Ex-vivo-Expansion von Erythrozyten – aktueller Stand

Zusammenfassung

Die Ex-vivo-Expansion von Erythrozyten steht an der Schwelle zum klinischen Einsatz. Damit steht ein Traum der Transfusionsmedizin, Erythrozyten gezielt produzieren zu können, kurz vor der Verwirklichung. Diese Übersicht fasst die aktuellen Entwicklungen im Bereich der Physiologie der Erythropoese im Knochenmark, der Zellkulturprotokolle im Übergang vom Labormaßstab in den Bereich der Guten Herstellungspraxis (GMP), der verschiedenen Quellen für ex vivo expandierte Erythrozyten, die zurzeit verfügbaren klinischen Daten und die zukünftigen Möglichkeiten der Anwendung zusammen.

Abstract

Ex vivo expansion of erythrocyte precursors to erythrocyte concentrates is at the cusp to clinical practice. An old dream of transfusionists, the in vitro manufacturing of erythrocytes seems to come true. This review summarises the current status in the physiology of bone-marrow derived erythropoiesis, the recent protocols in erythrocyte cell culture in the transit from laboratory scale to good manufacturing practice, the different sources for the ex vivo expansion of cells for this purpose, the most recent clinical data and the prospective scope in therapeutical administration.

Schlüsselwörter

Erythropoese - Erythrozytenkonzentrate - Stammzellen - Ex-vivo-Expansion

Key words

erythropoiesis - red blood cell concentrate - stem cells - ex vivo expansion

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