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Exp Clin Endocrinol Diabetes 2013; 121(07): 436-439
DOI: 10.1055/s-0033-1345124
Article
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

C332C Genotype of Glyoxalase 1 and its Association with Late Diabetic Complications

Glo1 Genotype and Diabetic Neuropathy
J. B. Groener
1   Department of Medicine, Division of Endocrinology and Clinical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany
,
P. Reismann
1   Department of Medicine, Division of Endocrinology and Clinical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany
,
T. Fleming
1   Department of Medicine, Division of Endocrinology and Clinical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany
,
H. Kalscheuer
1   Department of Medicine, Division of Endocrinology and Clinical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany
,
D. Lehnhoff
1   Department of Medicine, Division of Endocrinology and Clinical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany
,
A. Hamann
1   Department of Medicine, Division of Endocrinology and Clinical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany
,
P. Roser
1   Department of Medicine, Division of Endocrinology and Clinical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany
,
A. Bierhaus
†   Deceased
,
P. P. Nawroth
1   Department of Medicine, Division of Endocrinology and Clinical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany
,
G. Rudofsky
1   Department of Medicine, Division of Endocrinology and Clinical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany
› Author Affiliations
Further Information

Publication History

received 08 January 2013
first decision 08 March 2013

accepted 15 April 2013

Publication Date:
17 June 2013 (online)

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Abstract

Aims/Introduction:

Glyoxalase 1 catalyses the detoxification of methylglyoxal, a major precursor of advanced glycation end products associated with aging, neurodegenerative diseases, and microvascular complications of diabetes. Here, we examine a possible association of a single nucleotide polymorphism of glyoxalase 1 gene (Glo1 A332C, rs4746 or rs2736654) with the prevalence of microvascular diabetic complications in patients with type 1 and type 2 diabetes.

Materials and Methods:

Genotyping was performed in 209 patients with type 1 and 524 patients with type 2 diabetes using polymerase chain reaction and subsequent cleavage by restriction endonuclease Bsa I.

Results:

Frequencies of the glyoxalase 1 genotypes were different with respect to diabetes type with a significantly higher prevalence of A332A-genotype in type 1 diabetes (35.9% vs. 27.3%; p=0.03). In type 1 diabetes, there was no correlation of any genotype with diabetic retinopathy, nephropathy or neuropathy. In contrast, type 2 diabetic patients homozygous for the C332C allele showed a significantly increased prevalence of diabetic neuropathy (p=0.03; OR=1.49 [95%-CI: 1.04; 2.11]), while no association with diabetic nephropathy or retinopathy was found. However, the significance of this association was lost after correction for multiple testing.

Conclusions:

Our data suggest a possible association of C332C-genotype of the glyoxalase 1 gene with diabetic neuropathy in type 2 diabetes, supporting the hypothesis that methylglyoxal might be an important mediator of diabetic neuropathy in type 2 diabetes.

Key words

diabetic neuropathy - detoxification - methylglyoxal - diabetes - Glo1 A332C - rs4746
 

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