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Planta Med 2013; 79(17): 1653-1655
DOI: 10.1055/s-0033-1351022
Biological and Pharmacological Activity
Letters
Georg Thieme Verlag KG Stuttgart · New York

In Vitro Antiparasitic Activity and Chemical Composition of the Essential Oil Obtained from the Fruits of Piper cubeba

Viviane Rodrigues Esperandim
1   University of Franca, Franca, São Paulo, Brazil
,
Daniele da Silva Ferreira
1   University of Franca, Franca, São Paulo, Brazil
,
Karen Cristina Sousa Rezende
1   University of Franca, Franca, São Paulo, Brazil
,
Lizandra Guidi Magalhães
1   University of Franca, Franca, São Paulo, Brazil
,
Julia Medeiros Souza
1   University of Franca, Franca, São Paulo, Brazil
,
Patrícia Mendonça Pauletti
1   University of Franca, Franca, São Paulo, Brazil
,
Ana Helena Januário
1   University of Franca, Franca, São Paulo, Brazil
,
Rosangela da Silva de Laurentz
2   College of Engineer of Ilha Solteira (UNESP), Ilha Solteira, São Paulo, Brazil
,
Jairo Kenupp Bastos
3   School of Pharmaceutical Sciences of Ribeirão Preto (USP), Ribeirão Preto, São Paulo, Brazil
,
Guilherme Venâncio Símaro
1   University of Franca, Franca, São Paulo, Brazil
,
Wilson Roberto Cunha
1   University of Franca, Franca, São Paulo, Brazil
,
Márcio Luis Andrade e Silva
1   University of Franca, Franca, São Paulo, Brazil
› Author Affiliations
Further Information

Publication History

received 27 June 2013
revised 02 October 2013

accepted 04 October 2013

Publication Date:
28 November 2013 (online)

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Abstract

Protozoans of the trypanosomatid family cause the neglected tropical diseases leishmaniasis and trypanosomiasis, for which few drugs are available. In this context our group has recently reported that the essential oil obtained by steam distillation of the fruits of Piper cubeba is active against Schistosoma mansoni. Therefore, we have investigated the in vitro effects of the essential oil against the trypomastigote and amastigote forms of Trypanosoma cruzi isolated from an LLCMK2 cell line culture and the promastigote forms of Leishmania amazonensis. The in vitro activity of the essential oil against trypomastigotes of T. cruzi increased upon rising concentrations, giving IC50 values of 45.5 and 87.9 µg · mL−1 against trypomastigotes and amastigotes, respectively. The essential oil was not active against L. amazonensis, since it displayed lyses of only 24 % at 400 µg · mL−1, and an IC50 of 326.5 µg · mL−1. Therefore, the essential oil should be further investigated to determine the compounds responsible for the observed activities, as well as its mechanism of action.

Key words

Piper cubeba - Piperaceae - essential oil - trypanocidal activity - leishmanicidal activity

Supporting Information

 

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