Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608062
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Ginger (Zingiber officinale) Extract Promotes Telomere Shortening and Induces Cellular Senescence in A549 Lung Cancer Cells

N Kaewtunjai
1  Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
,
W Pompimon
2  Department of Chemistry, Faculty of Science and Center of Innovation in Chemistry, Lampang Rajabhat University, Lampang, Thailand
,
W Tuntiwechapikul
1  Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 
 

    Cellular senescence caused by telomere shortening is considered a tumor suppressor mechanism.1 However, more than 85% of cancers maintain their telomeres by reactivating telomerase to escape the replicative senescence.2 Previously, we reported that the crude ethyl acetate fraction of Zingiber officinale extract (ZOE) suppressed the expression of the hTERT and c-Myc proteins, leading to the reduced telomerase activity in A549 lung cancer cells.3 In this report, we demonstrate that long-term treatment with subcytotoxic doses of ZOE, which allowed A549 cells to proliferate normally, induced telomere shortening corresponding with ZOE-induced telomerase suppression. The telomere shortening consequently induced cellular senescence in these cells, which was shown by the increase in the senescence associated β-galactosidase positive cells and the reduction in clonogenicity. In order to find the telomerase suppressors in ZOE, we employed assay-guided fractionation and GC/MS analysis to identify the chemical components. We found that the major compounds in all active subfractions contained paradols and shogaols of different chain lengths. We have concluded that the telomerase suppressors in ZOE are likely to be paradols and shogaols. Altogether, these results suggest ZOE could potentially be used as a dietary phytochemical for cancer prevention.

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    [2] Kim NW, Piatyszek MA, Prowse KR, et al. Science 1994; 266: 2011 – 2015.

    [3] Tuntiwechapikul W, Taka T, Songsomboon C, Kaewtunjai N, Imsumran A, Makonkawkeyoon L, Pompimon W, Lee TR. J Med Food 2010; 13: 1347 – 1354.


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