Drug Res (Stuttg) 2013; 63(09): 473-476
DOI: 10.1055/s-0033-1345183
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

A Reliable Method to Quantify Sildenafil and its Metabolite N-Demethylsildenafil by HPLC in Plasma of Children

Authors

  • B. Ramírez-Mendiola

    1   Laboratorio de Farmacología, Instituto Nacional de Pediatría, Mexico City, Mexico
  • C. Flores-Pérez

    1   Laboratorio de Farmacología, Instituto Nacional de Pediatría, Mexico City, Mexico
  • R. García-Álvarez

    1   Laboratorio de Farmacología, Instituto Nacional de Pediatría, Mexico City, Mexico
  • H. Juárez-Olguín

    1   Laboratorio de Farmacología, Instituto Nacional de Pediatría, Mexico City, Mexico
    2   Departamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico
  • J. Flores-Pérez

    1   Laboratorio de Farmacología, Instituto Nacional de Pediatría, Mexico City, Mexico
    2   Departamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico
  • J. L. Chávez Pacheco

    1   Laboratorio de Farmacología, Instituto Nacional de Pediatría, Mexico City, Mexico
Further Information

Publication History

received 29 January 2013

accepted 25 April 2013

Publication Date:
04 June 2013 (online)

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Abstract

The aim of this study was to develop a simple method for quantifying plasma levels of sildenafil and its metabolite by liquid chromatography with a C18 reverse-phase column and UV detection. For both compounds, linearity was assessed in the range from 10 and 1 000 ng · ml−1 and had correlation coefficients of r=0.995 and r=0.997 for sildenafil and its metabolite, respectively. The inter- and intra-day coefficients of variation was<5.3%. The limits of detection and quantification were 1 and 10 ng · ml−1. Drug levels were determined satisfactorily in two patients. A simple and reliable method was developed for use in children with Pulmonary Arterial Hypertension under treatment with sildenafil.