Drug Res (Stuttg) 2015; 65(04): 219-222
DOI: 10.1055/s-0034-1382076
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Genotoxic Effects of some Antituberculosis Drugs and Mixtures in Rats

K. Arslan
1   Erciyes University, Veterinary Faculty, Department of Genetic, Kayseri, Turkey
,
M. Kanbur
2   Erciyes University, Veterinary Faculty, Department of Pharmacology and Toxicology, Kayseri, Turkey
,
M. Karabacak
3   Erciyes University, Safiye Çıkrıkçıoğlu Vocational College, Kayseri, Turkey
,
Z. S. Sarıca
4   Erciyes University, Experimental and Clinical Research Center, Kayseri, Turkey
,
N. Taşçıoğlu
5   Erciyes University, Faculty of Medicine, Department of Genetic, Kayseri, Turkey
,
K. M. İşcan
6   Erciyes University, Veterinary Faculty, Department of Zootecnics, Kayseri, Turkey
,
M. Dündar
5   Erciyes University, Faculty of Medicine, Department of Genetic, Kayseri, Turkey
,
A. Akçay
7   Erciyes University, Veterinary Faculty, Department of Biometrics, Kayseri, Turkey
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 22. April 2014

accepted 04. Juni 2014

Publikationsdatum:
03. Juli 2014 (online)

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Abstract

In this study[*], it was aimed to observe, genotoxic effects of antituberculosis drugs and combinations on rats. Animals were treated with 31.5 mg/kg isoniazid (INH), 54 mg/kg rifampicin (RIF), 189 mg/kg pyrazinamide (PYR), 100 mg/kg etham­butol(ETA), INH+RIF+PYR (MIX1) and INH+RIF+PYR+ETA (MIX2) mixtures applied via gavage for 90 days. At the end of the study, blood, liver and kidney samples were taken and evaluated by Comet and Micronucleus techniques. Compared to control group, head intensity decreased, tail intensity and tail migration increased on experiment groups in blood samples. Head intensity of PYR and mixture groups decreased, tail intensity of PYR and mixture groups increased and tail migration of PYR, ETA and mixture groups increased in liver samples. Head intensity decreased and tail intensity increased of INH, RIF, ETA and MIX1 group; tail migration increased of MIX1 group in kidney samples. Compared to control group, micronucleus rate of ETA, RIF and MIX 2 groups increased in experiment groups. In conclusion antituberculosis drugs and their mixtures applied for 90 days causes to double strand break of DNA damage at different degrees in blood, kidney and liver cells in rats.

* This study was supported by the Erciyes University Scientific Research Projects Unit, within the scope of the project with the number of “TSA-10–3262 and presented to 8th Society of Turkish Toxicology Congress as poster presentation.