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Arzneimittelforschung 2008; 58(9): 441-444
DOI: 10.1055/s-0031-1296536
Antiemetics · Gastrointestinal Drugs · Urologic Drugs
Editio Cantor Verlag Aulendorf (Germany)

A Rapid High-performance Liquid Chromatographic Method for the Determination of Pantoprazole in Plasma Using UV Detection

Application in pharmacokinetic studies
Afshin Zarghi
1   Department of Pharmaceutical Chemistry, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran
,
Alireza Shafaati
1   Department of Pharmaceutical Chemistry, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran
,
Seyed Mohsen Foroutan
2   Department of Pharmaceutics, School of Pharmacy, Shaheed Beheshti 3University of Medical Sciences, Tehran, Iran
,
Hooman Movahed
3   cNoor Research and Educational Institute, Tehran, Iran
,
Arash Khoddam
3   cNoor Research and Educational Institute, Tehran, Iran
› Institutsangaben
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Publikationsverlauf

Publikationsdatum:
19. Dezember 2011 (online)

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Abstract

A rapid, sensitive and reproducible HPLC method was developed and validated for the analysis of pantoprazole (CAS 102625-70-7) in human plasma. The separation was achieved on a monolithic silica column using acetonitrile-potassium dihydrogen phosphate buffer (25:75,v/v), pH 3.0, as the mobile phase at a flow rate of 1.5 ml min−1. The wavelength was set at 290 nm. The assay enables the measurement of pantoprazole for therapeutic drug monitoring with a minimum quantification limit of 20 ng ml−1. The method involves a simple protein precipitation procedure. Analytical recovery was complete. The calibration curve was linear over the concentration range 20–3500 ng ml−1. The coefficients of variation for the inter-day and intra-day assay were found to be less than 7%.

Key words

CAS 102625-70-7 - Pantoprazole, determination in plasma, HPLC-UV, pharmacokinetic studies - Proton pump inhibitors
 

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