Exp Clin Endocrinol Diabetes 2013; 121(10): 607-613
DOI: 10.1055/s-0033-1354380
Article
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

p21 is Associated with the Proliferation and Apoptosis of Bone Marrow-derived Mesenchymal Stem Cells from Non-obese Diabetic Mice

Z. Gu
1   Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, P. R. China
,
J. Jiang
1   Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, P. R. China
,
Y. Xia
1   Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, P. R. China
,
X. Yue
2   State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, P. R. China
,
M. Yan
1   Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, P. R. China
,
T. Tao
3   Department of Immunology, Medical College, Nantong University, ­Nantong, P. R. China
,
X. Cao
4   Department of Pathology, Nantong University, Nantong, P. R. China
,
Z. Da
1   Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, P. R. China
,
H. Liu
5   Department of Hematology, Affiliated Hospital of Nantong University, Nantong, P. R. China
,
H. Liu
1   Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, P. R. China
,
Y. Miao
6   Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P. R. China
,
L. Li
7   Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, P. R. China
,
Z. Wang
8   Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, P. R. China
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Publikationsverlauf

received 10. Mai 2013
first decision 08. August 2013

accepted 14. August 2013

Publikationsdatum:
25. November 2013 (online)

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Abstract

Recent studies have shown that autologous and allogeneic transplantation of the BM-MSCs had therapeutic effects on T1DM, whereas the BM-MSCs from the NOD mice itself did not have this therapeutic effect. We previously demonstrated that Bone Marrow (BM) -MSCs from the non-obese diabetic (NOD) mice had the abnormal migration and adhesion. So we hypothesized that the proliferation and apoptosis of the BM-MSCs from the NOD mice were dysregulated. Our team compared the proliferation and apoptosis between NOD mice and imprinting control region (ICR) mice. Then we assessed whether the NF-κB-p53/p21 pathway was involved in the process. The cell proliferation ability of the BM-MSCs from the NOD mice were significantly decreased, while the percent of apoptotic cells was increased compared to those from the ICR mice. The p21 expression was significantly increased in the NOD-MSCs. The p65 level was enhanced in the BM-MSCs from the NOD mice when compared to the ICR mice, coincided with the expression of p21. Expressions of p65 and p21 were significantly decreased in the ­BM-MSCs treated with p65 inhibitor. The knockdown p21 expression reversed the abnormal proliferation, colony formation and apoptosis of the BM-MSCs from the NOD mice. These data provide important preclinical references supporting the basis for further development of autologous MSC-based therapies for type1 diabetes mellitus (T1DM).