Keywords
ADAMTS13 - colitis - Crohn's disease - thrombosis - VWF
Introduction
Inflammatory bowel disease (IBD), which includes Crohn's disease and ulcerative colitis
(UC), are diseases of chronic intestinal inflammation, manifesting in relapsing and
remitting (often bloody) diarrhea, as well as debilitating abdominal pain. IBD affects
1.6 million individuals in the United States[1] and is rising in incidence. The precise pathogenesis of IBD is unknown; it is thought
to arise from a combination of genetic predisposition and a dysregulated inflammatory
response to an environmental, likely microbial, trigger.[2]
Interestingly, patients with IBD have a 3-fold increased risk of developing thromboembolism
compared with age-matched controls, and this risk rises 15-fold with worsening disease
activity[3] (reviewed in Zitomersky et al[4]). Deep vein thrombosis (DVT) and pulmonary embolism (PE) are the most common thrombotic
events, but arterial events,[5] including cardiovascular and mesenteric ischemia, are also more prevalent in people
with IBD.[6] Some postulate that thrombosis may even play a role in IBD pathogenesis.[7]
[8]
[9] Increasing evidence links inflammation and thrombosis in multiple chronic inflammatory
states, including IBD (reviewed in Esmon[10] and Feys et al[11]).
Leukocyte rolling, adhesion, and transmigration are hallmarks of inflammation. Adhesion
molecules on leukocytes and endothelial cells mediate this process. In fact, antibody
blockade of adhesion receptors involved in trafficking of leukocytes to the gut, such
as integrins, are used to treat IBD.[12] The adhesion molecule, von Willebrand Factor (VWF), is a large multimeric glycoprotein.
In its ultra-large form (ULVWF), VWF is stored in the Weibel-Palade bodies of the
endothelium, as well as in α-granules of platelets. ULVWF is released from endothelium
upon activation by inflammatory signals or with hypoxia.[13] VWF release leads to the initial adhesion of platelets and leukocytes to the vessel
wall, a first step in initiating both inflammation and thrombosis.[13]
[14] Leukocytes adhere to VWF, or to platelets recruited by the released VWF, directly
through P-selectin glycoprotein ligand and β2-integrins.[15] ULVWF multimers are extremely biologically active, as they form stronger bonds with
platelet GPIb.[16] A disintegrin and metalloproteinase with thrombospondin type I repeats—motif 13
(ADAMTS13) decreases platelet and leukocyte adhesion, as well as VWF-platelet string
formation, by specifically cleaving hyperactive ULVWF multimers under conditions of
fluid shear stress.[17] Mice that are completely deficient in ADAMTS13 (ADAMTS13−/−) have a proinflammatory and prothrombotic phenotype.[18] Mice that express ADAMTS13, which contains a human mutation responsible for reducing
enzymatic activity, also have a prothrombotic phenotype,[19] indicating that even reduction in ADAMTS13 activity[20] is deleterious. Additionally, diseases of chronic inflammation associated with thrombosis
have higher plasma VWF, and reduced ADAMTS13 levels and activity,[11] which could exacerbate a prothrombotic or proinflammatory state. Thus, in addition
to being antithrombotic, ADAMTS13 is increasingly recognized as an anti-inflammatory
mediator.
Circulating VWF is elevated in IBD patients[11]
[21] and rises with worsening colitis disease activity,[22] which suggests increased endothelial activation. In plasma from IBD subjects, ADAMTS13
levels and activity are reduced, when compared with healthy control plasma.[11] Therefore, ADAMTS13 deficiency, or acquired antibody-mediated ADAMTS13 inhibition,
could contribute to disease severity and increased incidence of thrombosis in this
population. Several cases of patients suffering from both thrombotic thrombocytopenic
purpura (thrombotic microangiopathy due to very low ADAMTS13 activity) and colitis
were reported.[23]
Mechanisms of platelet recruitment in colitis are not completely understood. In dextran
sodium sulfate (DSS) colitis, platelets and leukocytes accumulate in venules of the
inflamed colon.[24] They bind endothelium and leukocytes on to the vessel wall. The presence of these
adherent platelets and leukocytes in colonic vessels correlates with disease severity.[24] ULVWF binds platelets and recruits leukocytes in thrombosis and inflammation, but
its impact has not been extensively studied in colitis.
We evaluated the impact of ADAMTS13 deficiency on colitis induced by a chemical (DSS)
in drinking water. We compared disease severity of DSS colitis in wild-type (WT) mice
that were given either ADAMTS13 or a vehicle buffer. Our results indicate that ADAMTS13
deficiency leads to the accumulation of VWF-rich thrombi in colonic submucosal vessels,
increasing inflamed colonic tissue, and a worse colitis phenotype. Moreover, treatment
of WT mice with recombinant human ADAMTS13 (rhADAMTS13) ameliorates DSS colitis. Thus,
ADAMTS13 is an important modifier in the disease.
Materials and Methods
Animals
ADAMTS13−/− [25] and WT mice were on a C57BL/6J background. All animals were 6- to 8-week-old males,
weight-matched, in-house bred, with the majority derived from heterozygous by heterozygous
crosses, and the remaining from in-house bred WT C57BL/6J. As the two genotypes compared
were siblings raised in the same cage, it can be assumed that their gut microbiota
were highly similar. Rag2−/−
, Rag2−/−Il10rb−/−
, Il10rb−/−
, Was−/−
, and Was−/−Il4−/−
mice were on 129 SvEv background.[26]
[27] All experimental procedures were reviewed and approved by the Institutional Animal
Care and Use Committee of Boston Children's Hospital.
Dextran Sodium Sulfate Colitis Model
Acute DSS colitis was induced by administration of DSS salt (3% wt/vol, mol wt: 36–50
kDa; MP Biomedicals) in autoclaved drinking water for 10 days as described.[28] Fresh DSS was administered on days 3 and 6. Daily weight and clinical colitis scores
were measured using an established scoring system based on percentage weight loss
(<10% = 1, >10% = 2, >20% = 3), onset of diarrhea (1), bloody diarrhea (2), hunched
posture (1), and rectal prolapse (1) for a maximum score of 7.[28]
Recombinant Human ADAMTS13 Treatment
rhADAMTS13 was provided by F. Scheiflinger and H. Rottensteiner of Baxter Bioscience.
Treatment with rhADAMTS13 (or saline vehicle) by retro-orbital intravenous injections
was done on days 3 to 10 at a dose of 3,460 U/kg every 24 hours, a concentration shown
to decrease leukocyte recruitment in myocardial ischemia reperfusion injury.[29] This concentration was chosen due to our experience with a 10-fold lower dose of
this human protein only partially dismantling mouse “VWF platelets strings” observed
in activated venules by intravital microscopy. A 10-fold higher dose shows no toxicity
in mice (Friedrich Scheiflinger, PhD, oral communication).
Analysis of Peripheral Blood
Blood was collected via the retro-orbital sinus into EDTA-coated capillary tubes and
was analyzed by a Hemavet 950FS (Drew Scientific) for complete blood counts.
Plasma VWF, IL-6, IL-10, Soluble P-selectin, and Thrombin–Antithrombin Complexes
Plasma VWF levels were quantified by enzyme-linked immunosorbent assay (ELISA) on
day 10.[30] VWF levels in plasma of WT or ADAMTS13−/− mice were calculated and shown as fold increase over pooled plasma VWF levels of
untreated WT or untreated ADAMTS13−/− mice, respectively. Interleukin (IL)-6 (Biolegend), IL-10 (R&D Systems), soluble
P-selectin (sPsel; R&D Systems), and thrombin–antithrombin (TAT) complexes (Abcam)
were measured by ELISA according to the manufacturer's instructions.
Human Tissue Specimens
Anonymous human colon tissue specimens were obtained from the Pediatric Inflammatory
Bowel Disease Biospecimen Repository at Boston Children's Hospital. This repository
was approved by the Institutional Review Board of Boston Children's Hospital (protocol
number: IRB-P00000529). Prior to specimen deposit, informed written consent was obtained
from study subjects and/or their legal guardians.
Tissue Preparation and Analysis
Mice were anesthetized with isoflurane and terminally bled from the retro-orbital
sinus. Entire spleens were harvested and weighed. Colon length was measured from anal
verge to cecum. Then, colons were cut longitudinally and Swiss rolled so that the
entire colon could be evaluated. Colons were fixed in zinc fixative (100 mM Tris-HCl
containing 37 mM zinc chloride, 23 mM zinc acetate, and 3.2 mM calcium acetate). Paraffin-embedded
sections were stained with hematoxylin and eosin (H&E) and scored for colitis severity
using an established scoring system[31] by an individual blinded to the study groups. Human colon tissue biopsies were embedded
in optimal cutting temperature, snap frozen, cryostat sectioned, and fixed in zinc,
as described earlier.
Immunostaining and Fluorescence Microscopy
Tissue sections were washed with phosphate-buffered saline (PBS) and permeabilized
(0.1% Triton X-100, 0.1% sodium citrate) for 10 minutes at 4°C. Samples were blocked
with 3% (wt/vol) bovine serum albumin (BSA) for 90 minutes at 37°C, rinsed, and incubated
overnight at 4°C or for 1 hour at 37°C in primary antibody dilution buffer containing
0.3% BSA, rabbit antihuman VWF (DAKO, 1:500), and rat antimouse CD31 (1:250, Biolegend).
After several washes, samples were incubated for 2 hours at room temperature in antibody
dilution buffer containing Alexa Fluor-conjugated secondary antibodies in 0.3% BSA
in PBS: goat anti-rat immunoglobulin G (IgG) (Alexa555, 2 μg/mL; Biolegend), donkey
anti-rabbit IgG (Alexa488, 1.5 μg/mL; Biolegend), or donkey anti-sheep IgG (Alexa568,
2 μg/mL; Invitrogen). DNA was counterstained with 1 μg/mL Hoechst 33342 and slides
were coverslipped with Fluoromount gel (Electron Microscopy Sciences). Fluorescent
images were acquired using an Axiovert 200 wide field fluorescence microscope (Zeiss)
in conjunction with an Axiocam MRm monochromatic CCD camera (Zeiss) and analyzed with
Zeiss Axiovision software. All channels were acquired in grayscale and pseudo colored
using Zeiss Axiovision or ImageJ software (National Institutes of Health). VWF immunofluorescent
intensity in colon tissue was quantified by FITC intensity at the same exposure time
on the same day divided by DNA area, which stained all nuclei; therefore, it estimated
entire tissue area in five separate 20× images.
Statistics
Data are presented as mean ± standard error of mean and analyzed using Student's t-test or Mann-Whitney U-test with median ± IQR unless otherwise noted. Data were considered significant when
p-values were less than 0.05. For comparison of platelet count and weight loss after
DSS colitis induction between WT and ADAMST13−/− mice ([Fig. 1B]), an analysis of covariance was used with a 95% confidence interval to determine
if there was a difference between the two slopes.
Fig. 1 Effects of ADAMTS13 deficiency on platelet count and colonic thrombosis. (A) Platelet counts for WT and ADAMTS13−/− mice without colitis did not differ. With colitis, there was significant thrombocytosis
in WT mice, while in ADAMTS13−/− mice platelet count varied more widely but had a similar mean to baseline. (B) Platelet counts trended to be lower in mice with more weight loss, an indicator
of worse colitis, and tended to decrease more in ADAMTS13−/− mice with colitis (slopes p = 0.12). (C) Colons from WT and ADAMTS13−/− mice were fluorescently stained for VWF (green), PECAM-1 (red), and DNA (blue). In
WT mice, we observed increased VWF expression mostly within the vessel wall with likely
some VWF-positive platelets in the lumen. In contrast, in most ADAMTS13−/− mice, we found a completely occlusive VWF-rich thrombus in a submucosal vessel. Entire
Swiss-rolled sections of colon were inspected for thrombi. We found one or more VWF-rich
thrombi in most ADAMTS13−/− colon sections and none in WT colons with colitis. In (A), mean and SEM are shown. In (C), median ± IQR values are presented.*p < 0.05, **p < 0.005, ***p < 0.0005.
Results
DSS-Induced Colitis Is More Severe in ADAMTS13-Deficient Mice than in WT
To address the role of ADAMTS13 in colonic inflammation, we chemically induced colitis
in littermate ADAMTS13−/− and WT mice. Initial body weight did not differ between ADAMTS13−/− and WT mice, nor did either genotype have diarrhea at baseline. In three separate
experiments, ADAMTS13−/− mice (n = 15) with DSS-induced colitis lost more weight compared with WT (n = 17) on days 9 (p < 0.005) and 10 (p < 0.0005), when colitis is most severe ([Fig. 2A]). Clinical colitis scores were also higher on days 8 to 10 ([Fig. 2B]; p < 0.005, p < 0.05, p < 0.0005, respectively). Higher scores in ADAMTS13−/− mice were due to more weight loss, earlier onset of bloody diarrhea, and hunched
body habitus. In acute colitis, hemoglobin concentration decreases due to blood loss
in stool. Hemoglobin concentration did not differ significantly at baseline prior
to colitis induction, suggesting that ADAMTS13 deficiency did not result in clinically
significant bleeding without intestinal mucosal injury ([Fig. 2C]). ADAMTS13−/− mice developed worse anemia (p < 0.005) compared to WT mice with colitis ([Fig. 2C]).
Fig. 2 ADAMTS13 deficiency aggravates DSS colitis in mice. (A) Percent of original body weight in WT and ADAMTS13−/− mice. ADAMTS13−/− mice had significantly more weight loss on days 9 to 10 when their disease was most
severe. (B) ADAMTS13−/− mice have a more severe colitis phenotype based on clinical colitis scores on days
8 to 10. (C) Hemoglobin concentration in WT and ADAMTS13−/− mice without colitis is not significantly different at baseline. With colitis, both
WT and ADAMTS13−/− mice became anemic, but the ADAMTS13−/− mice became more anemic suggesting more severe disease. (D) H&E-stained colon sections from WT and ADAMTS13−/− mice without colitis show normal crypt architecture with neatly aligned crypts (stars)
whose bases are adjacent to the muscularis mucosa (black arrows), abundant clear mucous-filled
goblet cells, preserved epithelial barrier with epithelial cells and their basolateral
nuclei (diamonds), and no mucosal, submucosal, or muscularis mucosa (M) edema. Representative
sections of WT mouse colon sections after DSS colitis for 10 days showing distortion
of crypt architecture (branching of the normally test tube–shaped crypts), loss of
goblet cells previously seen within crypts, and increased leukocytes infiltrating
the lamina propria between the crypts (white starbursts). There are also more leukocytes
and edema in the submucosa, which widens the submucosal space (double-headed arrow).
An intact epithelial barrier remained protecting the gut from luminal contents (L).
On the right panel, with increased disease severity, there was complete loss of crypt
architecture and increased leukocyte infiltration, but the epithelial barrier was
maintained in WT. Representative sections of ADAMTS13−/− colon sections show similar features as wild type but had more severe inflammation
with increased crypt distortion in the middle panel and then complete loss of crypt
architecture, increased leukocyte infiltration, a more widely expanded submucosal
space, and transmural inflammation and ulceration with a ragged disorganized epithelial
barrier. Bar = 50 μm. (E) Colon length was slightly shorter in ADAMTS13−/− mice than in WT at baseline. With colitis, ADAMTS13−/− mice had shorter colons than WT, suggesting more edema and inflammation. (F) Spleen weight did not differ at baseline between ADAMTS13−/− and WT but significantly increased in both genotypes with colitis. (G) Histologic scoring of H&E-stained colon sections from WT and ADAMTS13−/− mice showed more severe disease in ADAMTS13−/− mice. (H–K) Circulating total leukocytes and subsets were measured at baseline and on day 10
of colitis. (H) Leukocyte counts did not differ at baseline but decreased more in ADAMTS13−/− mice with colitis. (I) Neutrophils increased in WT mice with DSS colitis but not in ADAMTS13−/− mice with colitis. (J) Lymphocytes did not differ between genotypes at baseline or with colitis. (K) Monocytes increased in both genotypes with colitis. In all graphs, mean and SEM
are shown. *p < 0.05, **p < 0.005, ***p < 0.0005.
With colonic inflammation, the colon becomes edematous and shortens.[28] ADAMTS13−/− mice had slightly shorter colons compared with WT mice, even without colitis ([Fig. 2E], p < 0.005). Because of this slight difference, we looked for microscopic inflammation
in H&E-stained colon sections from untreated ADAMTS13−/− mice, but we found no evidence of inflammation ([Fig. 2D]). After induction of DSS colitis, colons from ADAMTS13−/− were significantly shorter compared with WT-treated mice (p < 0.005) as well as untreated ADAMTS13−/− mice, suggesting severe colitis ([Fig. 2E]). Spleen weight is a marker of systemic inflammation in colitis mouse models. Spleen
weight increased significantly in both WT and ADAMTS13−/− mice with colitis (p < 0.0005). However, it did not differ between ADAMTS13−/− and WT mice ([Fig. 2F]). The development of anemia, colon shortening, and increased spleen weight confirmed
the induction of severe colitis in both genotypes of mice. Entire H&E-stained Swiss-rolled
colons were evaluated for histologic colitis severity using an established scoring
system.[31] DSS-treated ADAMTS13−/− colons had more histologic evidence of inflammation compared with WT mice ([Fig. 2G], p < 0.05) with representative sections shown in [Fig. 2D].
Blood counts revealed lower total circulating leukocytes in DSS-treated ADAMTS13−/− mice compared with WT mice ([Fig. 2H]). Circulating leukocyte counts did not differ at baseline ([Fig. 2H]). Neutrophils increased in WT mice with colitis but were unchanged in ADAMTS13−/− mice with colitis ([Fig. 2I]). Lymphocytes in WT mice with colitis were unchanged, with the propensity to be
lower in ADAMTS13−/− mice, compared with WT mice ([Fig. 2J]). Monocytes rose significantly in WT and ADAMTS13−/− mice with colitis, but did not differ between the two genotypes ([Fig. 2K]). No significant differences in plasma were observed in the proinflammatory cytokine
IL-6, or the anti-inflammatory cytokine IL-10, TAT complexes, or sPsel between WT
and ADAMTS13−/− mice with colitis ([Fig. S1A–D], supplementary figure available in the online version only). These results suggest
that ADAMTS13-deficiency favors the local accumulation of inflammatory cells in colonic
vessels and tissue, likely without affecting systemic inflammation.
ADAMTS13−/− Mice Drop Their Platelet Count and Develop Colonic Submucosal Thrombi with Colitis
Thrombocytosis is a marker of colonic inflammation, and is seen in colitis in mice
as well as humans.[8]
[32] We evaluated platelet counts in WT and ADAMTS13−/− mice before and after colitis initiation. Platelet counts in WT and ADAMTS13−/− mice did not differ prior to colitis initiation ([Fig. 1A]). WT mice demonstrated the typical pattern of significant thrombocytosis with DSS
colitis (p < 0.0005), whereas ADAMTS13−/− mice had no significant difference in platelet count after the development of colitis
(p = 0.12). In fact, ADAMTS13−/− with the most severe colitis phenotype (indicated by more weight loss) tended to
be more thrombocytopenic ([Fig. 1B]). This finding led us to wonder whether in ADAMTS13−/− mice platelets were consumed in colonic thrombi. Therefore, we sectioned entire Swiss-rolled
colons, and immunofluorescently stained them for VWF and PECAM-1 (CD31) to identify
vessels. In a majority of ADAMTS13−/− mice with colitis, we found one or more submucosal vessel(s) completely occluded
by a VWF-rich thrombus, not detected in WT ([Fig. 1C], p < 0.05). Notably, in colons from WT and ADAMTS13−/− mice without colitis, we found no submucosal thrombi. Taken together, these results
suggest that, through accumulation of ULVWF in the colonic vessels, ADAMTS13 deficiency
worsens colitis by inducing local inflammation and thrombosis.
WT and ADAMTS13−/− Mice Show Prominent Subendothelial VWF Staining in Inflamed Colonic Tissue
To characterize VWF in murine colitis, we quantified plasma VWF at baseline, and at
day 10 of colitis. As we expected, plasma VWF increased in DSS colitis in both WT
and ADAMTS13−/− mice. Interestingly, VWF increased significantly more in WT than in ADAMTS13−/− mice ([Fig. 3A]), perhaps indicating that more ULVWF was retained in the ADAMTS13−/− mouse colons.
Fig. 3 Colitis causes massive VWF release. (A) WT (n = 13) and ADAMTS13−/− (n = 9) mice had increased plasma VWF with colitis. The plot displays the fold increase
over genotype mean without and with DSS treatment. (B) Healthy WT and ADAMTS13 mouse colons and colons from both genotypes after colitis
were immunofluorescently stained for VWF, PECAM-1, and DNA. In WT mice, there was
minimal VWF (green) staining seen only within vessel walls. There was not significantly
more VWF stain in ADAMTS13−/− mouse sections without colitis. With colitis we saw an increase in VWF-positive staining
which was more significant in ADAMTS13−/− mice likely due to decreased clearance of VWF from tissue. (C) Using Image J software, we quantified VWF immunofluorescence intensity divided by
DNA area (to represent colon tissue area captured on the image). We confirmed higher
VWF intensity in ADAMTS13−/− mice with colitis compared with ADAMTS13−/− without colitis. Furthermore, ADAMTS13−/− mice with colitis had significantly more VWF staining intensity compared with WT
mice with colitis. Notably, there was no difference in WT and ADAMTS13−/− VWF signal intensity at baseline. There was a propensity for VWF intensity to increase
with colitis in WT mice (p = 0.07). Mean and SEM are shown. **p < 0.005.
To test our hypothesis, we immunofluorescently stained colons from WT and ADAMTS13−/− mice without colitis for VWF. In healthy WT mice, VWF staining was in a dotted pattern
and closely associated with PECAM-1-positive endothelium, suggesting it was still
contained in endothelial Weibel-Palade bodies ([Fig. 3B]). In contrast, both WT and ADAMTS13−/− mice with colitis had increased areas of VWF-stained colonic tissue extending beyond
PECAM-1-positive vessels into the subendothelial space ([Fig. 3B]). Next, the colonic sections were assessed for VWF immunofluorescent intensity by
an observer blinded to the mouse genotype and treatment. There was more intense VWF
staining in colonic tissue from ADAMTS13−/− mice compared with WT mice with colitis (p = 0.0023, [Fig. 3C]). VWF intensity in ADAMTS13−/− mice increased significantly over baseline (p = 0.0012), while WT showed only a propensity to increase VWF tissue intensity (p = 0.07), indicating that ADAMTS13 is clearing the released VWF from the sites of
inflammation. VWF intensity in colonic tissue did not significantly differ at baseline
between WT and ADAMTS13−/− mice (p = 0.43). This apparent retention of released VWF in inflamed tissue, along with small
thrombi formation, may explain the relatively lower increase of plasma VWF in colitis
in the ADAMTS13−/− mice compared with WT mice ([Fig. 3A]).
Increased VWF Release in Colon Tissue Is Seen in Other Murine Colitis Models and in
Human Colitis Specimens
In the DSS colitis model, acute colonic injury leads to the development of inflammation,
mainly a Th1-mediated response,[33] which is immunologically distinct from chronic colitis models, and relapsing and
remitting human disease. Despite these distinctions, we hypothesized that increased
colonic VWF release may also occur in additional immune-mediated colitis models, as
well as in human colitis. Indeed, we found prominent VWF staining in colitic tissue
from several chronic colitis models. Interstitial VWF release seemed more pronounced
in models with a more severe colitis phenotype. It is difficult to generalize among
these models with various immune defects leading to colitis. However, we can make
a few comparisons. Most notably, the WT CD4 T cell transfer into Rag2
−/− model has a mild colitis phenotype without any weight loss 4 weeks posttransfer,
and minimally increased colitis scores.[26] In this model, there was minimally increased colonic VWF staining compared with
control WT colons and staining was contained within vessel walls ([Fig. 4]). Additionally, we saw slightly increased VWF staining in the IL10 receptor β (Il10rb)−/− mice which have a mild phenotype with colitis development after 15 weeks.[34] In contrast, when these two genotypes are combined, WT CD4 T cell transfer into
Rag2
−/− Il10rb
−/− mice has 20% weight loss 4 weeks posttransfer and colitis scores that are six times
worse than the WT CD4 T cell transfer into Rag2
−/−.[26] Colons from these more severely affected mice showed extensive and intense VWF staining
([Fig. 4]). Mice deficient in Wiskott Aldrich Syndrome (WAS) protein develop severe colonic
inflammation by the age of 12 to 16 weeks with mixed Th1- and Th2-type responses.[27] Disease in these mice is partially driven by IL4 as deletion of IL4 improves disease
in Was
−/−
Il4−/−
mice.[27] Similar to disease severity, VWF staining in colonic tissues from Was
−/− mice is more pronounced compared with Was
−/−
Il4−/−
mice, which corroborates our hypothesis ([Fig. 4]).
Fig. 4 Mouse models of chronic colitis show signs of VWF release in affected colonic tissue
with anti-VWF fluorescent staining. WT mice colon tissue sections (top) stained for
VWF (green) and DNA (blue) show minimal VWF-positive staining with faint expression
within vessel walls. Colon sections from T cell transfer and chronic colitis models
showed increased VWF staining dependent on the severity of the phenotype. WT CD4(+)
T cell transfer into Rag2−/−
and Il10rb−/−
mice has a less severe phenotype and show less VWF staining. When WT T cells are
transferred into Rag2−/−Il10rb−/−
mice, these have a more severe phenotype and show increased VWF within and extending
beyond vessels seen on the left and within neo-vessels under ulcerated tissue in the
right image. Wiskott-Aldrich syndrome (WAS−/−) protein-deficient mice develop colonic inflammation between 12 and 16 weeks which
is partially driven by IL-4. A double knockout of WAS and IL4 (WAS−/−IL4−/−) has a milder phenotype. Less VWF is seen in the milder WAS−/−/IL4−/− compared with the WAS−/−. Bar = 50 μm.
In human inflamed colonic tissue, similar to DSS colitis and the chronic colitis models
mentioned earlier, we observed increased VWF release at sites of marked inflammation
in comparison to healthy sites in 12 human colitis biopsy or colon resection specimens
from individuals with IBD ([Fig. 5]). Staining was both intravascular and extravascular with VWF extending into colon
tissue ([Fig. 5A]). Together, these results suggest that even without ADAMTS13-deficiency, colitis
induces the release of VWF in inflamed colonic vessels and tissue, which increases
with the severity of the disease.
Fig. 5 Elevated VWF staining was seen in human colitis colon tissue. (A) Human colon tissue stained for VWF (green) and DNA (blue) shown at various magnifications.
The top row is healthy colonic tissue with minimal VWF staining only within vessels.
The second and third rows are samples from two different subjects with active ulcerative
colitis (UC) and reveal increased VWF release surrounding vessels and deeper in colonic
tissue. (B) A submucosal vessel (likely representing a thrombus) from a patient with UC cut
longitudinally showing retained VWF (green) inside the vessel surrounded by endothelial
cells stained for PECAM-1 (red). DNA (blue) stains all cells and shows the surrounding
muscularis mucosa. Bar = 50 μm.
Administration of rhADAMTS13 to WT Mice with DSS Colitis Reduces Clinical Colitis
Severity
VWF accumulates at inflamed sites in colitis, as documented earlier ([Figs. 3B] and [4]). VWF promotes leukocyte recruitment and transmigration,[15]
[35] thus aggravating inflammation. Since ADAMTS13 can clear released VWF[36] and endogenous ADAMTS13 partially protects WT mice in colitis as compared with mice
lacking ADAMTS13 ([Figs. 1] and [2]), we hypothesized that systemic administration of rhADAMTS13 to supplement endogenous
ADAMTS13 in WT mice may further reduce colitis severity.
We intravenously administered rhADAMTS13 or saline vehicle daily, beginning on day
3 of DSS treatment, which is 2 days before the onset of weight loss in the DSS model.
In mice treated with rhADAMTS13, we found less weight loss ([Fig. 6A]), and less severe clinical colitis scores on days 8 to 10 than in vehicle-treated
mice ([Fig. 6B]). While we did observe the expected drop in hemoglobin typical of colitis with bloody
diarrhea in DSS-treated mice, there was not a significant difference in hemoglobin
concentrations ([Fig. 6C]) or onset of bloody diarrhea ([Fig. 6E]) between rhADAMTS13 and vehicle-treated mice, indicating an absence of excessive
rectal bleeding as a result of the rhADAMTS13 treatment. We saw the expected increase
in platelet counts in both rhADAMTS13- and vehicle-treated groups with colitis. There
tended to be less thrombocytosis in the rhADAMTS13-treated group, suggesting a possible
anti-inflammatory effect ([Fig. 6D]). We did not find significant differences between rhADAMTS13- and vehicle-treated
groups with respect to colon length, scores of microscopic inflammation of H&E-stained
colon sections, VWF colon staining, plasma VWF, or spleen weight (data not shown).
This suggests that rhADAMTS13 reduced disease severity, but did not completely reverse
the effect of the chemical colitis produced by DSS treatment.
Fig. 6 Treatment of WT mice with rhADAMTS13 improves outcome. (A) Percent of original body weight in WT mice with DSS treated with vehicle (squares)
or rhADAMTS13 (circles). There was significantly less weight loss in rhADAMTS13-treated
mice on days 7 to 10 when colitis is most severe. (B) Colitis scores are more severe on days 8 and 9 in vehicle-treated compared with
rhADAMTS13-treated mice. (C) Hemoglobin concentration decreases with colitis in both vehicle- and rhADAMTS13-treated
mice. However, there is no significant difference in hemoglobin concentration between
the treatment groups. (D) Platelet count increased in both groups with colitis but tended to be lower in the
rhADAMTS13 treatment group. Five of seven mice in each group developed bloody diarrhea
by day 5 and all mice in both groups by day 6. (E) There was no difference in the onset of bloody diarrhea in rhADAMTS13- or vehicle-treated
mice with DSS colitis. (F) Plasma IL-6, a proinflammatory and prothrombotic cytokine, was lower in rhADAMTS13-treated
compared with vehicle-treated DSS colitic mice. IL-6 also increased with colitis in
vehicle-treated mice but did not differ from baseline in rhADAMTS13-treated mice.
In all graphs, mean and SEM are shown. *p < 0.05, **p < 0.005, *** p < 0.0005.
No differences in IL-10, TAT, or sPsel were detected between rhADAMTS13- and vehicle-treated
mice ([Fig. S1B–D], supplementary figure available in the online version only). However, administration
of rhADAMTS13 resulted in lower IL-6 levels in plasma ([Fig. 6F]). IL-6 is a pathologically important cytokine in human and murine colitis.[37] It is both proinflammatory and prothrombotic, as it enhances platelet activation
and aggregation, increases platelet leukocyte aggregates, and accelerates thrombopoiesis
in DSS colitis.[32]
[37] This finding may in part explain the beneficial effect of rhADAMT13 in the treated
mice ([Fig. 6A, B]).
Discussion
Our findings show an important protective role of ADAMTS13 in colitis and provide
an additional mechanism linking thrombosis and inflammation in IBD. Excessive VWF
secretion from Weibel-Palade bodies has been shown to be highly proinflammatory in
animal models, such as ischemia/reperfusion injury in stroke and myocardial infarction.
It appears that stress signals causing ULVWF release could also precipitate worsening
of colitis by increasing inflammation and microthrombosis. Submucosal thrombi are
reported in colonic biopsies from patients with IBD and not from healthy controls.[38] Additionally, mesenteric venous thrombi are seen on computed tomography scans from
patients with IBD and are frequently associated with areas of inflamed intestine.[39] Both mesenteric vein thrombosis and mesenteric arterial thrombosis inducing ischemia
are known complications of IBD.[40] Our study suggests a mechanism whereby colonic inflammation releases ULVWF, which,
until cleared by ADAMTS13, promotes leukocyte recruitment and entangles platelets.
This results in the formation of VWF-rich thrombi in submucosal vessels. Subsequent
ischemia then causes more Weibel-Palade bodies to release,[41] fueling the vicious circle of inflammation. Administration of pharmacological doses
of ADAMTS13 to WT mice may more effectively interrupt this vicious cycle than the
endogenous enzyme.
We found increased VWF release with colonic inflammation in murine DSS colitis, in
several models of chronic murine colitis, and in inflamed human colon tissue samples.
VWF release was most marked at ulcerated sites. Multiple factors, besides hypoxia,
may contribute to endothelial activation in colitis; circulating platelet leukocyte
aggregates, circulating immune complexes, infection, and cytokines (all of which could
lead to Weibel-Palade body release).
Increased vascular permeability is a feature of colitis[24] and likely results from a multitude of factors, some of which are mentioned earlier.
Thioglycollate peritonitis leads to increased vascular permeability of mesenteric
vessels.[35] In this model, antibody blockade of VWF significantly decreased vascular permeability.[35] Our group has also observed that VWF release under inflammatory conditions increases
the permeability of cerebral vessels.[42] Thus, VWF release likely also affects colonic vessel permeability in colitis.
Excess VWF retained in colonic vessels may contribute to the poorer colitis phenotype
we found in ADAMTS13−/− mice. We also found more VWF staining in inflamed human tissue specimens from patients
with IBD compared with tissue from healthy controls ([Fig. 5]). There are minimal previous studies on VWF staining of human colon tissue, and
they report varied findings. One shows an increased VWF expression in colon tissue
from patients with Crohn's disease,[43] while a second study found no difference in VWF staining.[44] These varied findings may be a result of the heterogeneity of IBD itself, and the
patchy distribution of the disease.
Our study also revealed prominent VWF staining in several chronic murine colitis models
([Fig. 4]). In these models, the severity of the colitis phenotype seem to parallel the amount
of VWF staining present. Similar to the human specimens, more VWF release was seen
in more severely affected sites. These results suggest a generalizability of our findings,
beyond the acute DSS colitis chemical injury model, to diseases of chronic intestinal
inflammation, which involve both the innate and adaptive immune systems, as well as
repeated immune injury, and tissue remodeling.
Our findings of increased plasma VWF in WT mice with colitis coincide with observations
in human subjects, where high circulating VWF levels are found with active colitis.[21] Remarkably, patients with von Willebrand disease or hemophilia have a far lower
risk of developing IBD.[45] This further suggests a deleterious role of VWF in colonic inflammation. VWF represents
an intriguing biomarker, which could be measured in patients with IBD as an indicator
of both disease activity and thrombotic risk. In this study, we have demonstrated
marked VWF release in tissue from patients with active IBD ([Fig. 5]). Interestingly, one clinical study showed lower plasma ADAMTS13 activity associated
with elevated CRP in patients with IBD compared with healthy controls.[11] However, why ADAMTS13 activity may be lower in IBD[11] is not clear. If it is produced by a mutation or polymorphism, it could be a genetic
modifier predisposing to the disease. Low activity could also be the result of the
disease, as the enzyme might be consumed by excessive release of VWF and VWF-mediated
platelet aggregate formation. High circulating VWF and decreased ADAMTS13 activity
could be used as indicators for patients who would benefit from anticoagulation and/or
rhADAMTS13 to decrease their thrombosis risk with an added anti-inflammatory effect.
In our study, treatment of WT mice with rhADAMTS13 decreased colitis severity. Of
note, despite active colonic bleeding in colitis, rhADAMTS13 treatment did not worsen
anemia. This is an important feature if it is to be considered as an adjunct treatment
in colitis.
Thrombocytosis is an inflammatory marker in colitis.[8] The trend toward less thrombocytosis in rhADAMTS13-treated mice suggests an anti-inflammatory
effect. Additionally, rhADAMTS13 treatment resulted in a reduction of plasma IL-6.
IL-6 is a proinflammatory cytokine associated with both murine and human colitis.[46] IL-6 increases platelet production, and these newly formed platelets are more thrombogenic.[10]
[47] Intermittent ischemia and reperfusion occur in the affected areas of the colon.
rhADAMTS13 treatment improves ischemia/reperfusion injury in animal models of stroke[48] and myocardial infarction,[29]
[49]
[50] both of which are VWF mediated. These examples, combined with the observed release
of VWF in our study, support another potential anti-inflammatory mechanism for rhADAMTS13
in colitis along the ADAMTS13-VWF axis.
Our findings suggest that VWF and ADAMTS13 play significant roles in modulating colitis.
Elevated plasma VWF and decreased ADAMTS13 levels or activity are intriguing biomarkers
of inflamed endothelium and thrombotic risk in patients with IBD, which should be
further investigated in human subjects. In patients with low ADAMTS13, rhADAMTS13
could provide additional anti-inflammatory and antithrombotic effects, thus improving
upon existing immunomodulatory therapies used in IBD.
