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Planta Med 2012; 78(7): 711-719
DOI: 10.1055/s-0031-1298380
Natural Product Chemistry
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Neolignans and Sesquiterpenes from Cell Cultures of Stellera chamaejasme

Li-Rui Qiao1 , Lin Yang1 , Jian-Hua Zou1 , Li Li1 , Hua Sun1 , Yi-Kang Si1 , Dan Zhang1 , Xiaoguang Chen1 , Jungui Dai1
  • 1State Key Laboratory of Bioactive Substances and Function of Natural Medicines and Key Laboratory of Biosynthesis of Natural Products, Ministry of Health, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China
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Publikationsverlauf

received Dec. 5, 2011 revised February 5, 2012

accepted February 22, 2012

Publikationsdatum:
22. März 2012 (online)

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Abstract

Two new neolignans (1, 2) and six sesquiterpenes (3–8) were isolated from the cell cultures of Stellera chamaejasme. Their structures and absolute configurations were elucidated by extensive spectroscopic and computational methods. Compound 4 exhibited significant protective effects against CCl4-induced hepatotoxicity in HepG2 cells, reducing aspartate aminotransferase release by 29.49 % at 10 µM. These compounds have not been isolated from plant material, which implies that in vitro plant cell cultures may offer alternative and effective sources of bioactive natural compounds.

Key words

Stellera chamaejasme L. - Thymelaeaceae - callus cultures - sesquiterpenes - density functional theory - CCl4-induced hepatotoxicity

Supporting Information

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Prof. Jungui Dai, Ph.D.

Institute of Materia Medica
Chinese Academy of Medical Sciences & Peking Union Medical College
Biosynthesis of Natural Products

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