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Arzneimittelforschung 2008; 58(9): 429-434
DOI: 10.1055/s-0031-1296534
Cardiac Drugs · Cardiac Stimulants · Coronary Drugs
Editio Cantor Verlag Aulendorf (Germany)

Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Trimetazidine in Human Plasma

Jianjun Zou
1   Department of Clinical Pharmacology, anjing First Hospital Affiliated to Nanjing Medical University, Nanjing, The People’s Republic of China
,
Li Dai
2   Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, The People’s Republic of China
,
Guang Ji Wang
3   Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, The People’s Republic of China
,
Yubing Zhu
1   Department of Clinical Pharmacology, anjing First Hospital Affiliated to Nanjing Medical University, Nanjing, The People’s Republic of China
,
Hongwei Fan
1   Department of Clinical Pharmacology, anjing First Hospital Affiliated to Nanjing Medical University, Nanjing, The People’s Republic of China
,
Da Wei Xiao
1   Department of Clinical Pharmacology, anjing First Hospital Affiliated to Nanjing Medical University, Nanjing, The People’s Republic of China
› Author Affiliations
Further Information

Publication History

Publication Date:
19 December 2011 (online)

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Abstract

A sensitive liquid chromatography-tandem mass spectrometry (LC/MS/MS) method was developed and validated for the determination of trimetazidine (CAS 13171-25-0) in human plasma, using pseudoephedrine as internal standard (IS). Plasma samples were simply pretreated with methanol for deproteinization. The chromatographic separation was performed on a C18 column with a mobile phase of 3 mmol/L ammonium acetate solution-methanol (15:85, v/v) at a flow rate of 0.3 mL/min. The chromatographic separation was achieved in less than 3.2 min. The linearity was established over the concentration range of 1–100 ng/mL. Both intra- and inter-batch standard deviations were less than 9.5%. The method was successfully applied to study the relative bioavailability of trimetazidine hydrochloride tablets in healthy Chinese volunteers and the pharmacokinetic parameters of the reference and test tablets were compared.

Key words

Antianginal drugs - CAS 13171-25-0 - Trimetazidine, bioavailability, LC/MS/MS, pharmacokinetics
 

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