Planta Med 2014; 80(05): 393-398
DOI: 10.1055/s-0033-1360383
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Protective Effects of Crocin on Hemodynamic Parameters and Infarct Size in Comparison with Vitamin E after Ischemia Reperfusion in Isolated Rat Hearts

Mahin Dianat
1   Department of Physiology and Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
,
Mahdi Esmaeilizadeh
2   Ph.D. Student, Department of Physiology and Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
,
Mohammad Badavi
1   Department of Physiology and Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
,
Alireza Samarbafzadeh
3   Department of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
,
Bahareh Naghizadeh
4   Department of Pharmacology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
› Author Affiliations
Further Information

Publication History

received 29 July 2013
revised 23 January 2014

accepted 27 January 2014

Publication Date:
28 February 2014 (online)

Abstract

Although reperfusion is a useful method for the survival of ischemic heart, harmful effects have been observed. This study was carried out to investigate the preconditioning and cardioprotective potential effects of crocin and vitamin E on the hemodynamic and infarct size in the ischemia-reperfusion model of isolated rat hearts. Animals were divided into a control group, an ischemia-reperfusion control group and three treatment groups: crocin (10, 20, and 40 mg/kg), vitamin E (100 mg/kg), and combination (crocin 40 mg/kg with vitamin E 100 mg/kg). The hearts were excised, quickly transferred to a Langendorff apparatus, and subjected to 30 min of global ischemia followed by 60 min of reperfusion. Left ventricular developed pressure, coronary perfusion pressure, left ventricular systolic pressure, myocardial contractility, rate pressure product, coronary flow, and infarct size were assessed. The successful induction of ischemia was determined by ST elevation on the electrocardiogram.

The results showed that crocin significantly improved cardiac dysfunction and also reduced infarct size in the rat hearts. However, the combination of crocin 40 mg/kg and vitamin E 100 mg/kg had an even more significantly improved effect on the hemodynamic parameters and infarct size.

Therefore, it can be suggested that the protective role of crocin may be due to the stability or reinforcement of antioxidant systems, and crocin could be useful for the treatment or prevention of cardiac dysfunction.

 
  • References

  • 1 Canbaz S, Duran E, Ege T, Sunar H, Cikirikcioglu M, Acipayam M. The effects of intracoronary administration of vitamin E on myocardial ischemia-reperfusion injury during coronary artery surgery. Thorac Cardiovasc Surg 2003; 51: 57-61
  • 2 Kinugasa Y, Ogino K, Furuse Y, Shiomi T, Tsutsui H, Yamamoto T, Igawa O, Hisatome I, Shigemasa C. Allopurinol improves cardiac dysfunction after ischemia-reperfusion via reduction of oxidative stress in isolated perfused rat hearts. Circ J 2003; 67: 781-782
  • 3 Dhalla NS, Elmoselhi AB, Hata T, Makino N. Status of myocardial antioxidants in ischemia-reperfusion injury. Cardiovasc Res 2000; 47: 446-456
  • 4 Jahanbakhsh Z, Rasoulian B, Jafari M, Shekarforoush SH, Esmailidehaj M. Protective effect of crocin against reperfusion-induced cardiac arrhythmias in anaesthetized rats. EXCLI J 2012; 11: 20-29
  • 5 Montagna G, Hofer CG, Torres AM. Impairment of cellular redox status and membrane protein activities in kidneys from rats with ischemic acute renal failure. Biochem Biophys Acta 1998; 14: 99-108
  • 6 Hosseinzadeh H, Sadeghnia HR, Ziaee T, Danaee A. Protective effect of aqueous saffron extract (Crocus sativus L.) and crocin, its active constituent, on renal ischemia-reperfusion-induced oxidative damage in rats. J Pharm Pharm Sci 2005; 8: 387-393
  • 7 Upaganlawar A, Balaraman R. Effect of vitamin E and green tea on hemodynamic, electro cardiographic and some biochemical alterations in experimentally induced myocardial infarction in rats. Eur J Integr Med 2010; 2: 135-141
  • 8 Ochiai T, Shimeno H, Mishima K. Protective effects of carotenoids from saffron on neuronal injury in vitro and in vivo . Biochim Biophys Acta 2007; 170: 578-584
  • 9 Shen XC, Qian ZY. Effects of crocetin on antioxidant enzymatic activities in cardiac hypertrophy induced by norepinephrine in rats. Pharmazie 2006; 61: 348-352
  • 10 Fernández J. Anticancer properties of saffron, Crocus sativus Linn. Adv Phytomed 2006; 2: 313-330
  • 11 Assimopoulou AN, Sinakos Z, Papageorgiou V. Radical scavenging activity of Crocus sativus L. extract and its bioactive constituents. Phytother Res 2005; 19: 997-1000
  • 12 Hosseinzadeh H, Shamsaie F, Mehri S. Antioxidant activity of aqueous and ethanolic extracts of Crocus sativus L. stigma and its bioactive constituent, crocin and safranal. Pharmacogn Mag 2009; 5: 419-424
  • 13 Pour ZM, Zal F, Monabati A, Vessal MO. Protective effects of a combination of quercetin and vitamin E against cyclosporine A-induced oxidative stress and hepatotoxicity in rats. Hepatol Res 2008; 38: 385-392
  • 14 Zhou BO, Wu LM, Yang LI, Liu ZL. Evidence for alpha-tocopherol regeneration reaction of green tea polyphenols in SDS micelles. Free Radic Biol Med 2005; 38: 78-84
  • 15 Midorikawa J, Maehara K, Yaoita H, Watanabe T, Ohtani H, Ushiroda S. Continuous observation of superoxide generation in an in-situ ischemia–reperfusion rat lung model. Jpn Circ J 2001; 65: 207-212
  • 16 Osada M, Netticadan T, Tamura K, Dhalla NS. Modification of ischemia–reperfusion induced changes in cardiac sarcoplasmic reticulum by preconditioning. Am J Physiol 1998; 274: 2025-2034
  • 17 Goldspink DF, Burniston JG, Ellison GM, Clark WA, Tan LB. Catecholamine-induced apoptosis and necrosis in cardiac and skeletal myocytes of the rat in vivo: the same or separate death pathways. Exp Physiol 2004; 89: 407-416
  • 18 Abdullaev FI, Espinosa-Aguirre JJ. Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detect Prev 2004; 28: 426-432
  • 19 Srivastava S, Chandrasekar B, Gu Y, Luo J, Hamid T, Hill BG. Downregulation of CuZn-superoxide dismutase contributes to beta-adrenergic receptor-mediated oxidative stress in the heart. Cardiovasc Res 2007; 74: 445-455
  • 20 Zhang R, Qian ZY, Han XY, Chen Z, Yan JL, Hamid A. Comparison of the effects of crocetin and crocin on myocardial injury in rats. Chin J Nat Med 2009; 7: 223-227
  • 21 Lee JM, Cook NR, Gaziano JM, Gordon D, Ridker PM, Manson JE, Hennekens CH, Buring JE. Vitamin E in the primary prevention of cardiovascular disease and cancer. JAMA 2005; 294: 56-65
  • 22 Behl C, Moosmann B. Antioxidant neuroprotection in Alzheimerʼs disease as preventive and therapeutic approach. Free Radic Biol Med 2002; 33: 182-191
  • 23 Goyal NS, Arora S, Sharma AK, Joshi S, Ray R. Preventive effect of crocin of Crocus sativus on hemodynamic, biochemical, histopathological and ultrastructural alterations in isoproterenol-induced cardiotoxicity in rats. Phytomedicine 2010; 17: 227-232
  • 24 Garcıa-Villalon AL, Monge L, Fernandez N, Salcedo A. Coronary response to diadenosine pentaphosphate after ischaemia–reperfusion in the isolated rat heart. CardiovascRes 2009; 81: 336-343
  • 25 Pourkhalili K. Evaluation of the effects of normobaric hyperoxia pretreatment on ischemia-reperfusion injury in a regional ischemia model using isolated rat heart. J Physiol Pharmacol 2008; 12: 91-100