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Planta Med 2014; 80(02/03): 187-192
DOI: 10.1055/s-0033-1360261
Pharmacokinetic Investigations
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Pharmacokinetic Interaction of Astragaloside IV with Atractylenolide I and Prim-O-Glucosylcimifugin in Male Sprague Dawley Rats

Jue Song
School of Pharmacy, Anhui Medical University, Hefei, Anhui, Peopleʼs Republic of China
,
Shi-rui Zheng
School of Pharmacy, Anhui Medical University, Hefei, Anhui, Peopleʼs Republic of China
,
Yong Jin
School of Pharmacy, Anhui Medical University, Hefei, Anhui, Peopleʼs Republic of China
,
Jun Li
School of Pharmacy, Anhui Medical University, Hefei, Anhui, Peopleʼs Republic of China
› Author Affiliations
Further Information

Publication History

received 02 November 2012
revised 04 December 2013

accepted 06 December 2013

Publication Date:
22 January 2014 (online)

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Abstract

Astragaloside IV, atractylenolide I, and prim-O-glucosylcimifugin are main medicinal components of the traditional Chinese medicine prescription Yu-ping-feng which is composed of three herbs: Astragalus membranaceus, Atractylodes macrocephala, and Saposhnikovia divaricata. This study is aimed to assess the influence of atractylenolide I and prim-O-glucosylcimifugin on the pharmacokinetic profile of astragaloside IV so as to investigate the pharmacokinetic mechanisms of the Yu-ping-feng prescription. Fifteen Sprague Dawley rats were randomized to three groups; astragaloside IV, astragaloside IV plus atractylenolide I, and a combination of astragaloside IV, atractylenolide I, and prim-O-glucosylcimifugin were respectively administered to rats of these three groups via intragastric gavage. Serum samples were collected at different times after drug administration, and serum concentrations of astragaloside IV and atractylenolide I were simultaneously detected using HPLC-electrospray ionization-MS. Compared with administration of astragaloside IV alone, concentrations of astragaloside IV in the serum were significantly increased when it was given in combination with atractylenolide I or atractylenolide I+prim-O-glucosylcimifugin, with higher values for Cmax (p = 0.019 and p = 0.033 compared with astragaloside IV + atractylenolide I and astragaloside IV + atractylenolide I + prim-O-glucosylcimifugin groups, respectively) and AUC (p = 0.0052 and p = 0.0047 compared with astragaloside IV + atractylenolide I and astragaloside IV + atractylenolide I + prim-O-glucosylcimifugin groups, respectively). Improvement in mean oral Cmax and mean systemic serum exposure because of the pharmacokinetic interaction between astragaloside IV and atractylenolide I might explain the rationale for the use of multiple herbs in Yu-ping-feng and of combinations of A.membranaceus and A. macrocephala.

Key words

pharmacokinetic interaction - astragaloside IV - atractylenolide I - Astragalus membranaceus - Atractylodes macrocephala - Yu-ping-feng - Leguminosae - Compositae - Umbelliferae

Supporting Information

 

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