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Arzneimittelforschung 2010; 60(11): 660-666
DOI: 10.1055/s-0031-1296344
Cardiac Drugs · Cardiac Stimulants · Coronary Drugs
Editio Cantor Verlag Aulendorf (Germany)

Study of sodium tanshinone II A sulfonate tissue distribution in rat by liquid chromatography/tandem mass spectrometry

Tingting He
1   Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, P. R., China
1   Center for Instrumental Analysis, China Pharmaceutical University, Nanjing, P. R., China
,
Qiaogen Zou
1   Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, P. R., China
1   Center for Instrumental Analysis, China Pharmaceutical University, Nanjing, P. R., China
1   College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, P. R., China
,
Zhenbin Feng
1   Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, P. R., China
1   Center for Instrumental Analysis, China Pharmaceutical University, Nanjing, P. R., China
,
Zunjian Zhang
1   Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, P. R., China
1   Center for Instrumental Analysis, China Pharmaceutical University, Nanjing, P. R., China
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Publication History

Publication Date:
30 December 2011 (online)

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Abstract

A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC-MS/MS) has been developed and fully validated for the quantitative determination of sodium tanshinone II A sulfonate (STS, sodium (1,6,6-trimethyl-10,11 -dioxo-7,8,9-trihydrophe-nanthro[1,2-b]furan)-yl-2-sulfonate) in rat biosamples including plasma and different tissues using sodium tanshinone I sulfonate (sodium (l,6-dimethyl-10,ll-dioxo-phenanthro[l,2-b]furan)-yl-2-sul-fonate) as internal standard. Simple protein precipitation by acetonitrile was utilized for extracting STS from the rat biosamples. Chromatographic separation of the sample matrix from the analyte and the internal standard was performed using a commercially available analytical column with a mobile phase consisting of methanol-5 mmoL/L ammonia acetate (70:30, v/v) at a flow rate of 0.2 mL/min.

Detection was performed on a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization source and operated in the negative-ion mode. The intra- and inter-day precisions (RSD%) and deviations of the assay accuracies were within 10.0% for STS. The extraction recovery of STS was more than 86.5%. The limit of detection (LOD) of STS was 1.0 ng/mL. The method was successfully applied to the tissue distribution study of STS intravenously administered to healthy Sprague-Dawley rats. The tissue distribution results showed that liver, kidney, lung, small intestine and duodenum were the major distribution tissues of STS in rats, and that STS had difficulty in crossing the blood-brain barrier. After 24 h, STS could be detected only in the kidney, stomach and small intestine, indicating that there was no long-term accumulation of STS in rat.

Key words

Cardiovascular drug - LC-MS/MS - Sodium tanshinone I sulfonate - Sodium tanshinone II A sulfonate, rats, tissue distribution
 

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