Int J Angiol 2014; 23(03): 193-196
DOI: 10.1055/s-0034-1387825
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Edaravone Injected at the Start of Reperfusion Suppresses Myonephropathic Metabolic Syndrome in Rats

Mitsuhiro Yamamura
1   Department of Cardiovascular Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
,
Yuji Miyamoto
1   Department of Cardiovascular Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
,
Masataka Mitsuno
1   Department of Cardiovascular Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
,
Hiroe Tanaka
1   Department of Cardiovascular Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
,
Masaaki Ryomoto
1   Department of Cardiovascular Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan
› Author Affiliations
Further Information

Publication History

Publication Date:
28 August 2014 (online)

Abstract

The purpose of this study was to evaluate whether edaravone (Radicut®, Mitsubishi Tanabe Pharma Co., Osaka, Japan) injected at the start of reperfusion can suppress myonephropathic-metabolic syndrome (MNMS). MNMS models were made by clamping the bilateral common femoral arteries for 5 hours. At de-clamping (at the start of reperfusion), they were intra-peritoneal injected with 9.0 mg/kg of edaravone (the edaravone group, n = 5) or an equal volume of saline (the control group, n = 5). At five hours after de-clamping, the lower extremity muscles were stained with hematoxylin & eosin (H&E) to count the viable cells, and periodic acid- Schiff (PAS) to assess the glycogen storage. The lungs were also stained with H&E to expresse the alveolar wall thickness, and naphthol AS-D chloroacetate esterase to label infiltrating active neutrophils.

The viable muscle cells in the edaravone group was significantly greater than that of the control group (593 ± 60 vs. 258 ± 31 cells/mm2, p < 0.01). The PAS-positive area in the edaravone group was also significantly higher than that in the control group (30.1 ± 6.9 vs. 7.3 ± 2.1%, p < 0.001). The alveolar wall thickness in the edaravone group was significantly lower than that in the control group (63.6 ± 5.6 vs. 17.2 ± 5.2%, p < 0.001). The active neutrophil infiltration in the edaravone group was also significantly lower than that in the control group (249 ± 59 vs. 68 ± 8 cells/mm2, p < 0.001).

We conclude that edaravone injected at the start of reperfusion can suppress not only muscle reperfusion injury but also lung damage.

Note

This study was presented at the Arteriosclerosis Thrombosis and Vascular Biology Scientific Sessions, Chicago on April 2012 and Florida on April 2013, and the 55th Annual World Congress of International College of Angiology, New Haven on November 2013.


 
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