Development of a Sustainable Procedure for the Recovery of Hydroxytyrosol from Table Olive Processing Wastewater Using Adsorption Resin Technology and Centrifugal Partition Chromatography

Nikos Xynos; Dennis Abatis; Aikaterini Argyropoulou; Panagiotis Polychronopoulos; Nektarios Aligiannis; Alexios-Leandros Skaltsounis

doi:10.1055/s-0035-1558111

Planta Medica, Inhaltsverzeichnis

Planta Med 2015; 81(17): 1621-1627
DOI: 10.1055/s-0035-1558111

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Development of a Sustainable Procedure for the Recovery of Hydroxytyrosol from Table Olive Processing Wastewater Using Adsorption Resin Technology and Centrifugal Partition Chromatography

Nikos Xynos

Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece

,

Dennis Abatis

Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece

,

Aikaterini Argyropoulou

Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece

,

Panagiotis Polychronopoulos

Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece

,

Nektarios Aligiannis

Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece

,

Alexios-Leandros Skaltsounis

Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece

› Institutsangaben

Abstract

The present endeavor aims to establish a novel procedure, applicable to the extraction and isolation of hydroxytyrosol from table olive processing wastewater. A two-step chromatographic separation is presented using non-ionic absorbent resin for the recovery of its phenolic content, followed by purification of hydroxytyrosol with centrifugal partition chromatography. Two table olive processing wastewaters, obtained from Kalamon and Amfissis olive varieties, were used. In the extracts obtained after resin treatment, the hydroxytyrosol content was determined by high-performance liquid chromatography with diode-array detection to be 4.05 % and 10.10 %, respectively. The extract from Amfissis table olive processing wastewater was further processed with preparative centrifugal partition chromatography for the purification of hydroxytyrosol. High-performance liquid chromatography analysis revealed that the isolated compound was > 95 % purity.

Key words

adsorption resin - countercurrent chromatography - centrifugal partition chromatography - hydroxytyrosol - table olive processing wastewater

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