI): m/z calcd for C29H41F2O6Si [M + H]+: 551.26350; found: 551.26466.
Alcohol 38
To a solution of diol 37 (1.96 g, 3.56 mmol) in CH2Cl2 (12 mL) was added p-methoxybenzaldehyde dimethyl acetal (1.8 mL, 11 mmol) and PPTS (183 mg, 0.728 mmol)
at rt. After stirring for 3.5 h, the reaction was stopped by adding sat. aq NaHCO3. The crude product was were extracted with CH2Cl2 (3 ×), and the combined organic extracts were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was passed through a silica gel column (hexane/EtOAc
5:1) to afford the crude product contaminated with p-methoxybenzaldehyde dimethyl acetal and p-methoxybenzaldehyde (assessed by 1H NMR analysis). The mixture was dissolved in THF (18 mL), to which n-Bu4NF (1.0 M in THF, 10.7 mL, 10.7 mmol) was added at rt. After stirring for 1 h, the
reaction was stopped by adding phosphate buffer (pH 7). The crude products were extracted
with EtOAc (3 ×), and the combined organic extracts were washed with brine, dried
(Na2SO4), and concentrated in vacuo. The residue was purified by flash column chromatography
(hexane/EtOAc 2:1 to 1:1) to afford alcohol 38 (1.74 g, 89%) as a white amorphous solid; Rf
= 0.55 (hexane/EtOAc 1:1); [α]D
20 +156 (c 1.89, CHCl3).
IR (neat): 2935, 2916, 2849, 1616, 1514, 1465, 1251, 1209, 1092, 1036, 1009, 827 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.35 (s, 3 H), 1.38 (s, 3 H), 1.89 (br s, 1 H, OH), 3.44–3.53 (m, 2 H), 3.55
(dd, J = 3.0, 2.9 Hz, 1 H), 3.75 (s, 3 H), 3.78 (s, 3 H), 3.83 (s, 3 H), 4.55 (ddd, J = 7.7, 4.2, 2.9 Hz, 1 H), 5.53 (d, J = 3.0 Hz, 1 H), 5.62 (d, J = 9.9 Hz, 1 H), 5.93 (s, 1 H), 6.28 (d, J = 8.3 Hz, 1 H), 6.34 (d, J
H,F = 11.2 Hz, 1 H), 6.55 (d, J = 9.9 Hz, 1 H), 6.73 (dd, J = 8.3 Hz, J
H,F = 8.3 Hz, 1 H), 6.95 (d, J = 8.7 Hz, 2 H), 7.59 (d, J = 8.7 Hz, 2 H), 7.61 (d, J
H,F = 6.9 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 27.4, 27.8, 35.6 (d, J
C,F = 2.1 Hz, 1 C), 55.3, 55.8, 56.3, 64.1, 76.2, 76.3, 80.8, 98.8 (d, J
C,F = 30.0 Hz, 1 C), 102.0, 109.2 (d, J
C,F = 18.3 Hz, 1 C), 111.5 (d, J
C,F = 2.8 Hz, 1 C), 113.5 (d, J
C,F = 14.8 Hz, 1 C), 113.7, 113.8 (d, J
C,F = 4.7 Hz, 1 C), 115.2 (d, J
C,F = 5.4 Hz, 1 C), 118.1 (d, J
C,F = 13.1 Hz, 1 C), 126.9 (d, J
C,F = 5.9 Hz, 1 C), 127.5, 130.6, 130.7 (d, J
C,F = 2.1 Hz, 1 C), 144.4 (d, J
C,F = 2.4 Hz, 1 C), 148.2 (d, J
C,F = 10.1 Hz, 1 C), 152.8 (d, J
C,F = 6.7 Hz, 1 C), 154.5 (d, J
C,F = 248.3 Hz, 1 C), 155.6 (d, J
C,F = 237.5 Hz 1 C), 160.2.
19F NMR (565 MHz, CDCl3): δ = –132.3, –129.0.
HRMS (ESI): m/z calcd for C31H32F2O7Na [M + Na]+: 577.20083; found: 577.20079.
Ether 39
To a solution of azeotropically dried (toluene, 1 mL, 3 ×) alcohol 38 (28.6 mg, 0.0516 mmol) in toluene (1.1 mL) was added t-BuOK (16.2 mg, 0.144 mmol) at rt. The reaction mixture was refluxed for 80 min. After
cooling to rt, the reaction was stopped by adding sat. aq NH4Cl. The crude product was extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by preparative TLC (hexane/EtOAc
1:1) to afford ether 39 (20.3 mg, 74%) as a colorless oil; Rf
= 0.77 (hexane/EtOAc 1:1); [α]D
20 +44 (c 0.815, CHCl3).
IR (neat): 2974, 2834, 1619, 1515, 1480, 1463, 1345, 1249, 1216, 1199, 1158, 1113,
1045, 835 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.41 (s, 3 H), 1.46 (s, 3 H), 3.31 (s, 3 H), 3.41–3.43 (m, 1 H), 3.777 (s,
3 H), 3.781 (s, 3 H), 4.04 (d, J = 10.7 Hz, 1 H), 4.41–4.54 (m, 2 H), 5.50 (d, J = 2.6 Hz, 1 H), 5.71 (d, J = 9.9 Hz, 1 H), 5.86 (s, 1 H), 5.95 (s, 1 H), 6.42 (s, 1 H), 6.62 (d, J = 9.9 Hz, 1 H), 6.63 (d, J = 8.9 Hz, 1 H), 6.85 (d, J = 8.7 Hz, 2 H), 7.27 (dd, J = 8.9 Hz, J
H,F = 8.9 Hz, 1 H, overlapped with CHCl3 signal), 7.38 (d, J = 8.7 Hz, 2 H).
13C NMR (150 MHz, CDCl3): δ = 27.5, 27.9, 35.5 (d, J
C,F = 2.1 Hz, 1 C), 55.2, 55.3, 55.7, 68.2, 72.0, 76.3, 76.5, 100.9, 101.8, 109.7 (d,
J
C,F = 17.7 Hz, 1 C), 110.1, 111.5, 112.3 (d, J
C,F = 2.7 Hz, 1 C), 113.6, 114.8 (d, J
C,F = 5.4 Hz, 1 C), 119.8 (d, J
C,F = 12.8 Hz, 1 C), 127.8 (d, J
C,F = 6.1 Hz, 1 C), 127.8, 130.7, 131.4 (d, J
C,F = 2.1 Hz, 1 C), 142.2, 148.1, 149.2, 153.6 (d, J
C,F = 6.6 Hz, 1 C), 154.5 (d, J
C,F = 248.1 Hz, 1 C), 160.1.
19F NMR (565 MHz, CDCl3): δ = –131.5.
HRMS (ESI): m/z calcd for C31H31FO7Na [M + Na]+: 557.19460; found: 557.19289.
Alcohol 40
To a solution of azeotropically dried (toluene, 1 mL, 3 ×) ether 39 (189 mg, 0.354 mmol) in toluene (7.1 mL) was added i-Bu2AlH (1.0 M in hexane, 1.05 mL, 1.05 mmol) at 0 °C. After stirring for 1 h, the reaction
was stopped by adding sat. aq Rochelle’s salt. After stirring for 1.5 h at rt, the
crude product was extracted with EtOAc (3 ×), and the combined organic extracts were
washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by flash column chromatography
(hexane/EtOAc 4:1 to 2:1) to afford alcohol 40 (176 mg, 93%) as a white amorphous solid; Rf
= 0.55 (hexane/EtOAc 1:1); [α]D
20 +26 (c 1.26, CHCl3).
IR (neat): 3484, 2930, 1617, 1512, 1464, 1214, 1197, 1116, 1045, 822 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.46 (s, 3 H), 1.47 (s, 3 H), 1.96 (d, J = 7.7 Hz, 1 H), 3.35 (dd, J = 7.6, 4.1 Hz, 1 H), 3.57 (s, 3 H), 3.78 (s, 3 H), 3.81 (s, 3 H), 3.82–3.84 (m, 1
H), 3.97 (d, J = 10.5 Hz, 1 H), 4.15 (dd, J = 10.5, 7.7 Hz, 1 H), 4.31 (d, J = 11.1 Hz, 1 H), 4.40 (d, J = 11.1 Hz, 1 H), 5.02 (d, J = 7.6 Hz, 1 H), 5.68 (d, J = 10.0 Hz, 1 H), 6.40 (s, 1 H), 6.61 (d, J = 10.0 Hz, 1 H), 6.64 (d, J = 8.4 Hz, 1 H), 6.84 (d, J = 8.6 Hz, 1 H), 7.11 (s, 1 H), 7.16 (dd, J = 8.4 Hz, J
H,F = 8.4 Hz, 1 H), 7.21 (d, J = 8.6 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 27.9, 28.1, 44.2, 55.3, 55.8, 56.0, 65.4, 69.4, 70.2, 76.7, 77.0 100.2, 109.7
(d, J
C,F = 18.8 Hz, 1 C), 112.0, 112.3 (d, J
C,F = 2.8 Hz, 1 C), 113.1, 113.8, 115.1 (d, J
C,F = 5.2 Hz, 1 C), 118.6 (d, J
C,F = 13.7 Hz, 1 C), 128.8 (d, J
C,F = 6.1 Hz, 1 C), 129.4, 130.1, 130.8 (d, J
C,F = 2.1 Hz, 1 C), 143.2, 147.9, 148.7, 153.7 (d, J
C,F = 6.8 Hz, 1 C), 156.6 (d, J
C,F = 249.5 Hz, 1 C), 159.2; the missing signal was overlapped with CDCl3 signals.
19F NMR (565 MHz, CDCl3): δ = –130.4.
HRMS (ESI): m/z calcd for C31H33FO7Na [M + Na]+: 559.21025; found: 559.20992.
Ether 41
To a solution of azeotropically dried (toluene, 1 mL, 3 ×) alcohol 40 (604 mg, 1.13 mmol) in toluene (50.6 mL) and DMPU (5.6 mL) were added 15-crown-5
ether (221 μL, 1.12 mmol) and NaH (82.2 mg, 63% dispersion in mineral oil, 2.16 mmol)
at rt. After stirring for 2 h at 80 °C, the reaction was stopped by adding sat. aq
NH4Cl. The crude product was extracted with Et2O (3 ×), and the combined organic extracts were washed with H2O (3 ×) and brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by flash column chromatography
(hexane/EtOAc 5:1 to 3:1) to afford ether 41 (552 mg, 95%) as a white amorphous solid; Rf
= 0.67 (hexane/EtOAc 1:1); [α]D
20 +6.3 (c 0.865, CHCl3); [α]436
20 +17 (c 1.52, CHCl3).
IR (neat): 2931, 1610, 1512, 1458, 1248, 1195, 1146, 1116, 1091, 1033 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.37 (s, 3 H), 1.38 (s, 3 H), 3.47 (dd, J = 3.0, 2.8 Hz, 1 H), 3.71 (s, 3 H), 3.76 (s, 3 H), 3.81 (s, 3 H), 4.21 (d, J = 11.7 Hz, 1 H), 4.57 (d, J = 11.2 Hz, 1 H), 4.58–4.60 (m, 1 H), 4.61 (d, J = 11.2 Hz, 1 H), 4.73 (d, J = 3.0 Hz, 1 H), 4.83 (dd, J = 3.2, 2.8 Hz, 1 H), 5.49 (d, J = 10.0 Hz, 1 H), 6.30 (d, J = 8.3 Hz, 1 H), 6.36 (s, 1 H), 6.49 (s, 1 H), 6.66 (d, J = 10.0 Hz, 1 H), 6.86 (d, J = 8.3 Hz, 1 H), 6.90 (d, J = 8.6 Hz, 2 H), 7.31 (d, J = 8.6 Hz, 2 H).
13C NMR (150 MHz, CDCl3): δ = 27.7, 28.2, 35.7, 55.3, 55.7, 56.8, 66.2, 67.6, 69.7, 72.4, 76.0, 100.9, 108.5,
108.8, 109.7, 110.4, 110.7, 113.9, 116.7, 128.5, 129.4, 130.4, 130.6, 143.4, 148.5,
149.0, 149.8, 154.2, 159.3.
HRMS (ESI): m/z calcd for C31H32O7Na [M + Na]+: 539.20402; found: 539.20992; m/z calcd for C31H33O7 [M + H]+: 517.22208; found: 517.22191.
Alcohol 42 and Diol 43
To a solution of ether 41 (18.0 mg, 0.0348 mmol) and 2,6-di-tert-butylpyridine (40.2 mg, 0.210 mmol) in 1,4-dioxane (1.6 mL) and H2O (0.2 mL) was added DDQ (24.2 mg, 0.107 mmol) at rt. After stirring for 1 h at 50 °C,
the reaction was stopped by adding aq 10% Na2S2O3 and sat. aq NaHCO3. The crude materials were extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by preparative TLC (hexane/EtOAc
1:1) to afford alcohol 42 as a white amorphous solid (10.0 mg, 72%) and diol 43 (1.6 mg, 11%) as a white amorphous solid, respectively.
42
Rf
= 0.47 (hexane/EtOAc 2:3); [α]D
20 –51 (c 0.560, CHCl3).
IR (neat): 3461, 2973, 2930, 1511, 1462, 1221, 1194, 1146, 1090, 1016 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.37 (s, 3 H), 1.39 (s, 3 H), 1.97 (d, J = 3.2 Hz, 1 H, OH), 3.33 (dd, J = 3.3, 3.1 Hz, 1 H), 3.76 (s, 3 H), 3.78 (s, 3 H), 4.23 (d, J = 11.6 Hz, 1 H), 4.57 (dd, J = 11.6, 3.7 Hz, 1 H), 4.78 (dd, J = 3.7, 3.1 Hz, 1 H), 5.06, (d, J = 3.3, 3.2 Hz, 1 H), 5.52 (d, J = 10.0 Hz, 1 H), 6.35 (d, J = 8.3 Hz, 1 H), 6.39 (s, 1 H), 6.64 (s, 1 H), 6.65 (d, J = 10.0 Hz, 1 H), 7.02 (d, J = 8.3 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 27.8, 28.1, 37.7, 55.8, 56.7, 66.0, 66.6, 67.3, 76.1, 101.0, 108.9, 109.4,
109.7, 110.4, 113.6, 116.5, 128.8, 129.3, 143.4, 148.2, 149.1, 149.3, 154.3.
HRMS (ESI): m/z calcd for C23H25O6 [M + H]+: 397.16456; found: 397.16451.
43
Rf
= 0.39 (hexane/EtOAc 1:2); [α]D
20 –106 (c 0.515, CHCl3).
IR (neat): 3420, 2929, 1510, 1464, 1263, 1198, 1153, 1115, 1033, 755 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.37 (s, 3 H), 1.37 (s, 3 H), 2.46 (d, J = 3.4 Hz, 1 H, OH), 3.38 (s, 1 H, OH), 3.78 (s, 3 H), 3.78 (s, 3 H), 4.39–4.42 (m,
1 H), 4.51 (d, J = 11.8 Hz, 1 H), 4.54 (dd, J = 11.8, 2.7 Hz, 1 H), 5.03 (d, J = 3.4 Hz, 1 H), 5.51 (d, J = 10.0 Hz, 1 H), 6.33 (d, J = 8.3 Hz, 1 H), 6.39 (s, 1 H), 6.61 (d, J = 10.0 Hz, 1 H), 6.76 (s, 1 H) 6.97 (d, J = 8.3 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 27.8, 28.1, 55.8, 56.7, 64.5, 64.9, 68.6, 70.0, 76.2, 101.0, 108.9, 109.6,
109.6, 109.9, 113.3, 116.4, 128.9, 129.9, 143.8, 148.7, 149.4, 150.9, 154.7.
HRMS (ESI): m/z calcd for C23H25O7 [M + H]+: 413.15948; found: 413.16034.
(–)-Deguelin (2)
To a solution of alcohol 42 (10.0 mg, 0.0251 mmol) in DMSO (0.5 mL) was added IBX (29.2 mg, 0.104 mmol) at rt.
After stirring for 6.5 h, the reaction was stopped by adding sat. aq NaHCO3 and aq 10% Na2S2O3. The crude product was extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by preparative TLC (hexane/EtOAc
1:1) to afford (–)-deguelin (2; 8.1 mg, 82%) as a yellow amorphous solid; Rf
= 0.63 (hexane/EtOAc 1:1); [α]D
20 –46 (c 0.20, CHCl3).
IR (neat): 2966, 2926, 1673, 1597, 1579, 1513, 1442, 1345, 1274, 1214, 1198, 1112,
1094 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.39 (s, 3 H), 1.45 (s, 3 H), 3.77 (s, 3 H), 3.81 (s, 3 H), 3.84 (d, J = 4.1 Hz, 1 H), 4.19 (d, J = 12.0 Hz, 1 H), 4.64 (dd, J = 12.1, 3.1 Hz, 1 H), 4.92 (dd, J = 4.1, 3.1 Hz, 1 H), 5.56 (d, J = 10.1 Hz, 1 H), 6.45 (d, J = 8.7 Hz, 1 H), 6.45 (s, 1 H), 6.65 (d, J = 10.1 Hz, 1 H), 6.79 (s, 1 H), 7.75 (d, J = 8.7 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 28.2, 28.5, 44.4, 55.9, 56.3, 66.3, 72.4, 77.7, 100.9, 104.8, 109.1, 110.4,
111.5, 112.8, 115.8, 128.6, 128.7, 143.9, 147.4, 149.5, 157.0, 160.1, 189.2.
HRMS (ESI): m/z calcd for C23H23O6 [M + H]+: 395.14891; found: 395.14955.
Tephrosin (3) via Oxidation of Diol 43
Tephrosin (3) via Oxidation of Diol 43
To a solution of diol 43 (17.7 mg, 0.0429 mmol) in DMSO (0.5 mL) was added IBX (49.5 mg, 0.177 mmol) at rt.
After stirring for 5.5 h, the reaction was stopped by adding sat. aq NaHCO3 and aq 10% Na2S2O3. The crude product was extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by preparative TLC (hexane/EtOAc
1.1) to afford (–)-tephrosin (3; 14.5 mg, 82%) as a white amorphous solid; Rf
= 0.65 (hexane/ EtOAc 1:1); [α]D
20 –98 (c 0.20, CHCl3).
IR (neat): 3455, 1673, 1598, 1578, 1510, 1443, 1331, 1272, 1202, 1111, 1090, 1028
cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.39 (s, 3 H), 1.45 (s, 3 H), 3.73 (s, 3 H), 3.82 (s, 3 H), 4.42 (s, 1 H, OH),
4.50 (dd, J = 12.1, 1.1 Hz, 1 H), 4.57 (dd, J = 2.5, 1.1 Hz, 1 H), 4.63 (dd, J = 12.1, 2.5 Hz, 1 H), 5.56 (d, J = 10.1 Hz, 1 H), 6.47 (d, J = 8.7 Hz, 1 H), 6.48 (s, 1 H), 6.56 (s, 1 H), 6.60 (d, J = 10.1 Hz, 1 H), 7.73 (d, J = 8.7 Hz, 1 H).
13C NMR (150 MHz, CDCl3): δ = 28.3, 28.5, 55.9, 56.4, 63.9, 67.4, 76.3, 78.0, 101.1, 108.6, 109.1, 109.4,
111.1, 111.9, 115.4, 128.6, 128.8, 144.0, 148.4, 151.1, 156.7, 160.8, 191.4.
HRMS (ESI): m/z calcd for C23H22O7Na [M + Na]+: 433.12577; found: 433.12717.
Tephrosin (3) and 12a-epi-Tephrosin (44) via Oxidation of (–)-Deguelin (2) by IBX
Tephrosin (3) and 12a-epi-Tephrosin (44) via Oxidation of (–)-Deguelin (2) by IBX
To a solution of (–)-deguelin (2; 5.4 mg, 0.014 mmol) in DMSO (0.5 mL) was added IBX (15.4 mg, 0.0550 mmol) at rt.
After stirring for 5 h at 60 °C, the reaction mixture was warmed to 80 °C. The stirring
was continued for 14 h, and then the reaction was stopped by adding sat. aq NaHCO3 and aq 10% Na2S2O3. The crude products were extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by preparative TLC (hexane/EtOAc
1:1) to afford (–)-tephrosin (3; 2.4 mg, 44%) as a white amorphous solid and (+)-12a-epi-tephrosin (44; 2.4 mg, 44%) as a white solid.
(–)-Tephrosin (3)
[α]D
20 –81 (c 0.18, CHCl3).
(+)-12a-epi-Tephrosin (44)
(+)-12a-epi-Tephrosin (44)
Mp 208–210 °C; Rf
= 0.51 (hexane/EtOAc 1:1); [α]D
23 +2.6 × 102 (c 0.10, CHCl3).
IR (neat) 3450, 1690, 1594, 1575, 1504, 1447, 1266, 1109, 1088 cm–1.
1H NMR (600 MHz, CDCl3): δ = 1.45 (s, 3 H), 1.49 (s, 3 H), 2.75 (s, 1 H, OH), 3.85 (s, 3 H), 3.92 (s, 3
H), 4.41 (dd, J = 9.8, 4.4 Hz, 1 H), 4.50 (dd, J = 11.5, 9.8 Hz, 1 H), 4.66 (dd, J = 11.5, 4.4 Hz, 1 H), 5.63 (d, J = 10.1 Hz, 1 H), 6.43 (s, 1 H), 6.57 (d, J = 8.7 Hz, 1 H), 6.65 (d, J = 10.1 Hz, 1 H), 7.80 (d, J = 8.7 Hz, 1 H), 7.84 (s, 1 H).
13C NMR (150 MHz, CDCl3): δ = 28.1, 28.5, 55.9, 56.4, 61.7, 66.1, 77.8, 100.4, 109.0, 109.7, 112.2, 112.6,
113.6, 115.4, 129.3, 129.6, 143.8, 149.5, 151.1, 155.7, 159.7, 187.4; the missing
signal was overlapped with CDCl3 signals.
HRMS (ESI): m/z calcd for C23H21O6 [M – H2O + H]+: 393.13326; found: 393.13374.
Oxidation of (–)-Deguelin (2) by TBD and O2
Oxidation of (–)-Deguelin (2) by TBD and O2
To a solution of (–)-deguelin (2; 7.0 mg, 0.018 mmol) in DMSO (0.6 mL) and Cu2O (0.6 mg, 0.004 mmol) was added 1,5,7-triazabicycro[4.4.0]dec-5-ene (4.5 mg, 0.032
mmol) at rt under O2. After stirring for 4.5 h, the reaction was stopped by adding sat. aq NH4Cl. The crude products were extracted with EtOAc (3 ×), and the combined organic
extracts were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by preparative TLC (hexane/EtOAc
1:1) to afford (±)-tephrosin (3; 3.9 mg, 53%) as a white amorphous solid and (±)-12a-epi-tephrosin (44; 2.0 mg, 27%) as a white solid.
Oxidation of (–)-Deguelin (2) by K2Cr2O7
Oxidation of (–)-Deguelin (2) by K2Cr2O7
To a solution of (–)-deguelin (2; 4.8 mg, 0.012 mmol) in AcOH (0.25 mL) and H2O (0.08 mL) was added K2Cr2O7 (5.3 mg, 0.018 mmol) at 60 °C. After stirring for 30 min, the reaction was stopped
by adding sat. aq NaHCO3 and aq 10% Na2S2O3. The crude products was extracted with EtOAc (3 ×), and the combined organic extracts
were washed with brine, dried (Na2SO4), and concentrated in vacuo. The residue was purified by preparative TLC (hexane/EtOAc
3:2, 2 ×) to afford (–)-tephrosin (3; 4.7 mg, 94%) as a white amorphous solid; [α]D
20 –84 (c 0.23, CHCl3).
